Synthesis and Characterization of a Cascaded Chromogenic Enzyme Substrate

Abstract

A novel galactoside derivative (1) incorporating p-nitroaniline (p-NA) as chromophore has been synthesized and used as a cascaded chromogenic enzyme substrate for the detection of β-D-galactosidase, which was characterized using UV, 1H NMR, 13C NMR and MS spectra. A plausible mechanism for the release of p-NPA from the enzyme substrate 1 was proposed.