Synthesis and characterization of a penicillin-poly(L-lysine) which is recognized by human IgE anti-penicillin antibodies

Abstract

Conjugates of poly(L-lysine) containing a penicillin or a penicilloyl residue were prepared and characterized by 1H NMR and by size-exclusion HPLC. These conjugates and conjugates with human serum albumin were used in radioallergosorbent tests (RAST) for the determination of allergy toward penicillins.

Synthesis and Synergistic Antimicrobial Efficacy of Covalent Conjugates Composed of Epsilon-Poly-l-lysine and Beta-Lactam Antibiotics

“…The results are presented in Table , which illustrates a reduction in the load ratios of penicillin G against EPL in an acidic environment. Additionally, it is important to note that the β-lactam ring is susceptible to cleavage in both highly acidic and basic aqueous solutions, and the environmental pH can potentially influence the process of active ester synthesis. , To explore this effect, experiments were conducted to investigate the influence of solution pH on the stability of the β-lactam ring in aqueous environments, ranging from pH 5 to 7. The area ratio between the hydrogen signals at 5.25–5.5 ppm (β-lactam ring) and 7.0–7.5 ppm (benzyl hydrogen) served as an indicator of the β-lactam ring’s integrity and covalent conjugate stability during synthesis (refer to Figure S4).…”