Synthesis and characterization of efficient chemical nucleases: A joint refinement from XRD, antimicrobial screening, DNA binding and cleavage investigations

Raman, Natarajan; Mahalakshmi, Rajkumar; Packiaraj, Muthusamy

doi:10.1016/j.inoche.2014.07.011

Cited by 13 publications

(8 citation statements)

References 26 publications

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“…The percentage hypochromicity ( H (%) = [( A free − A bound )/ A free ] × 100) of ILCT bands of complexes I to VII upon binding to DNA are found to be in the range 18.68–18.16 ± 0.90% (supporting information). Complex I has highest percentage hypochromicity (18.68 ± 0.82%) . The observed hypochromism could be attributed to stacking interaction between the aromatic chromophores of the complexes and DNA base pairs possible with the intercalative binding mode, while the red shift is evidence of the stabilization of the HS DNA duplex.…”

Section: Resultsmentioning

confidence: 90%

Synthesis, spectral investigation and development of tetrahedral copper(II) complexes as artificial metallonucleases and antimalarial agents

Mehta

Gajera

Patel

et al. 2015

Applied Organom Chemis

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*Seven new copper(II) complexes of type [Cu(A)(L)]ÁH 2 O (A = sparfloxacin, ciprofloxacin, levofloxacin, gatifloxacin, pefloxacin, ofloxacin, norfloxacin; L = 5-[(3-chlorophenyl)diazenyl]-4-hydroxy-1,3-thiazole-2(3H)-thione) were synthesized and characterized using elemental and thermogravimetric analyses, and electronic, electron paramagnetic resonance (EPR), Fourier transform infrared and liquid chromatography-mass spectroscopies. Tetrahedral geometry around copper is assigned in all complexes using EPR and electronic spectral analyses. All complexes were investigated for their interaction with herring sperm DNA utilizing absorption titration (K b = 1.27-3.13 × 10 5 M À1) and hydrodynamic volume measurement studies. The studies suggest the classical intercalative mode of DNA binding. The cleavage reaction on pUC19 DNA was monitored by agarose gel electrophoresis. The results indicate that the Cu(II) complexes can more effectively promote the cleavage of plasmid DNA. The superoxide dismutase mimic activity of the complexes was evaluated by nitroblue tetrazolium assay, and the complexes catalysed the dismutation of superoxide at pH = 7.8 with IC 50 values in the range 0.597-0.900 μM. The complexes were screened for their in vitro antibacterial activity against five pathogenic bacteria. All the complexes are good cytotoxic agents and show LC 50 values ranging from 5.559 to 11.912 μg ml À1. All newly synthesized Cu(II) complexes were also evaluated for their in vitro antimalarial activity against Plasmodium falciparum strain (IC 50 = 0.62-2.0 μg ml À1).

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Section: Resultsmentioning

confidence: 90%

Synthesis, spectral investigation and development of tetrahedral copper(II) complexes as artificial metallonucleases and antimalarial agents

Mehta

Gajera

Patel

et al. 2015

Applied Organom Chemis

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“…which were significantly higher than those of complex 2 (14 and 29%, respectively). The observed hypochromism and bathochromism provided clear evidences that complexes 1-2 interacted with CT-DNA by the intercalative binding mode [19]. Furthermore, it was noted that hyperchromism was attributed to the damage of DNA double helix, which indicating the existence of partial or nonintercalative binding modes [20].…”

Section: Changes In Uv-vis Spectra Of Complexes 1-2mentioning

confidence: 85%

Diorganotin (IV) complexes with 4-nitro-N-phthaloyl-glycine: Synthesis, characterization, antitumor activity and DNA-binding studies

Yan

Zhang

Tang

et al. 2015

Biomedicine & Pharmacotherapy

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“…Viscosity measurements to examine the change in hydrodynamic chain length may be used to monitor such DNA structural changes by the length changes of rod-like DNA [16]. The different concentrations of fixed solution of complexes 1-7 and DNA in phosphate buffer media are used, i.e.…”

Section: Hydrodynamic Chain Length Studymentioning

confidence: 99%

Copper(II) Complexes with N,O-Donor Ligands and Ofloxacin Drug as Antibacterial, DNA Interacting, Cytotoxic and SOD Mimic Agent

et al. 2015

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The N,O-donor bidentate ligands (L 1 -L 7 ) derived from the reaction between chalcones and pyridinium salt of 2-acetyl furan were synthesized and characterized by IR and NMR spectroscopic techniques. Their complexes [1][2][3][4][5][6][7] of Cu(II) were synthesized and characterized by elemental analysis, magnetic measurements, TG analyses, IR and mass spectroscopy. Synthesized complexes were carried out for their biological elucidation using different biological experiments like minimum inhibitory concentration, DNA binding and cleavage study, cytotoxicity, and antiradical activity. Efficient cleavage of pUC19 DNA was observed for all the test complexes than the reference drug. Graphical Abstract Increase in DNA chain length and hence the relative viscosity as the complexes binds to DNA via intercalative mode and involves a strong stacking interaction between an aromatic chromophore and the DNA base pair.Electronic supplementary material The online version of this article

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Synthesis and characterization of efficient chemical nucleases: A joint refinement from XRD, antimicrobial screening, DNA binding and cleavage investigations

Cited by 13 publications

References 26 publications

Synthesis, spectral investigation and development of tetrahedral copper(II) complexes as artificial metallonucleases and antimalarial agents

Synthesis, spectral investigation and development of tetrahedral copper(II) complexes as artificial metallonucleases and antimalarial agents

Diorganotin (IV) complexes with 4-nitro-N-phthaloyl-glycine: Synthesis, characterization, antitumor activity and DNA-binding studies

Copper(II) Complexes with N,O-Donor Ligands and Ofloxacin Drug as Antibacterial, DNA Interacting, Cytotoxic and SOD Mimic Agent

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