Synthesis and Characterization of PLGA Shell Microcapsules Containing Aqueous Cores Prepared by Internal Phase Separation

Abulateefeh, Samer R.; Alkilany, Alaaldin M.

doi:10.1208/s12249-015-0413-y

Cited by 32 publications

(13 citation statements)

References 29 publications

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“…Therefore, developing appropriate drug delivery systems with tunable sequential drug release ability and good biocompatibility is of vital importance to obtain more effective therapeutic effects. Microspheres (MS) and microcapsules are widely studied in tissue engineering and pharmaceutics, which can encapsulate and protect various drugs or cytokines to enable long-term and sustained release, so that these drugs can exert functions on cells and tissues in a controlled manner [ 1–3 ].…”

Section: Introductionmentioning

confidence: 99%

Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release

Liu

Zheng

et al. 2020

Regenerative Biomaterials

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Sequential administration and controlled release of different drugs are of vital importance for regulating cellular behaviors and tissue regeneration, which usually demands appropriate carriers like microspheres (MS) to control drugs releases. Electrospray has been proven an effective technique to prepare MS with uniform particle size and high drug-loading rate. In this study, we applied electrospray to simply and hierarchically fabricate sphere-in-sphere composite microspheres, with smaller poly(lactic-co-glycolic acid) MS (∼8–10 μm in diameter) embedded in a larger chitosan MS (∼250–300 μm in diameter). The scanning electron microscopy images revealed highly uniform MS that can be accurately controlled by adjusting the nozzle diameter or voltage. Two kinds of model drugs, bovine serum albumin and chlorhexidine acetate, were encapsulated in the microspheres. The fluorescence-labeled rhodamine-fluoresceine isothiocyanate (Rho-FITC) and ultraviolet (UV) spectrophotometry results suggested that loaded drugs got excellent distribution in microspheres, as well as sustained, slow release in vitro. In addition, far-UV circular dichroism and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) results indicated original secondary structure and molecular weight of drugs after electrospraying. Generally speaking, our research proposed a modified hierarchically electrospraying technique to prepare sphere-in-sphere composite MS with two different drugs loaded, which could be applied in sequential, multi-modality therapy.

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Section: Introductionmentioning

confidence: 99%

Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release

Liu

Zheng

et al. 2020

Regenerative Biomaterials

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“…The drug loading and entrapment efficiency are crucial if the microspheres are to be useful clinically. The processing parameters used to make the spheres have a significant influence on both of these, and the optimum conditions have been found to vary with the drug used (Nan et al., 2014 ; Alcalá-Alcalá et al., 2015 ; Abulateefeh & Alkilany, 2016 ; Rafiei & Haddadi, 2017 ). A range of different conditions (inner water phase/oil phase and oil phase/external water phase ratios; PVA concentration; ultrasound time and speed) were used to prepare PLGA microspheres and these were coated with eight alternating layers of PVA and p(AAPBA-co-NVCL).…”

Section: Resultsmentioning

confidence: 99%

Insulin-loaded PLGA microspheres for glucose-responsive release

Williams

et al. 2017

Drug Delivery

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Porous poly(lactic-co-glycolic acid) (PLGA) microspheres were prepared, loaded with insulin, and then coated in poly(vinyl alcohol) (PVA) and a novel boronic acid-containing copolymer [poly(acrylamide phenyl boronic acid-co-N-vinylcaprolactam); p(AAPBA-co-NVCL)]. Multilayer microspheres were generated using a layer-by-layer approach depositing alternating coats of PVA and p(AAPBA-co-NVCL) on the PLGA surface, with the optimal system found to be that with eight alternating layers of each coating. The resultant material comprised spherical particles with a porous PLGA core and the pores covered in the coating layers. Insulin could successfully be loaded into the particles, with loading capacity and encapsulation efficiencies reaching 2.83 ± 0.15 and 82.6 ± 5.1% respectively, and was found to be present in the amorphous form. The insulin-loaded microspheres could regulate drug release in response to a changing concentration of glucose. In vitro and in vivo toxicology tests demonstrated that they are safe and have high biocompatibility. Using the multilayer microspheres to treat diabetic mice, we found they can effectively control blood sugar levels over at least 18 days, retaining their glucose-sensitive properties during this time. Therefore, the novel multilayer microspheres developed in this work have significant potential as smart drug-delivery systems for the treatment of diabetes. ARTICLE HISTORY

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“…Compared with the traditional PLGA microspheres, the preparation of PLGA microcapsules with water-phase nuclei has higher technical difficulties [ 25 ]. PLGA microspheres have shortcomings in drug loading, encapsulation efficiency, and drug release, especially for water-soluble drugs and hydrophilic macromolecules [ 26 – 28 ]. In this paper, ET-PLGA microcapsules were prepared by double emulsion—solvent evaporation (W 1 /O/W 2 ).…”

Section: Discussionmentioning

confidence: 99%

Application of Implantable Polylactic-Co-Glycolic Acid Microcapsule in Repairing Alveolar Bone Defects

Jiang

Xiao

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Alveolar bone defects (ABDs) were a perennial problem, especially in the aged. Bisphosphonates, especially etidronate sodium (ET), were frequently used in clinical treatment of ABD. However, the oral administration of ET had poor absorption (<1%). Therefore, optimization of a suitable dosage form substituted with ET to locally repair the ABD was a straightforward approach. Polylactide-co-glycolide (PLGA) is a biodegradable material and had been used in locally implanted medical devices. Therefore, an ET-PLGA microcapsule may help local delivery and prolong the activity of healing ABD. In this paper, a preparation method of ET-PLGA microcapsule was optimized by the single-factor investigation and response surface method. Subsequently, the rat ABD model was used to evaluate the enhancement effect of these microcapsules. Finally, the optimum parameters were determined as follows: 40% dichloromethane, 160 mg/mL PLGA, 10% internal aqua/oil phase, 4% PVA, and emulsifying for 10 min. These microcapsules were spherical in shape and fairly monodisperse in a particle size of 27,51 μm (PDI = 0.3), encapsulation rate 96.6%, and drug loading 4.58%. Compared with the ET groups, the total healing volume of ABD in ET-PLGA groups was significantly increased P < 0.05 . ET-PLGA microcapsules significantly enhanced the effect of ET on ABD. This study provided important technical support for the treatment of ABD with bisphosphonates by local administration. This paper has an exploratory significance for the development of water-soluble bioactive components with low bioavailability for ABD.

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Synthesis and Characterization of PLGA Shell Microcapsules Containing Aqueous Cores Prepared by Internal Phase Separation

Cited by 32 publications

References 29 publications

Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release

Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release

Insulin-loaded PLGA microspheres for glucose-responsive release

Application of Implantable Polylactic-Co-Glycolic Acid Microcapsule in Repairing Alveolar Bone Defects

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