Homonoia riparia Lour (H. riparia) leaf extract was used in the current study to reduce silver nitrate's chemical reduction, resulting in green synthesis of silver nanoparticles to test the antibacterial activity and cytotoxic effects. The plant H. riparia was chosen due to the presence of reducing agents and the need for more information on the synthesis of silver nanoparticles using said plant. Environmentally friendly method was developed in this study for synthesising AgNPs from Homonoia riparia Lour (H. riparia) leaf extract. UV-vis absorption, XRD, and EDX analyses verified the synthesis of AgNPs. Analysis by SEM and TEM revealed that AgNPs were roughly spherical in shape and 27 nm in size. Maximum absorbance was measured between 440 and 450 nm, the zeta potential was -23.4 mV, and the powder crystal structures were cubic. The absorbance of AgNPs peaked between 400 and 430 nm. The MICs (mg/ml) of the synthesised AgNPs on Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 10536, Pseudomonas aeruginosa ATCC 15442, and Klebsiella pneumoniae producing carbapenemase ATCC BAA-2146 were tested. After verifying the preliminary analysis results, which showed that all of the tested samples have cytotoxic potential, their cytotoxic activity was again evaluated, this time against the same strains, to determine the IC50. Values were expressed as a percentage of cell growth inhibition at 50 against pathogenic microorganisms when used at lower doses and exhibit a more potent antibacterial effect.