Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

Abstract

Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits...

Probing the mutation independent interaction of DNA probes with SARS-CoV-2 variants through a combination of surface-enhanced Raman scattering and machine learning

“…We propose to select four ASOs, two for the front (ASO1 and ASO2), and two targeting the end region (ASO3 and ASO4) of the gene. The ASOs will also target closely following sequences at each location ( Alafeef et al, 2020 , 2021a , 2021b , 2020 ; Dighe et al, 2021 ; Moitra et al, 2020 , 2021a , 2021b ). Accordingly, four ASOs were selected both for N and E genes, whereas only two were selected for the RdRp gene as ASOs with high enough binding energies are not available for this target ( Table 1 ).…”

“…With increase in SARS-CoV-2 RNA concentration, there will be increase in agglomeration among N-ASO 1+2 AuNPs leading to change in surface plasmon resonance of the gold nanoparticles ( Alafeef et al, 2020 , 2021a , 2021b , 2020 ; Dighe et al, 2021 ; Moitra et al, 2020 , 2021b ). Further, when the AuNP-RNA conjugate of different concentrations was added onto the surface, the molecule will take certain orientation at the nanostructure surface.…”

Monitoring the Viral Transmission of SARS-CoV-2 in Still Waterbodies Using a Lanthanide-Doped Carbon Nanoparticle-Based Sensor Array

“… 78 − 80 Fluorescence emission was therefore selected as an output signal as it provided higher sensitivity than absorption. 74 , 81 , 82 To further investigate the surface chemistry of the synthesized particles, FTIR spectroscopy measurements were carried out. The FTIR spectra ( Figure 2 g) show that the metal-doped CNPs possessed abundant hydrophilic groups on their surface such as O–H and N–H (3260 cm –1 ), which confirmed their dispersibility and stability in aqueous media.…”

Prospects of NIR fluorescent nanosensors for green detection of SARS-CoV-2

“…For instance, oleic acid capped PbS QD requires phase transfer before biomedical applications. [72] In addition, to modify the bioreceptor (ASO) on PbS QD for imaging of SARS-CoV-2 infected samples, UV-induced thiol-ene click chemistry was used, which also required an organic chemical pollutant (dimethoxypropiophenone (DMPA)). Similar to most tests, RNA extraction was a necessary procedure when the sensitivity could not meet the extraction-free analysis.…”