Synthesis and characterization of dextran hydrogels prepared with chlor‐ and nitrogen‐containing crosslinkers

İmren, Dilek; Gümüşderelı́oğlu, Menemşe; Güner, Ali̇

doi:10.1002/app.24670

Cited by 22 publications

(15 citation statements)

References 25 publications

(21 reference statements)

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“…It is known that in aqueous alkaline media, the dextran hydrogel is formed under dextran cross-linking by ECH or other cross-linkers. [15,16] Several studies established that chemical cross-linking of some water-soluble polysaccharides in diluted aqueous solutions (in order to prevent macroscopic gelation) leads to formation of cross-linked hydrogel particles with tunable sizes from sub-micrometers to tens of nanometers. [17] Here, by the reducing the concentration of dextran to 1 wt% (that is insufficient to form entire gel) the nanoparticles of cross-linked dextran were obtained.…”

Section: Resultsmentioning

confidence: 99%

Dextran Nanoparticles Cross‐Linked in Aqueous and Aqueous/Alcoholic Media

Iakobson

Добродумов

Saprykina

et al. 2017

Macro Chemistry & Physics

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The attractiveness of the dextran nanoparticle application in medicine and pharmacology results from biocompatibility, biodegradability, and nontoxicity of the polymer. However, it still remains a challenge to prepare such nanoparticles in an aqueous medium due to dextran hydrophilicity. Here, the technique of dextran nanoparticle synthesis with the average diameter of 140–450 nm in aqueous and aqueous/ethanolic media is described. The chemical structure of cross‐linker used for the dextran nanoparticle stabilization is demonstrated to influence the dextran nanoparticle diameter, size distribution, and ζ‐potential. As shown the regulation of the nanoparticle diameter and ζ‐potential is possible through the surfactants and/or dextran nonsolvent (ethanol) addition into the reaction medium. Due to the additional steric stabilization, the dextran nanoparticles of minimum diameter are formed in the presence of ionic surfactants in the aqueous/ethanolic medium. The nanoparticle shape and diameter are analyzed by dynamic light scattering, transmission electron microscopy, and scanning electron microscopy; the composition is investigated by 1H NMR spectroscopy.

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Section: Resultsmentioning

confidence: 99%

Dextran Nanoparticles Cross‐Linked in Aqueous and Aqueous/Alcoholic Media

Iakobson

Добродумов

Saprykina

et al. 2017

Macro Chemistry & Physics

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“…As depicted in Figure 6, the particles exhibited no toxicity (at least 100% viability) after 24 hours. Dextran is a hydrophilic, water-soluble polymer which is inert in biological systems, therefore decreasing protein adsorption to the nanoparticle surface which would lead to toxicity [23]. After 48 hours, the particles were still mostly non-toxic at these concentrations, but there was a significant difference between the control and the CREKA-IONP at 100, 350, and 500 μg Fe/mL as determined via a 1-way ANOVA test.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and characterization of CREKA-conjugated iron oxide nanoparticles for hyperthermia applications

et al. 2014

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One of the current challenges in the systemic delivery of nanoparticles in cancer therapy applications is the lack of effective tumor localization. Iron oxide nanoparticles coated with crosslinked dextran were functionalized with the tumor homing peptide CREKA, which binds to fibrinogen complexes in the extracellular matrix of tumors. This allows for the homing of these nanoparticles to tumor tissue. The iron oxide nanoparticle core allows for particle heating upon exposure to an alternating magnetic field (AMF) while the dextran coating stabilizes the particles in suspension and decreases the cytotoxicity of the system. Magnetically mediated hyperthermia (MMH) allows for the heating of tumor tissue to increase the efficacy of traditional cancer treatments using the iron oxide nanoparticles. While MMH provides the opportunity for localized heating, this method is currently limited by the lack of particle penetration into tumor tissue, even after effective targeted delivery to the tumor site. The CREKA-conjugated nanoparticles presented were characterized for their size, stability, heating capabilities and biocompatibility. The particles had a hydrated diameter of 52 nm, were stable in PBS and media with 10% v/v FBS over at least twelve hours, and generated enough heat to raise solution temperatures well into the hyperthermia range (41 – 45 °C) when exposed to an AMF due to an average specific absorption rate (SAR) of 83.5 W/g. Cytotoxicity studies demonstrated that the particles have low cytotoxicity over long exposure times at low concentrations. A fibrinogen clotting assay was used to determine the binding affinity of CREKA-conjugated particles, which was significantly greater than the binding affinity of dextran, only coated iron oxide nanoparticles demonstrating the potential for this particle system to effectively home to a variety of tumor locations. Finally, it was shown that in vitro MMH increased the effects of cisplatin compared to cisplatin or MMH treatments alone.

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“…Dehydrochlorination reaction between two macromolecules containing OH and Cl− substitute, respectively, is realized providing formation of a crosslinking structure 25. In our previous study, we prepared a number of different dextran‐epichlorohydrin hydrogels by varying the amounts of crosslinker in the polymer mixture 29. By taking into account the swelling and mechanical properties of all hydrogels in this study, we decided to use only the gel prepared with 50% (wt) epichlorohydrin.…”

Section: Resultsmentioning

confidence: 99%

Controlled release of EGF and bFGF from dextran hydrogels in vitro and in vivo

2005

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In the present study, dextran-epichlorohydrin hydrogels were employed as carriers for the controlled release of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). The hydrogels were synthesized from 50% (by weight) monomeric cross-linker, epichlorohydrin, containing dextran mixtures by intermolecular side-chain reaction of dextran-hydroxyl groups with epichlorohydrin-epoxy groups. The hydrogel disks of 3-mm diameter and 1.5-mm thickness have a high swelling capacity (EWC = 650%) and enough mechanical stability for the studies in vivo. Impregnation of EGF and bFGF into the dried hydrogels was carried out by use of phosphate buffered saline solution (PBS, pH = 7.4) containing 0.5 microg mL(-1) EGF and 0.1 microg mL(-1) bFGF, respectively. The in vitro release of growth factors was detected by fluorescence spectroscopy. The prolonged release of EGF is continued up to the 14th day, in comparison with a 26-day release of bFGF. The in vivo studies were realized with subcutaneously implanted hydrogels in Wistar albino rats. The rate of neovascularization was analyzed statistically using one-way analysis of significance with EGF and bFGF incorporated hydrogels. In conclusion, dextran-epichlorohydrin hydrogels were shown to be an alternative delivery system for the release of growth factors.

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Synthesis and characterization of dextran hydrogels prepared with chlor‐ and nitrogen‐containing crosslinkers

Cited by 22 publications

References 25 publications

Dextran Nanoparticles Cross‐Linked in Aqueous and Aqueous/Alcoholic Media

Dextran Nanoparticles Cross‐Linked in Aqueous and Aqueous/Alcoholic Media

Synthesis and characterization of CREKA-conjugated iron oxide nanoparticles for hyperthermia applications

Controlled release of EGF and bFGF from dextran hydrogels in vitro and in vivo

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