Synthesis and Characterization of a Fe3O4@PNIPAM-Chitosan Nanocomposite and Its Potential Application in Vincristine Delivery

Abstract

In this research, we conducted a systematic evaluation of the synthesis parameters of a multi-responsive core-shell nanocomposite (Fe3O4 nanoparticles coated by poly(N-isopropylacrylamide) (PNIPAM) in the presence of chitosan (CS) (Fe3O4@PNIPAM-CS). Scanning electron microscopy (SEM) was used to follow the size and morphology of the nanocomposite. The functionalization and the coating of Fe3O4 nanoparticles (Nps) were evaluated by the ζ-potential evolution and Fourier Transform infrared spectroscopy (FTIR). The nanocomposite exhibited a collapsed structure when the temperature was driven above the lower critical solution temperature (LCST), determined by dynamic light scattering (DLS). The LCST was successfully shifted from 33 to 39 °C, which opens the possibility of using it in physiological systems. A magnetometry test was performed to confirm the superparamagnetic behavior at room temperature. The obtained systems allow the possibility to control specific properties, such as particle size and morphology. Finally, we performed vincristine sulfate loading and release tests. Mathematical analysis reveals a two-stage structural-relaxation release model beyond the LCST. In contrast, a temperature of 25 °C promotes the diffusional release model. As a result, a more in-depth comprehension of the release kinetics was achieved. The synthesis and study of a magnetic core-shell nanoplatform offer a smart material as an alternative targeted release therapy due to its thermomagnetic properties.

AS1411 conjugated magnetic‐based poly N‐isopropyl acrylamide nanoparticles for delivery of erlotinib to prostate cancer cells

“…The functional groups at 1030 (COC, PNIPAM), 2855, and 2922 (CH 2 ) cm −1 demonstrate the formation of SPION‐PNIPAM NPs. [ 28,29 ] The conjugation of the SPIONs to PNIPAM‐NHS was confirmed at 1500–1740 (COO) cm −1 . The main peaks for ERL are 3256 (CH), 1632 (NH), and 1075 (CO, phenyl ether).…”

Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction

“…A band is observed from 1308 cm −1 to 1232 cm −1 due to hydrogen bonds [ 27 ]. Finally, a signal is observed at 1068 cm −1 , indicating the presence of C-O-C stretching of ester groups [ 31 ].…”

“…Samples were diluted 1 to 10 before deposition on an aluminum film. FTIR spectra were collected on a Spectrum spectrometer (Perkin Elmer Inc., Waltham, MA, USA) equipped with a single attenuated total reflectance (ATR) diamond set to a range of 400 cm −1 to 4000 cm −1 [ 31 ].…”

Poly(N-isopropylacrylamide)-Based Hydrogels for Biomedical Applications: A Review of the State-of-the-Art

“…Accordingly, systems such as, PNIPAM@ magnetic NPs (Fe 3 O 4 )/5-fluorouracil (5FU) and oxaliplatin (OXA)- acrylic acid (AA), 3-butenoic acid (3BA) or allylamine (AL) (pNIPAM@ Fe 3 O 4 /5FU/OXA-AA, 3BA, AL) [ 181 ], doxorubicin(DOX)-gold nanorods (GNRs)-PNIPAM@poly (d, l-lactide)-poly (ethylene glycol) (PLA-PEG) (DAPP)micelles (DOX-GNRs-PNIPAM@PEG-PLA,DAPP) [ 182 ], vancomycin (VANCO)/SF-Na Alg NPs/PNIPAM@epidermal growth factor (EGF) (VANCO/SF-Na Alg NPs/PNIPAM@EGF) [ 183 ], PNIPAM- superparamagnetic iron oxide nanoparticles (SPION)- etoposide (SPION-PNIPAM-Etoposide) [ 184 ], NaALG-g-P(NIPAM 80 - co -NtBAM 20 )/Dulbecco’s modified Eagle’s medium (DMEM) (NaALG-g-P(NIPAM 80 - co -NtBAM 20 )/DMEM) [ 185 ] and many more have been designed by researchers in order to intensify the use of PNIPAM polymer in the field of drug delivery [ 186 , 187 ]. Table 1 depicts the latest investigations of PNIPAM-based hydrogels in drug delivery.…”