Synthesis and characterization of Solanum nigrum-mediated silver nanoparticles and its protective effect on alloxan-induced diabetic rats

Abstract

Solanum nigrum, a medicinal plant, traditionally used in treating diabetes mellitus. In this study, we used the leaf extract of the plant to synthesize silver nanoparticles (AgNPs), as a proposition to treat alloxaninduced diabetic rats. The phytosynthesised AgNPs were analyzed using UV-visible and Fourier transform infra-red spectroscopy for their functional groups. Transmission electron microscopy revealed that, the synthesized particles are found to be 4-25 nm in size. Monodispersed and spherical nature of synthesized AgNPs were shown by scanning electron microscope and the presence of Ag in the AgNPs was confirmed by energy dispersive spectrum. The phytosynthesised AgNPs were evaluated for its antidiabetic activity in alloxan-induced diabetic rats. AgNPs-treated diabetic rats found to be significantly improved the dyslipidemic condition as seen in the diabetic control. Furthermore, it also reduced the blood glucose level over the period of treatment. The improvement in body weight was also found to be evidence for S. nigrum extract-mediated AgNPs as a potential antidiabetic agent against alloxaninduced diabetic rats.

Endophyte fungi, Cladosporium species-mediated synthesis of silver nanoparticles possessing in vitro antioxidant, anti-diabetic and anti-Alzheimer activity

“…The inhibition of these enzymes is concentration dependent. The CsAgNPs decreased the levels of enzymes, which are responsible for catalysing the hydrolysis of complex carbohydrates and increased the consumption rate of glucose reported by Rajaram et al [28], Abideen and Sankar [29] and Sengottaiyan et al [30]. A significant insulin sensitization activity was observed in 3T3L1 cell line followed by C2C12 and HepG2 cell lines at 3.34 mg of concentration of CsAgNPs (47%) (Figure 6).…”

Biosynthesis, characterisation and therapeutic applications of plant-mediated silver nanoparticles

“…75 The presence of secondary metabolites acting as reducing agents in the synthesis of AgNPs from Solanum nigrum was demonstrated by a peak at 2357 cm -1 , attributed to hydrogen-bonded OH stretching. 69 The FTIR spectra of AgNPs and Glycyrrhiza glabra root extract demonstrate that the potential biomolecules responsible for the synthesis and stabilization of AgNPs are flavonoids, terpenoids, thiamine and reducing sugars. 17…”

“…44 The XRD spectrum of AgNPs synthesized from Solanum nigrum showed 6 peaks at 2θ 10.00, 11.51, 12.00, 27.75, 32.15 and 45.94°, corresponding to six diffraction facets of silver. 69 In the case of AgNPs obtained from the Mussaenda erythrophylla leaf extract, the pattern showed characteristic Bragg reflections. The XRD spectrum shows only one clear signal at 2θ 38.50°, corresponding to (111) plane of face--centered cubic silver, demonstrating that the synthesized nanoparticles were highly pure.…”

“…14 The blue shift of the absorption peak located at 420 nm could be explained by the small particle size ≤ 25 nm. 68,69 2.2.3. Dynamic light scattering and determination of the zeta potential The particle size is determined by dynamic light scattering, a technique that characterizes the size of colloidal dispersion, by studying the illumination of a particle or molecule suspension in Brownian motion with a laser beam.…”

Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from <i>Physalis peruviana</i> L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats

“…Blood samples were collected from the tail vein of overnight fasted rats one day 0 (start of treatment), 7 th , 14 th , 21 st and 28 th day (end of treatment) [24] for determination of blood glucose levels using GlucoPlus® Active Glucometer.…”