Synthesis and Characterization of a Novel Heterocyclic Schiff Base and Development of a Fluorescent Sensor for Vitamin B12

Synthesis, characterization, DFT studies and evaluation of the potential anti-tumour activity of nicotinic hydrazide based Schiff base using in vitro and molecular docking techniques

“…High intensity bands in this spectrum around 350–360 nm were attributed to the n- π*transitions of C N [ 36 ]. While performing experimental calculation of BNH, the intensity peak is observed at 357 nm point out the presence of the n- π* transition of C N azomethine group [ 37 ]. Theoretical calculation is done using DFT study which shows a broad spectrum with an intensity peak of 351 nm Fig.…”

Bidirectional ATP-driven transport of cobalamin by the mycobacterial ABC transporter BacA

“…Hence, development of alternative transport assays that support high-throughput experiments are desirable. Methods established in the past to detect cobalamin in solution or in cellular or liposomal compartments without the use of radioactive substrates [32][33][34][35][36][37][38][39][40][41] often do not allow for high-throughput measurements, or are incompatible with assays to measure transport in both import and export direction. We chose to use a protein-based sensor derived from the SBP BtuF 25 .…”