Synthesis and cellular biocompatibility of two kinds of HAP with different nanocrystal morphology

Abstract

Two kinds of hydroxyapatite (HA) with different nanocrystal morphology were obtained via a simple aqueous precipitation method under different reactants molar ratios. Under Ca/P molar ratio of 1.67/1, rod-like crystal was produced, while under Ca/P molar ratio of 1.80/1, spherical crystal was produced. The spherical crystal was 40-60 nm in diameter, while the rod-like crystal was 40-55 nm in diameter and 79-100 nm in length. The influence of HA nanocrystal morphology on osteoblasts growth was assayed by MTT method and SEM. The results indicated that there was a significantly higher absorbency value on the surface of HA with spherical crystal in MTT assay than the latter. In the process of SEM observation, it is found that osteoblasts spread out a large quantity of nano-filopodias on spherical crystal surface, thus exhibiting much more active cell morphology. In conclusion, HA with spherical nanocrystal showed more favorable properties than that with rod-like one for osteoblasts.

In vitro studies of osteoblasts response onto zinc aluminate ceramic films

“…The semi-spherical nanophase as showed by the images of the topography by AFM has a grain size of around 30-100 nm and it is clear that the cell functionality and biocompatibility of the osteoblasts cells to ZnAl 2 O 4 films will be influenced by the semi-spheroid mor- phology of the surface features. Similar results have been reported for enhance adhesion and biological response to nanostructure materials and had indicated that grain size plays a crucial role in mediating on the cell adhesion to nanophase ceramics and also had been reported that spheroids material surface should be more beneficial to proliferation of osteoblastic cells [32][33][34][35][36] . Finally, Figure 5 shows the morphology of osteoblasts cells cultured onto ZnAl 2 O 4 films.…”

Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cells

“…In our study, two kinds of HAP (rod-like and sphere-like) were obtained. The cell experiment showed that HAP with spherical nanocrystals showed more favorable properties than rod-like HAP for osteoblasts, which was also demonstrated by Zhao et al [32]. The explanation for the favorable effect of sphere-like nano-HAP on osteoblasts might be the well-organized surface which seems beneficial for filopodia protrusion.…”

Nanohydroxyapatite shape and its potential role in bone formation: an analytical study

“…A previous study compared ALP activity following NP exposure to two rod-shaped NPs of different sizes and did not find any significant difference between them [19,21]. Another study looking at an osteosarcoma-derived cell line found that cell proliferation was decreased in the presence of spherical-shaped HA NPs [21]. The group also reported a change in cell morphology, as observed via SEM, although this was not based on ultrastructural analysis of ECM proteins or intracellular ion concentration.…”

“…Perkin Elmer Imaging Suite version 4.1 was used to collect the data, between the range of 6000 -250 cm 21 , and the background signal was subtracted from sample data.…”

“…Another study found that rod-shaped particles caused primary osteoblasts to decrease their proliferation after 3 days of culture compared with spherical-shaped. A change of morphology was also visible under scanning electron microscopy (SEM) [21]. The effect of different-shaped NPs on the intracellular ion content of primary osteoblasts, as measured using TEM-based microanalysis, as well as ultrastructural and ECM changes other than NP internalization [22] have not, to the authors' knowledge, been previously investigated [18].…”