Synthesis and characterization of silk fibroin microparticles for intra-articular drug delivery

Mwangi, Timothy K.; Bowles, Robby D.; Tainter, David M.; Bell, Richard D.; Kaplan, David L.; Setton, Lori A.

doi:10.1016/j.ijpharm.2015.02.059

Cited by 53 publications

(27 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, localization was improved with the novel treatment, thereby reducing the exposure of adjacent tissues to the fluorophore. All these findings suggest the need for further studies regarding this approach to intra-articular drug-delivery systems [133]. However, some problems arise from this work as the particles were not loaded with an actual drug or with non-hydrophobic molecules.…”

Section: Smart Drug-delivery Systems Not Involving Elrsmentioning

confidence: 95%

Elastin-Like Recombinamers As Smart Drug Delivery Systems

Arias

Santos

Ibáñez‐Fonseca

et al. 2018

CDT

View full text Add to dashboard Cite

show abstract

Section: Smart Drug-delivery Systems Not Involving Elrsmentioning

confidence: 95%

Elastin-Like Recombinamers As Smart Drug Delivery Systems

Arias

Santos

Ibáñez‐Fonseca

et al. 2018

CDT

View full text Add to dashboard Cite

show abstract

“…Silk fibroin from Bombyx mori , is a versatile material for drug delivery systems because of its unique combination of biocompatibility, controllable biodegradability, mechanical stability, and diverse processing windows [ 30 ]. The active amino groups of silk fibroin provide opportunities for chemical modification [ 31 ], and it is feasible to label this protein with quantum dots.…”

Section: Introductionmentioning

confidence: 99%

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

et al. 2020

View full text Add to dashboard Cite

When silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin particles and cell lines were investigated. Silk fibroin microparticles with dry size of 1.9 ± 0.4 μm (2.7 ± 0.3 μm in wet state) and silk fibroin nanoparticles with dry size of 51.5 ± 11.0 nm (174.8 ± 12.5 nm in wet state) were prepared by salting-out method and high-voltage electrospray method, respectively. CdSe/ZnS quantum dots were coupled to the surface of the micro/nanoparticles. Photostability observations indicated that the fluorescence stability of the quantum dots was much higher than that of fluorescein isothiocyanate. In vitro, microparticles and nanoparticles were co-cultured with human umbilical vein endothelial cells EA.hy 926 and cervical cancer cells HeLa, respectively. The fluorescence test and cell viability showed that the EA.hy926 cells tended to be adhered to the microparticle surfaces and the cell proliferation was significantly promoted, while the nanoparticles were more likely to be internalized in HeLa cells and the cell proliferation was notably inhibited. Our findings might provide useful information concerning effective drug delivery that microparticles may be preferred if the drugs need to be delivered to normal cell surface, while nanoparticles may be preferred if the drugs need to be transmitted in tumor cells.

show abstract

“…Silk fibroin (SF) is a natural protein, derived from Bombyx mori cocoons, which has been used for many years in biomedical fields thanks to its biocompatible and biodegradable properties [16,17,18,19]. Silk fibroin was studied as drug carriers; in particular, fibroin-based microparticles were proposed for wound healing, intra-articular delivery, and parathyroid hormone delivery applications [20,21,22,23]. Silk fibroin was also proposed for the production of nanoparticulate systems able to carry small molecules, protein, gene and anti-cancer drugs [7,24,25].…”

Section: Introductionmentioning

confidence: 99%

Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs

et al. 2019

View full text Add to dashboard Cite

The aim of this work was to load an anticancer drug, paclitaxel (PTX), on Silk Fibroin Nanoparticles (SFNs) by using an exogenous approach. SFNs were produced, freeze-dried and then loaded with PTX. An exogenous method allowed us to reduce both drug loss and environmental impact. In order to quantify PTX loaded in SFNs, a simple and reliable method using reversed phase liquid chromatography coupled to tandem mass spectrometry (rp-UHPLC-MS/MS) was developed. This methodology was validated by the determination of spiked QC samples in three consecutive days. Good accuracy and precision of the method were obtained, while the intra-day and inter-day precisions were less than 10.3%. For PTX, the limit of quantitation (LOQ) was 5.0 ng/mL. Recovery from the matrix (SFNs-PTX pellets) was calculated (81.2% at LOQ value) as PTX was entrapped in a new matrix like the polymer silk fibroin-based. This method was successfully applied to determine the encapsulation efficiency (1.00 ± 0.19%) and the nanoparticle loading (0.12 ± 0.02% w/w). The in vitro anticancer activity of SFNs-PTX was tested against CFPAC-1 cancer cells; results demonstrated a very high cytotoxic activity of SFNs-PTX, with a dose dependent inhibition of CFPAC-1 proliferation, confirmed by the IC50 value of 3450 ± 750 ng/mL.

show abstract

Synthesis and characterization of silk fibroin microparticles for intra-articular drug delivery

Cited by 53 publications

References 45 publications

Elastin-Like Recombinamers As Smart Drug Delivery Systems

Elastin-Like Recombinamers As Smart Drug Delivery Systems

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs

Contact Info

Product

Resources

About