Synthesis and characterisation of multifunctional alginate microspheres via thein situformation of ZnO quantum dots and the graft of 4-(1-pyrenyl) butyric acid to sodium alginate

Abstract

Growth factor-loaded fluorescent alginate microspheres, which can realise sustained growth factor release and fluorescence imaging, were synthesised by in situ formation of ZnO quantum dots (QDs) and covalent graft of 4-(1-pyrenyl) butyric acid (PBA). BSA was chosen as a growth factor model protein to study the release kinetic of growth factors from alginate microspheres. The microsphere size and fluorescent properties were also investigated. Investigations of cell culture were used for evaluating biocompatibility of BSA-loaded fluorescent microspheres and fluorescence imaging property of ZnO QDs and PBA-grafted sodium alginate from the microspheres. The results show that they have good fluorescent property either to microspheres or to cells and fluorescent microspheres have good biocompatibility and property in sustained release of growth factors. The obtained microspheres will be expected to realise the imaging of cells and materials and also the release of growth factor in tissue engineering or in cell culture.

Hybrid use of combined and sequential delivery of growth factors and ultrasound stimulation in porous multilayer composite scaffolds to promote both vascularization and bone formation in bone tissue engineering

“…The related degradation and in vitro culture results of the precursors, which were used for multilayer composite scaffolds, had been reported in our previous studies …”

“…The related degradation and in vitro culture results of the precursors, which were used for multilayer composite scaffolds, had been reported in our previous studies. 4,6,26,27 Animal experiments The animal groups were divided into four groups where different scaffolds were used. Group I was the control group, in which the used scaffolds were porous calcium phosphate ceramic ones without coating anything.…”

13-93 bioactive glass/alginate composite scaffolds 3D printed under mild conditions for bone regeneration

“…4,5,17 Based on this background, biodegradable organic-inorganic composite scaffolds for bone regeneration were fabricated in this present study using 3D printing under mild conditions. Biocompatible and biodegradable alginate has been widely used in tissue engineering, growth factor and cell delivery, 12,[20][21][22][23] and was selected as the organic component in this study. It is also a suitable biomaterial for preparing functional hydrogels, due to its water-solubility and cross-linking with ionic response.…”

Chondro‐Inductive b‐TPUe‐Based Functionalized Scaffolds for Application in Cartilage Tissue Engineering

“…[16] No use of PBA over PU surfaces has been previously published, and the only use of PBA with application in TE was done by Luo et al in 2015. [44] Its PBA consists of a pyrene group that contains 𝜋 electrons and a carboxylic group that can be used to facilitate further functionalization. In the present study, b-TPUe has not had a high density of delocalized 𝜋-electrons like graphene so it is not possibly established a 𝜋-𝜋 stacking between b-TPUe and PBA.…”

“…in 2015. [ 44 ] Its PBA consists of a pyrene group that contains π electrons and a carboxylic group that can be used to facilitate further functionalization. In the present study, b‐TPUe has not had a high density of delocalized π ‐electrons like graphene so it is not possibly established a π ‐ π stacking between b‐TPUe and PBA.…”