Synthesis and characterisation of Thio-H, a new excitation and emission ratioable fluorescent Ca2+/Mg2+ indicator with high brightness

Cielen, Els; Stobiecka, Agnieszka; Tahri, Abdellah; Hoornaert, Georges J.; Schryver, F. C. De; Gallay, Jacques; Vincent, Michel; Boens, Noël

doi:10.1039/b107042h

Cited by 46 publications

(32 citation statements)

References 35 publications

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“…Nonlinear curve fitting of Eq. (1) [17] to the fluorescence data F recorded as a function of [Zn 2+ ] yields values of K d , the fluorescence signals F min and F max at minimal and maximal [Zn 2+ ], respectively (corresponding to the free and Zn 2+ bound forms of the probe, respectively), and n (the number of Zn 2+ bound per probe). The ground-state dissociation constant K d was found to be 24.0 ± 4.3 μM.…”

mentioning

confidence: 99%

A sensitive Schiff-base fluorescent indicator for the detection of Zn2+

Wang

Qin

Liu

2010

Inorganic Chemistry Communications

116

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confidence: 99%

A sensitive Schiff-base fluorescent indicator for the detection of Zn2+

Wang

Qin

Liu

2010

Inorganic Chemistry Communications

116

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“…Determination of K a from Direct Fluorimetric Titration. If the photophysical system depicted in Scheme 1 is excited with light of constant intensity, and if the absorbance A of the sample is less than 0.1, and moreover, if the rate of proton binding in the excited state can be neglected, the total fluorescence signal F(λ ex ,λ em , [H + ]) at proton concentration [H + ], due to excitation at λ ex and observed at emission wavelength λ em can be expressed by 21,22 where F min stands for the fluorescence signal of the basic form of the pH indicator (2*), and F max denotes the fluorescence signal of the acidic form of the pH indicator (1* Fitting nonlinear eq 17 to the excitation ratiometric fluorescence data R as a function of [H + ] yields values for K a , n, R min , and R max . Because (λ em ,λ ex 2 )sthe ratio of the fluorescence signal of phenolate form (2) of BCECF over that of the phenol form (1) at the indicated excitation and emission wavelengthss is experimentally accessible, a value for K a can be recovered from ratiometric excitation fluorescence data.…”

Section: Determination Of K a And Molar Absorption Coefficients From mentioning

confidence: 99%

Photophysics of the Fluorescent pH Indicator BCECF

Boens

Qin²,

Basarić³

et al. 2006

J. Phys. Chem. A

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The photophysical behavior of BCECF [2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein]scurrently the most widely used fluorescent pH indicator for near-neutral intracellular pH measurementsshas been explored by using absorption and steady-state and time-resolved fluorescence measurements. The influence of ionic strength as well as total buffer concentration on the absorbance and steady-state fluorescence has been investigated. The apparent acidity constant of the pH indicator determined by absorbance and fluorescence titration is dependent on the added buffer and salt concentrations. A semiempirical model is proposed to rationalize the variations in the apparent pK a values. The excited-state proton exchange of BCECF at physiological pH becomes reversible upon addition of phosphate buffer, inducing a pH-dependent change of the fluorescence decay times. Fluorescence decay traces collected as a function of total buffer concentration and pH were analyzed by global compartmental analysis yielding the following values of the rate constants describing excited-state dynamics of BCECF: k 01 ) 3.4 × 10 8 s -1 , k 02 ) 2.6 × 10 8 s -1 , k 21 ≈ 1 × 10 6 M -1 s -1 , k 12 B ) 1.4 × 10 8 M -1 s -1 , and k 21 B ) 4.3 × 10 7 M -1 s -1 .

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“…Nonlinear fitting of Eq. (1) [7] to the ground-state fluorescence data F recorded as a function of [Fe 3+ ] yields values of K d to be 0.57 ± 0.07 mM. F max and F min represent fluorescence signals at maximal and minimal [Fe 3+ ], respectively.…”

Section: O M / L O C a T E / I N O C H Ementioning

confidence: 99%