Synthesis and characterization of transition metal 2,6-pyridinedicarboxylic acid derivatives, interactions of Cu(II) and Ni(II) complexes with DNA in vitro

Khan, Shabnam; Nami, Shahab A.A.; Siddiqi, K. S.; Husain, Eram; Naseem, Imrana

doi:10.1016/j.saa.2008.10.001

Cited by 60 publications

(21 citation statements)

References 40 publications

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“…This phenomenon indicated that EB was partially replaced by emodin in the EB-DNA system, and EB was released from a hydrophobic environment into the water solution. The result suggested that emodin binds to DNA in the intercalating mode [21,29].…”

Section: Fluorescence Spectra Studymentioning

confidence: 97%

“…Varying concentrations of emodin (0, 50, 100, and 200 lg/ml) were then added to the EB-DNA solution, and the fluorescence spectra were measured for each test solution after 30 min of incubation at 37°C. The solutions were excited at 535 nm, and spectra were recorded from 550 to 720 nm [20,21]. Tests were performed in a 1-cm-path-length quartz cell.…”

Section: Fluorescence Measurementmentioning

confidence: 99%

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Antibacterial properties of anthraquinones extracted from rhubarb against Aeromonas hydrophila

Wang

et al. 2011

Fish Sci

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Antibacterial properties of crude extract from rhubarb and its major bioactive compounds against Aeromonas hydrophila were assayed. Major bioactive compounds (anthraquinone derivatives) in rhubarb collected from different cultivation areas were determined by ultraperformance liquid chromatography (UPLC); the antibacterial activity [minimum inhibitory concentration (MIC)] of rhubarb was positively related to the anthraquinone content (r = 0.9306, P \ 0.01). The MIC values of five anthraquinones against A. hydrophila were found to be in the range 50-200 lg/ml. Action-mode studies showed that anthraquinones (emodin) inhibits cellular functions by binding to cell DNA after penetrating the cell membrane, resulting in cell death. The present study suggests that anthraquinones extracted from rhubarb have potential use as antimicrobials for control of A. hydrophila.

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Section: Fluorescence Spectra Studymentioning

confidence: 97%

Section: Fluorescence Measurementmentioning

confidence: 99%

Antibacterial properties of anthraquinones extracted from rhubarb against Aeromonas hydrophila

Wang

et al. 2011

Fish Sci

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“…Th is is due to the fact that PDC is a versatile chelating agent that can act as a bidentate, tridentate, merid ian or bridge bonding with different metallic ions [44][45][46][47]. PDC also presents other important characteristics, such as low toxicity and its diversified bio logical activ ity being present in many natural products such as oxidative degradation product of vitamins, coenzymes and alkaloids [50,51]. The use of organic iron complexes in catalysis has been pointed to as advantageous by some authors, considering the stabilization of the iron in a wider pH range in relation to that in the absence of complexes.…”

Section: Resultsmentioning

confidence: 99%

Computational Insights into the Role of the Frontiers Orbital in the Chemistry of Tridentate Ligands

Porta¹,

Giacoppo²,

Ramos³

et al. 2012

CHEMISTRY

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The FERM O concept emerges as a powerful and innovative implement to investigate the role of molecular orbitals applied in the description of breakage and formation of chemical bonds. In this work, Hartree-Fock (HF) and Density Functional Theory (DFT) calculations were carried out for a series of four tridentate ligands and their behavior was analyzed using molecular orb ital (M O) energies. It was observed that HOMO energies are inadequate to describe the Pearson´s Hard and Solf acid-base (HSA B) p ricinple behavior of these compounds. By using the frontier effective-for-reaction molecular orbital (FERM O) concept, the reactions that are driven by HOM O, and those that are not, can be better exp lained, independent of the calculation method used, since both HF and Kohn-Sham methodologies lead to the same FERM O.

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“…In fact results of spectroscopic studies can give the information concerning the mode and intensity binding of small molecules to DNA. [26][27][28] EG is a cationic organic dye from triphenylmethane family. Figure 5 shows the UV-vis spectra of EG (curve a), EG-ssDNA (curve b) and EG-dsDNA (curve c).…”

Section: Dna Binding Studiesmentioning

confidence: 99%

Developing a Nano‐Biosensor for DNA Hybridization Using a New Electroactive Label

et al. 2011

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Development of electrochemical DNA hybridization biosensors based on carbon paste electrode (CPE) and gold nanoparticle modified carbon paste electrode (NGMCPE) as transducers and ethyl green (EG) as a new electroactive label is described. Electrochemical impedance spectroscopy and cyclic voltammetry techniques were applied for the investigation and comparison of bare CPE and NGMCPE surfaces. Our voltammetric and spectroscopic studies showed gold nanoparticles are enable to facilitate electron transfer between the accumulated label on DNA probe modified electrode and electrode surface and enhance the electrical signals and lead to an improved detection limit. The immobilization of a 15-mer single strand oligonucleotide probe on the working electrodes and hybridization event between the probe and its complementary sequence as a target were investigated by differential pulse voltammetry (DPV) responses of the EG accumulated on the electrodes. The effects of some experimental variables on the performance of the biosensors were investigated and optimum conditions were suggested. The selectivity of the biosensors was studied using some non-complementary oligonucleotides. Finally the detection limits were calculated as 1.35×10-10 mol/L and 5.16×10 -11 mol/L on the CPE and NEGCPE, respectively. In addition, the biosensors exhibited a good selectivity, reproducibility and stability for the determination of DNA sequences.

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Synthesis and characterization of transition metal 2,6-pyridinedicarboxylic acid derivatives, interactions of Cu(II) and Ni(II) complexes with DNA in vitro

Cited by 60 publications

References 40 publications

Antibacterial properties of anthraquinones extracted from rhubarb against Aeromonas hydrophila

Antibacterial properties of anthraquinones extracted from rhubarb against Aeromonas hydrophila

Computational Insights into the Role of the Frontiers Orbital in the Chemistry of Tridentate Ligands

Developing a Nano‐Biosensor for DNA Hybridization Using a New Electroactive Label

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