Synthesis and Characterization of Nanohydroxyapatite-Gelatin Composite with Streptomycin as Antituberculosis Injectable Bone Substitute

Hikmawati, Dyah; Maulida, Hendita Nur; Putra, Alfian Pramudita; Budiatin, Aniek Setiya; Syahrom, Ardiyansyah

doi:10.1155/2019/7179243

Cited by 18 publications

(21 citation statements)

References 21 publications

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“…The process of healing fracture and repairing bone tissue involves an initial anabolic phase characterized by an increase in tissue volume associated with de novo recruitment and differentiation of stem cell that form bone and Callus cartilage tissue blood vessel around the fracture area. This process will occur from the periphery to the center of the fracture to the end of the cartilage network (Einhorn 2010, Einhorn & Gesternfeld 2015, Hikmawati et al 2019.…”

Section: Discussionmentioning

confidence: 99%

Acceleration of Bone Fracture Healing through the Use of Natural Bovine Hydroxyapatite Implant on Bone Defect Animal Model

Khotib

Lasandara

Samirah

et al. 2019

FMI

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Bone is an important organ for supports the body that stores reserve of calcium, phosphorus, and other minerals. In fracture conditions where bleeding, soft tissue edema, nerve damage, and blood vessels around the bone damage happen, they can cause the mobilization of these minerals in the surrounding tissue. One of the efforts made in the treatment of these fractures is reconnection, in which it works by filling of bone defect with a matrix and administration of anti-infection. Biomaterial filling in defective bone is thought to accelerate the healing process of bone fracture and prevent osteomyelitis. For this reason, this study evaluates the acceleration of bone fracture healing using natural hydroxyapatite (NHA) bone filler in rabbits with bone defect model. Fracture modeling was performed by surgical technique and drilling of bones with a 4.2 mm diameter to form a defect in the rabbit femur. Bone implant contained bovine hydroxyapatite-gelatin-glutaraldehyde (BHA implant) or bovine hydroxyapatite-gelatin-glutaraldehyde-gentamicin (BHA-GEN implant) that was inserted in bone defects. 27 rabbits were divided into 3 groups: the control group who had bone defect, the bone defect group was given BHA implant and the bone defect group was given BHA-GEN implant. Observation of osteoclast, osteoblast, osteocyte, BALP level, and bone morphological integrity was carried out on the 14th, 28th, and 42nd days after surgery. Histological observation of rabbit femur showed a significant difference on the number of osteoclast, osteoblast and osteocyte in all three groups. The BALP level also showed a significant difference in the group given the natural BHA bone implant compared to the control group on day 14 (p = 0.0361). Based on the result of the X-ray, there was also a better integration of rabbit femur bone in groups with the use of BHA or BHA-GEN bone implant. Thus, it can be concluded that the use of a natural BHA implant can accelerate the process of bone repair in the fracture of rabbit femur. In addition, BHA implants were compatible as a matrix for supporting the bone cell growth.

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Section: Discussionmentioning

confidence: 99%

Acceleration of Bone Fracture Healing through the Use of Natural Bovine Hydroxyapatite Implant on Bone Defect Animal Model

Khotib

Lasandara

Samirah

et al. 2019

FMI

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“…Each experiment was repeated three times. This methodology was based on the methodology employed by Dyah Hikmawati and co-authors [30].…”

Section: Injectabilitymentioning

confidence: 99%

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix

Medrano-David

Lopera

Londoño

et al. 2021

JFB

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The occurrence of bone-related disorders and diseases has dramatically increased in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision. In addition, these particles are susceptible to migration to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill a bone defect. The aim of this research was to develop a DBM carrier with such viscoelastic properties in order to obtain an injectable bone substitute (IBS). The developed DBM carrier consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by an injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results showed that the developed material has great potential to be used in bone tissue regeneration.

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“…Each experiment was repeated three times. This methodology was based on the methodology employed by Dyah Hikmawati and co-authors [29].…”

Section: Injectabilitymentioning

confidence: 99%

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/borax /CaCO3 and Demineralized Bone Matrix

Medrano-David¹,

Lopera²,

Londoño³

et al. 2021

Preprint

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The occurrence of bone-related disorders and diseases has increased dramatically in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision, in addition, these particles are susceptible to migrate to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill the bone defect. The aim of this research is to develop a DBM carrier with such viscoelastic properties to obtain an injectable bone substitute (IBS). The DBM carrier developed consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by the injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results show that the developed material has great potential to be used in bone tissue regeneration

show abstract

Synthesis and Characterization of Nanohydroxyapatite-Gelatin Composite with Streptomycin as Antituberculosis Injectable Bone Substitute

Cited by 18 publications

References 21 publications

Acceleration of Bone Fracture Healing through the Use of Natural Bovine Hydroxyapatite Implant on Bone Defect Animal Model

Acceleration of Bone Fracture Healing through the Use of Natural Bovine Hydroxyapatite Implant on Bone Defect Animal Model

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix

Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/borax /CaCO3 and Demineralized Bone Matrix

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