Synthesis and characterization of gold-conjugated Backhousia citriodora nanoparticles and their anticancer activity against MCF-7 breast and HepG2 liver cancer cell lines

Khandanlou, Roshanak; Murthy, Vinuthaa; Saranath, Dhananjaya; Damani, Hetal

doi:10.1007/s10853-017-1756-4

Cited by 41 publications

(8 citation statements)

References 49 publications

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“…Metals like gold revealed anticarcinogenic property by the disorientation of cellular structure triggered shrinkage of the membrane and enhanced the peptide cellulolytic activity serving synergistic activity on MCF-7 cell line. [43,44] Under alkaline conditions, HAuCl 4 was reduced by peptide's tyrosine phenolic group and thiols SH group to help in the attachment and penetration of NPY in the cancerous cell and influenced phosphorylation causing apoptosis. The positive surface charge of formulation NCN on contact with negatively charged cancerous cell showed the disruption of cellular structure and cell membrane by the presence of electrostatic charges to facilitate NPY penetration across the cytosol.…”

Section: Discussionmentioning

confidence: 99%

Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line

Desai

Shende

2021

Biotechnology Journal

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Gold nanoclusters (AuNCs) are potential carrier system for bioactive like proteins and peptides used in various therapeutics against various ailments. Neuropeptide Y (NPY) is consists of 36 amino acids used to treat depression, obesity, epilepsy, and so on.but possess instability at higher temperatures causing its limited usage. The present study focused on the NPY-decorated AuNCs prepared using desolvation reduction technique and optimized through randomized hybrid design. ATR-FTIR, 1 H NMR and CD spectroscopic studies confirmed the AuNCs structure interaction with NPY. The optimized NPY-decorated AuNCs possessed 85.6 ± 2.08% of entrapment efficiency with 85.32 ± 7.55% of NPY release for 24 h. It displayed dose-dependent cell cytotoxicity, IC 50 value of 0.7 ± 0.05 μg mL -1 and apoptosis of 68.48 ± 7.35% with controlled cell migration causing G0G1 cell arrest by penetrating cancer cell membrane on MCF-7 cell line. Furthermore, the AuNCs caused surface disruption of the cancerous cell further interrupting the protein synthesis by MAPK pathway leading to cell death. The AuNCs were stable for 3 months at 25 ± 2 • C due to steric hindrance. Hence, NPY-decorated AuNCs were found to be effective on MCF-7 cell line with a significant anti-apoptotic effect, further emerging as a novel therapeutic delivery system in the management of breast cancer.

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Section: Discussionmentioning

confidence: 99%

Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line

Desai

Shende

2021

Biotechnology Journal

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“…Additional advantage of this method is that the extract itself acts as a stabilizer and no additional stabilizers are required. The extracts of Aspergillus fischeri [ 19 ], Epicoccum nigrum [ 20 ], bark of Mimusops elengi [ 21 ], leaf of Chrysophyllum cainito (Star apple) [ 22 ], bark of Abroma augusta Linn, [ 23 ] Backhousia citriodora [ 24 ], Breynia rhamnoides [ 25 ], Piper betle [ 26 ], pear extract [ 27 ] etc. have been utilized for the synthesis of AuNPs.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of Nerium oleander-conjugated gold nanoparticles and study of its in vitro anticancer activity on MCF-7 cell lines and catalytic activity

et al. 2018

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The phytochemicals present in the stem bark extract of Nerium oleander (commonly known as Karabi) have been utilized for the green synthesis of stable gold-conjugated nanoparticles at room temperature under very mild conditions. The green synthesized gold-conjugated nanoparticles were characterized by surface plasmon resonance spectroscopy, High resolution transmission electron microscopy, X-ray diffraction studies and dynamic light scattering. A mechanism for the synthesis and stabilization of gold-conjugated nanoparticles (AuNPs) has been proposed. Anticancer activity of the stabilized AuNPs studied against MCF-7 breast cancer cell line revealed that the stabilized AuNPs were highly effective for the apoptosis of cancer cells selectively. The antioxidant activity of the stem bark extract of Nerium oleander has also been studied against a long lived 2,2-diphenylpicrylhydrazyl radical at room temperature. Moreover, the utilization of the stabilized AuNPs as a catalyst has also been demonstrated. Electronic supplementary materialThe online version of this article (10.1186/s40580-018-0142-5) contains supplementary material, which is available to authorized users.

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“…Over the last two decades, Au NPs have been mainly used as biosensors and as contrast agents in X-ray imaging and computed tomography (Kobayashi et al 2016 ), being applied in biological and biomedical research, especially in cancer diagnosis (Chuang et al 2018 ; Kim et al 2018 ; Sugumaran et al 2018 ) and therapy (Khandanlou et al 2018 ; Zhu et al 2018 ), biosensing (Baetsen-Young et al 2018 ; Chen et al 2009 ), virus detection, and regulation of cell function (Bodelon et al 2017 ; Giljohann et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles

et al. 2018

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Hydrophilic and homogeneous sub-10 nm blue light-emitting gold nanoparticles (NPs) functionalized with different capping agents have been prepared by simple chemical routes. Structure, average, size, and surface characteristics of these NPs have been widely studied, and the stability of colloidal NP solutions at different pH values has been evaluated. Au NPs show blue PL emission, particularly in the GSH capped NPs, in which the thiol-metal core transference transitions considerably enhance the fluorescent emission. The influence of capping agent and NP size on cytotoxicity and on the fluorescent emission are analyzed and discussed in order to obtain Au NPs with suitable features for biomedical applications. Cytotoxicity of different types of gold NPs has been determined using NPs at high concentrations in both tumor cell lines and primary cells. All NPs used show high biocompatibility with low cytotoxicity even at high concentration, while Au-GSH NPs decrease viability and proliferation of both a tumor cell line and primary lymphocytes.Electronic supplementary materialThe online version of this article (10.1007/s11051-018-4406-0) contains supplementary material, which is available to authorized users.

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Synthesis and characterization of gold-conjugated Backhousia citriodora nanoparticles and their anticancer activity against MCF-7 breast and HepG2 liver cancer cell lines

Cited by 41 publications

References 49 publications

Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line

Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line

Green synthesis of Nerium oleander-conjugated gold nanoparticles and study of its in vitro anticancer activity on MCF-7 cell lines and catalytic activity

Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles

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