Synthesis and Characterization of Bio-Active GFP-P4VP Core–Shell Nanoparticles

Sarnello, Erik; Liu, Yuzi; Palen, Bethany; Sun, Elaine; Zuo, Xiaobing; Xu, Tao; Li, Tao

doi:10.3390/catal10060627

Cited by 6 publications

(7 citation statements)

References 53 publications

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“…Based on the described solution volumes, reactions involving reagents of equal concentrations results in a polymer-to-protein mass ratio of 0.24. Previous work has discussed the ability to control the size of polymer-protein CSNPs by altering the polymer solution volume and, therefore, the polymer-protein mass ratio [1,2]. To further investigate the limits of the polymerprotein self-assembly method, BSA and P4VP solutions were prepared at concentrations of 0.5, 1.0, 2.0, 4.0, 6.0, 10.0, and 15.0 mg/mL.…”

Section: Bsa-p4vp Core-shell Nanoparticle Synthesismentioning

confidence: 99%

“…The often dynamic nature of these materials allow for the design of materials with self-healing, cytocompatibility, and biodegradable properties. Recent work has described the preparation of polymer-protein core-shell nanoparticles (CNSPs) [1][2][3][4][5]. These materials are prepared via a stepwise self-assembly process resulting a polymer core covered by a protein "corona" shell.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Advanced Characterization of Polymer–Protein Core–Shell Nanoparticles

Sarnello

2021

Catalysts

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Enzyme immobilization techniques are widely researched due to their wide range of applications. Polymer–protein core–shell nanoparticles (CSNPs) have emerged as a promising technique for enzyme/protein immobilization via a self-assembly process. Based on the desired application, different sizes and distribution of the polymer–protein CSNPs may be required. This work systematically studies the assembly process of poly(4-vinyl pyridine) and bovine serum albumin CSNPs. Average particle size was controlled by varying the concentrations of each reagent. Particle size and size distributions were monitored by dynamic light scattering, ultra-small-angle X-ray scattering, small-angle X-ray scattering and transmission electron microscopy. Results showed a wide range of CSNPs could be assembled ranging from an average radius as small as 52.3 nm, to particles above 1 µm by adjusting reagent concentrations. In situ X-ray scattering techniques monitored particle assembly as a function of time showing the initial particle growth followed by a decrease in particle size as they reach equilibrium. The results outline a general strategy that can be applied to other CSNP systems to better control particle size and distribution for various applications.

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Section: Bsa-p4vp Core-shell Nanoparticle Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and Advanced Characterization of Polymer–Protein Core–Shell Nanoparticles

Sarnello

2021

Catalysts

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“…The accepted range for particle sizes for biomedical applications is around 100 nm [70] . Regardless of the application, the use of core‐shell particles allows the use of enzymes efficiently in different environments [71] …”

Section: Core‐shell Polymeric Particlesmentioning

confidence: 99%

“…[70] Regardless of the application, the use of core-shell particles allows the use of enzymes efficiently in different environments. [71] Concerning the application of core-shell particles as a support for making heterogeneous biocatalysts, the possibilities are as vast as those that employ other inorganic and polymeric structures as enzymatic support. For example, the use of lipase for biodiesel production is widely discussed in the literature, generally showing good results both in free and immobilized form.…”

Section: Applicationsmentioning

confidence: 99%

A Comprehensive Review on Core‐Shell Polymeric Particles for Enzyme Immobilization

et al. 2022

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The search for ideal solid support that promotes enzyme stability, easy separation and reuse cycles in different processes has grown widely. In this sense, core‐shell polymer particles draw attention because they are already widely used in adsorption, catalysis, and drug delivery, due to their remarkable advantages in surface properties adjustment, excellent mechanical stability, and high chemical resistance, as a result of the combination of the characteristics of the polymers that form the core and shell of the structure. Thus, this review article provides an overview of the synthesis processes of these particles, highlighting the characteristics that can be obtained from each synthesis technique, as well as the most recently used techniques to obtain polymeric particles with core‐shell morphology. The advantages and main challenges for the application of these structures in the immobilization of enzymes are also discussed, providing a general summary of what has been explored in the literature. Despite the potential of application, the use of these materials in the immobilization of enzymes is still little explored, with studies focused mainly on lipases and around the same classes of polymers. Therefore, there is an opportunity window regarding the investigation of the immobilization of other enzymes of commercial interest on these polymeric supports.

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“…The polymer consists of multiple 4-vinylpyridine monomers attached at the C 4 positions of each monomer. Poly(4-vinylpyridine) (P4VP) is an effective candidate due to the nitrogen atom in its pyridine group being a strong hydrogen-bond acceptor [51][52][53]. Suthiwangcharoen and Li et al generated novel Core-Shell nanoparticles by P4VP to immobilize glucose oxidase and horseradish peroxidase on the single particles, the activity of enzymes was increased about 20% compared with free enzymes [54].…”

Section: Synthetic Polymersmentioning

confidence: 99%

Immobilization of Enzymes by Polymeric Materials

et al. 2021

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Enzymes are the highly efficient biocatalyst in modern biotechnological industries. Due to the fragile property exposed to the external stimulus, the application of enzymes is highly limited. The immobilized enzyme by polymer has become a research hotspot to empower enzymes with more extraordinary properties and broader usage. Compared with free enzyme, polymer immobilized enzymes improve thermal and operational stability in harsh environments, such as extreme pH, temperature and concentration. Furthermore, good reusability is also highly expected. The first part of this study reviews the three primary immobilization methods: physical adsorption, covalent binding and entrapment, with their advantages and drawbacks. The second part of this paper includes some polymer applications and their derivatives in the immobilization of enzymes.

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Synthesis and Characterization of Bio-Active GFP-P4VP Core–Shell Nanoparticles

Cited by 6 publications

References 53 publications

Synthesis and Advanced Characterization of Polymer–Protein Core–Shell Nanoparticles

Synthesis and Advanced Characterization of Polymer–Protein Core–Shell Nanoparticles

A Comprehensive Review on Core‐Shell Polymeric Particles for Enzyme Immobilization

Immobilization of Enzymes by Polymeric Materials

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