Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes

Sun, Honghao; Scharff-Poulsen, Anne Marie; Gu, Hongchen; Almdal, Kristoffer

doi:10.1021/cm052750j

Cited by 133 publications

(138 citation statements)

References 22 publications

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“…For example, the ratio of two fluorescent peaks is used instead of the absolute intensity of one peak. The sensors therefore typically contain a reference dye; the advantage of this approach is that factors such as excitation source fluctuations and sensor concentration will not affect the ratio between the fluorescence intensities of the indicator and the reference dye Buck et al, 2004;Cywinski et al, 2009;Doussineau et al, 2009;Frigoli et al, 2009;Sun et al, 2006).…”

Section: Optical Detection Principlesmentioning

confidence: 99%

“…In any case, the optical properties that can be controlled at the nanoscale are of great interest in the field of optical sensor designing (Borisov & Klimant, 2008). Some optical chemical NSs rely on quantum dots (Asefa et al 2009), metal beads (Shtykov & Rusanova, 2008) and other materials; however, most of them make use of indicators embedded in polymer beads (Lapresta et al, 2009;Zenkl & Klimant, 2009) and sol-gels Hun & Zhang, 2007;Sun et al, 2006).…”

Section: Effect Of Nanodimensions On Sensor Characteristicsmentioning

confidence: 99%

“…Both physical (Borisov et al, 2009) entrapment and covalent (Sun et al, 2006) coupling are used. Physical entrapment of an indicator in NPs is preferred because of its simplicity and reproducibility.…”

Section: Polymer-based Nanoparticlesmentioning

confidence: 99%

“…In the field of biology and medicine, sensors able to monitor pH in real time are in great demand, as they can be used for the general understanding of biological processes and for biomedical diagnostics (Doussineau et al, 2009). Recently, a lot of work has been done on optical pH-sensitive micro- (Borisov et al, 2008a;Doussineau et al, 2009;Schulz et al, 2009 ;Hornig et al, 2008) and nano-sensors (Allard & Larpent, 2008;Burns et al, 2006;Gao et al, 2005;Gao et al, 2007;Kim et al, 2006;Peng et al, 2007;Sun et al, 2006;). Here, the latter will be discussed, since they are less than 100 nm in size.…”

Section: Nanosensors For Phmentioning

confidence: 99%

“…Several sol-gel pH NSs were prepared using a microemulsion procedure (Allard & Larpent, 2008;Gao et al, 2005;Gao et al, 2007;Kim et al, 2006;Peng et al, 2007;Sun et al, 2006) and one by Stöber (Burns et al, 2006). Gao et al, 2007, designed pH sensors using the doping technique for the immobilization of indicators in sol-gel NPs.…”

Section: Advances In Chemical Sensorsmentioning

confidence: 99%

See 4 more Smart Citations

Optical Chemical Sensors:Design and Applications

Lobnik¹,

Turel²,

Urek³

2012

Advances in Chemical Sensors

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Section: Optical Detection Principlesmentioning

confidence: 99%

Section: Effect Of Nanodimensions On Sensor Characteristicsmentioning

confidence: 99%

Section: Polymer-based Nanoparticlesmentioning

confidence: 99%

Section: Nanosensors For Phmentioning

confidence: 99%

Section: Advances In Chemical Sensorsmentioning

confidence: 99%

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Optical Chemical Sensors:Design and Applications

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Turel²,

Urek³

2012

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Delivery of Nanonsensors to Measure the Intracellular Environment

Coupland

Aylott

2010

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Fluorescence Quenching upon Binding of Copper Ions in Dye‐Doped and Ligand‐Capped Polymer Nanoparticles: A Simple Way to Probe the Dye Accessibility in Nano‐Sized Templates

Gouanvé

Schuster

Allard

et al. 2007

Adv Funct Materials

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The synthesis and properties of well‐defined core–shell type fluorescent metal‐chelating polymer nanoparticles NP, in the 15 nm diameter range, with a fluorophore (9,10‐diphenylanthracene: DPA) entrapped in the particle core and a selective ligand (1,4,8,11‐tetraazacyclotetradecane: Cyclam), grafted onto the surface are presented. NPs with different number of dye‐per‐particle are readily obtained by entrapment of the fluorophore within the polymer core. The ligand‐coated NPs exhibit a high affinity for Cu2+ ions in aqueous solution and quenching of the DPA fluorescence is observed upon binding of copper. The quenching of fluorescence arises through energy transfer (FRET) from the dye to the copper‐cyclam complexes that form at the NP surface with an operating distance (d) in the 2 nm range. A simple core–shell model accounts for the steady‐state and time‐resolved fluorescence titration experiments: dye molecules located in the outer sphere (thickness d) of the NPs are quenched while the fluorescence of dyes embedded more deeply is not affected by the binding of copper ions. The observed high quenching efficiency (60–65 %), which is tightly correlated to the volumic and microstructural features of the NPs, shed light on the enhanced accessibility inherent in nano‐sized templates. The response towards different metal ions was investigated and this confirmed the selectivity of the nanoparticle template‐assembled sensor for cupric ions.

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Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes

Cited by 133 publications

References 22 publications

Optical Chemical Sensors:Design and Applications

Optical Chemical Sensors:Design and Applications

Delivery of Nanonsensors to Measure the Intracellular Environment

Fluorescence Quenching upon Binding of Copper Ions in Dye‐Doped and Ligand‐Capped Polymer Nanoparticles: A Simple Way to Probe the Dye Accessibility in Nano‐Sized Templates

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