Synthesis and characterization of multiwalled carbon nanotubes-protoporphyrin IX composites using acid functionalized or nitrogen doped carbon nanotubes

Tostado-Plascencia, M.M.; Sánchez, Marciano; Zamudio‐Ojeda, Adalberto; Suárez-Gómez, A.; Castañeda-Valderrama, R.; Carreón-Álvarez, M.A.; Morán-Lázaro, Juan Pablo; Sebastian, P.J.; Salgado‐Tránsito, Iván

doi:10.1016/j.diamond.2016.09.030

Cited by 4 publications

(1 citation statement)

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“…The majority of the molecular mass is based on the pyrrole ring, which begins thermal decomposition around 360–450°C as evidenced by TGA analysis. Although there is a slight mass reduction from 26–360°C, this has been mainly linked to the breaking of reactive bonds outside the ring [41]. Therefore, it is highly likely host-guest embedding does not significantly alter PpIX structure and functionality during microwave-assisted synthesis.…”

Section: Discussionmentioning

confidence: 99%

Carbon dot-protoporphyrin IX conjugates for improved drug delivery and bioimaging

2019

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Photodynamic therapy (PDT) uses photosensitisers such as protoporphyrin IX (PpIX) to target tumours via the release of toxic singlet oxygen when irradiated. The effectivity of the treatment is limited by the innate properties of the photosensitizers; they typically exhibit inefficient accumulation in target tissue and high dark toxicity. Carbon dots (CDs) are biocompatible fluorescent nanoparticles which can improve PpIX cellular uptake and solubility. In this work, we present conjugates synthesised by host-guest encapsulation (PpIX@CD) and amide cross-linking (PpIX-CD). Characterization demonstrated conjugates have a loading efficiency of 34–48% and similar singlet oxygen production to PpIX. PpIX-containing CDs showed a 2.2 to 3.7-fold decrease in dark toxicity. PpIX-CD and PpIX@CD showed equivalent light-induced toxicity to PpIX in concentrations >1 μg/ml, leading to a 3.2 to 4.1-fold increase in photo-toxicity index (PI). The less soluble fraction of cross-linked conjugates (PpIX-CD)p did not show significant difference from PpIX. Confocal light scanning microscopy demonstrated rapid intracellular uptake and accumulation of conjugates. Our results demonstrate the variations between cross-linking strategies in CD-based conjugates, highlighting their potential as carriers in drug delivery and bioimaging applications.

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Section: Discussionmentioning

confidence: 99%