Synthesis and Characterization Of Dextran Coated Magnetite Nanoparticles Loaded With Curcumin And In Vitro Cytotoxicity Study In MCF-7 Cancer Cells

Abstract

Medicinal plants are considered to be bioactives valuable sources of anti-cancer. But these compounds are hydrophobic in nature and thus show decreased bioavailability. To overcome this issue, biocompatible drug delivery agents could be conjugating with these bioactives to improve their therapeutic efficacy. In the present study Curcumin loaded dextran coated Fe3O4NPs was synthesized by chemical precipitation method and coated with Dextran (DEX) using diffusion method. The structural, morphological and the magnetic properties of the prepared materials were studied by using Fourier transform infrared (FT-IR) spectro-scopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), zeta potential and Vibrating Sample Magnetometer (VSM). The MTT (3-(4,5-dimethylthiahiazol-2-yl)-2,5-diphenyl tetrazolium) assay of CUR loaded dextran coated magnetite nanoparticles (CUR/DEX/Fe3O4)NPs exhibited notable toxicity against MCF7 cells in a dose and time dependant manner. The IC50 values were 177µg/ml and 109µg/ml after 24h and 48h incubation respectively.

A magnetically controlled microfluidic device for concentration dependent in vitro testing of anticancer drug

“…This fold of concentration variation is highly suitable for testing the efficacy of magnetic drugs, as reported in several studies. [52][53][54][55][56][57] In addition, by adjusting the flow rate, it is possible to generate a typical order (1.5-2.2) of concentration ranges for the compartmentalized sample (Fig. S2 †).…”