Synthesis and characterization of 68Ga labeled Fe3O4 nanoparticles for positron emission tomography (PET) and magnetic resonance imaging (MRI)

In VitroPET/MRI Diagnosis and Targeted Chemotherapy for Cancer Using Radiolabeled Nanoprobe : A Theragnostic Approach

“…C. The cell viability was calculated by recording absorbance at 450 nm. 8,10 %Viability = absorbance of sample=absorbance of control ð Þ ×100 resultant figure, the samples FHFCNP and NFHFCNP show absorption peaks at 280 and 360 nm confirming the covalent attachment of the folate with NPs. 15 But the corresponding folate absorption peaks were not observed in HFCNP and NHFCNP samples.…”

“…19-0629, a = 8.396 Å) have a negative surface charge because of the presence of carboxylate group and the average particle size was~9 nm ( Figure S2). 8 From the prepared FCNP (4 mg/mL) solution, 5 mL were stirred with 100 μL of iodomethane at 40 C for 12 h and washed with methanol (12 000 rpm, 10 min). Then, 100 μL of hydrazine were added to the mixture and refluxed at 40 C for 5 h and stirred overnight at room temperature (RT).…”

“…Finally, the resulting mixture (NFHFCNP) was washed and dispersed in ethanol solution. 8 The structure of synthesized HFCNP and NFHFCP are shown in Figure 1. The NFHFCNP has folate and NOTA as a chelate agent on the surface and the HFCNP has hydrazide with citrate group on the surface.…”

“…After 10 min, the mixture was thoroughly washed and dispersed in deionized water (DW GaNFHFCNP were measured using ITLC-SG eluted with 0.1 M citric acid as a mobile phase. 8,26 The AR-2000 radio-TLC Imaging Scanner and CRC dosemeter were used to measure the radioactivity. In Vitro Stability of 68 Ga Labeling of HFCNP and NFHFCNP in Human Serum.…”

“…One of the alternative approach is to use metal radionuclide including 68 Ga (t 1/2 = 68 min, β + = 89% and electron capture (EC) = 11%), which can be obtained easily from 68 Ge/ 68 Ga generator without the serve of cyclotron. 8 [20][21][22] .…”