Synthesis and characterisation of a decapeptide having LH-RH/FSH-RH activity

Geiger, Rolf; König, Wolfgang; Wissmann, Hans; Geisen, K.; Enzmann, F.

doi:10.1016/0006-291x(71)90483-9

Cited by 104 publications

(21 citation statements)

References 6 publications

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“…The availability of synthetic LH-releasing hormone/FSHreleasing hormone (14)(15)(16) opens new possibilities for study of the mechanism of action of this neurohormone in the adenohypophysis. Confirming previous observations made both iF vivo and in vitro (4-8, 10, 13, 42-45) …”

Section: Discussionmentioning

confidence: 99%

“…Since this peptide stimulates the release of both LH and FSH under various experimental conditions (4-8, 10-13), it has been called the LH-releasing hormone/FSH-releasing hormone (4,5,7) or the gonadotropin-releasing factor (12). The availability of synthetic LH-releasing hormone/FSHreleasing hormone (14)(15)(16) and of its analogs is of obvious importance for studies of the mechanism of action of this neurohormone in the adenohypophysis.…”

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confidence: 99%

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Stimulation of Adenosine 3′:5′-Cyclic Monophosphate Accumulation in Anterior Pituitary Gland In Vitro by Synthetic Luteinizing Hormone-Releasing Hormone

Borgeat

Chavancy

Dupont

et al. 1972

Proc. Natl. Acad. Sci. U.S.A.

183

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A near-maximal dose (20 ng/ml) of synthetic luteinizing hormone(LH)-releasing hormone/follicle-stimulating hormone(FSH)-releasing hormone added to incubated anterior pituitary tissue of male rats leads to concomitant increases of intracellular concentrations of adenosine 3': 5'-monophosphate and of release of both LH and FSH. The stimulatory effect of LH-releasing hormone/ FSH-releasing hormone is observed after a lag period of about 90 min and is progressive at later time intervals; a 3-fold stimulation of cAMP accumulation over control is seen after 210 min of incubation. Half-maximal stimulation of cAMP accumulation is observed between 0.1 and 1.0 ng/ml (0.1-1 nM) of LH-releasing hormone/FSH-releasing hormone. In the presence of 10 mM theophylline, the stimulatory effect of LH-releasing hormone/FSH-releasing hormone on cAMP accumulation is similar to that observed in the absence of the inhibitor of cyclic nucleotide phosphodiesterase, indicating that the releasing hormone exerts its effect by specific activation of adenylate cyclase in LH-and FSH-secreting cells rather than by inhibition of cyclic nucleotide phosphodiesterase. Since the release of growth hormone, thyrotropin, prolactin, and adrenocorticotropic hormone is not affected by LH-releasing hormone/FSH-releasing hormone, and since cAMP stimulates the release of all six adenohypophyseal hormones. the observed changes of cAMP concentrations indicate specific stimulation of adenylate cyclase activity in LHand FSH-secreting cells of the adenohypophysis.Secretion of luteinizing hormone (LH) and of follicle-stimulating hormone (FSH) by the anterior pituitary gland is controlled by a neurohormone released from the hypothalamic area and carried to its specific adenohypophyseal site of action by a portal blood system (1-4). Recently, after more than 10 years of research in many laboratories, this neurohormone was isolated from porcine (5-7) and ovine (8) hypothalami and characterized as a decapeptide having the following structure: (pyro)Glu-His-Try-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 (9-11). Since this peptide stimulates the release of both LH and FSH under various experimental conditions (4-8, 10-13), it has been called the LH-releasing hormone/FSH-releasing hormone (4, 5, 7) or the gonadotropin-releasing factor (12). The availability of synthetic LH-releasing hormone/FSHreleasing hormone (14-16) and of its analogs is of obvious importance for studies of the mechanism of action of this neurohormone in the adenohypophysis.There was suggestive evidence for a role of adenosine 3': 5'-cyclic monophosphate (cAMP) as a mediator of the action of LH-releasing hormone/FSH-releasing hormone. These studies pertained to reported stimulatory effects of N6,2'.-Odibutyryl cAMP and theophylline (17) (20, 21). However, definitive proof of the role of the adenylate cyclase system as mediator of the action of LH-releasing hormone/FSH-releasing hormone could be obtained only by measurements of adenohypophyseal adenylate cyclase activity or cAMP concentrations under the inf...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Stimulation of Adenosine 3′:5′-Cyclic Monophosphate Accumulation in Anterior Pituitary Gland In Vitro by Synthetic Luteinizing Hormone-Releasing Hormone

Borgeat

Chavancy

Dupont

et al. 1972

Proc. Natl. Acad. Sci. U.S.A.

183

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“…The synthetic LH-RH (decapeptide) used in all experiments was obtained from Hoechst Pharmaceuticals Ltd and was synthesized by the method of Geiger, König, Wissman, Geisen & Enzmann (1971).…”

Section: Methodsmentioning

confidence: 99%

The Gonadotrophic Hormone Response of Anoestrous and Cyclic Ewes to Synthetic Luteinizing Hormone-Releasing Hormone

Symons¹,

Cunningham²,

Saba³

1974

Reproduction

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“…The determination for LH-releasing hormone (LH-RH) of a decapeptide structure (Matsuo, Baba, Nair, Arimura & Schally, 1971) and synthesis of this material (Geiger, König, Wissmann, Geisen & Enzmann, 1971) has provided a valuable tool for the study of reproductive function in the sheep.…”

Section: Introductionmentioning

confidence: 99%

Progesterone Levels Following Treatment of Seasonally Anoestrous Ewes With Synthetic Lh-Releasing Hormone

Haresign¹,

Foster²,

Haynes³

et al. 1975

Reproduction

114

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Summary. Plasma progesterone determinations were carried out on blood samples collected daily from Clun Forest ewes during the normal oestrous cycle and also after administration of LH-releasing hormone (LH-RH) during seasonal anoestrus.Levels of plasma progesterone at oestrus ranged from 0\m=.\1to 0\m=.\5 ng/ml and luteal phase levels from 3 to 6 ng/ml. Levels found during seasonal anoestrus were within the range of those observed at oestrus. Following treatment with LH-RH, there was an increase in the plasma LH level in all cases and ovulation occurred in twenty-three out of twenty-seven treated ewes. In the animals which ovulated, the plasma progesterone concentration either remained basal (eighteen animals) or rose to a lower level (<2 ng/ml) than that found during the luteal phase of the cycle.

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Synthesis and characterisation of a decapeptide having LH-RH/FSH-RH activity

Cited by 104 publications

References 6 publications

Stimulation of Adenosine 3′:5′-Cyclic Monophosphate Accumulation in Anterior Pituitary Gland In Vitro by Synthetic Luteinizing Hormone-Releasing Hormone

Stimulation of Adenosine 3′:5′-Cyclic Monophosphate Accumulation in Anterior Pituitary Gland In Vitro by Synthetic Luteinizing Hormone-Releasing Hormone

The Gonadotrophic Hormone Response of Anoestrous and Cyclic Ewes to Synthetic Luteinizing Hormone-Releasing Hormone

Progesterone Levels Following Treatment of Seasonally Anoestrous Ewes With Synthetic Lh-Releasing Hormone

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