Synthesis and biological activities of a phosphorodithioate analog of 2′, 5′-oligoadenylate

Beigelman, Leo; Matulić‐Adamić, Jasenka; Haeberli, Peter; Usman, Nassim; Dong, Bin; Silverman, Robert H.; Khamnei, Shahrzad; Torrence, Paul F.

doi:10.1093/nar/23.19.3989

Cited by 29 publications

(13 citation statements)

References 29 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the interesting approaches is to invert nucleotide on the 3' side of aptamer. By obtaining two 5' ends, an aptamer becomes resistant to 3'-exonucleases activity, which dominantly occurs in the extracellular environment [ 117 ]. Another modifications leading to enhance the stability of aptamers are various changes in the ribose 2' site [ 4 , 6 , 11 ].…”

Section: Aptamers—“for” and “Against”mentioning

confidence: 99%

Aptamers in Diagnostics and Treatment of Viral Infections

Wandtke

Woźniak

Kopiński

2015

Viruses

112

109

View full text Add to dashboard Cite

Aptamers are in vitro selected DNA or RNA molecules that are capable of binding a wide range of nucleic and non-nucleic acid molecules with high affinity and specificity. They have been conducted through the process known as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). It serves to reach specificity and considerable affinity to target molecules, including those of viral origin, both proteins and nucleic acids. Properties of aptamers allow detecting virus infected cells or viruses themselves and make them competitive to monoclonal antibodies. Specific aptamers can be used to interfere in each stage of the viral replication cycle and also inhibit its penetration into cells. Many current studies have reported possible application of aptamers as a treatment or diagnostic tool in viral infections, e.g., HIV (Human Immunodeficiency Virus), HBV (Hepatitis B Virus), HCV (Hepatitis C Virus), SARS (Severe Acute Respiratory Syndrome), H5N1 avian influenza and recently spread Ebola. This review presents current developments of using aptamers in the diagnostics and treatment of viral diseases.

show abstract

Section: Aptamers—“for” and “Against”mentioning

confidence: 99%

Aptamers in Diagnostics and Treatment of Viral Infections

Wandtke

Woźniak

Kopiński

2015

Viruses

112

109

View full text Add to dashboard Cite

show abstract

“…As other polynucleotides, siRNA also suffers from poor stability in biological fluids and restricted capacity to enter cells. Different chemical modifications have been performed in the structure of polynucleotides especially to ameliorate their stability when in contact with biologic fluids thus, improving their bioavailability (Beigelman et al, 1995;Beverly et al, 2006;Chiu and Rana, 2003;Epstein and Kurz, 2007;Hall et al, 2004).…”

Section: Small Interfering Rna (Sirna)mentioning

confidence: 99%

Synthetic nanocarriers for the delivery of polynucleotides to the eye

Saraiva

Castro-López

Pañeda³

et al. 2017

European Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

This review is a comprehensive analysis of the progress made so far on the delivery of polynucleotide-based therapeutics to the eye, using synthetic nanocarriers. Attention has been addressed to the capacity of different nanocarriers for the specific delivery of polynucleotides to both, the anterior and posterior segments of the eye, with emphasis on their ability to (i) improve the transport of polynucleotides across the different eye barriers; (ii) promote their intracellular penetration into the target cells; (iii) protect them against degradation and, (iv) deliver them in a long-term fashion way. Overall, the conclusion is that despite the advantages that nanotechnology may offer to the area of ocular polynucleotide-based therapies (especially AS-ODN and siRNA delivery), the knowledge disclosed so far is still limited. This fact underlines the necessity of more fundamental and product-oriented research for making the way of the said nanotherapies towards clinical translation.

show abstract

“…However, these nucleic acid‐based aptamers are susceptible to nucleolytic degradation in the biological matrix. To increase their stability and circulating time in the body, several aptamer modifications (either in the base or the backbone), such as with phosphorothioate or methylphosphonate, have been developed . In addition to the modification of functional groups on the ends of aptamers for enhancement of stability, conjugation of aptamers onto the surface of AuNPs increases the resistance in a biological matrix .…”

Section: Conjugation Of Aunps With Aptamersmentioning

confidence: 99%

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

Wang

et al. 2016

ChemBioChem

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) are useful for diagnostic and biomedical applications, mainly because of their ease in preparation and conjugation, biocompatibility, and size-dependent optical properties. However, bare AuNPs do not possess specificity for targets. AuNPs conjugated with DNA aptamers offer specificity for various analytes, such as proteins and small molecules/ions. Although DNA aptamers themselves have therapeutic and target-recognizing properties, they are susceptible to degradation in vivo. When DNA aptamers are conjugated to AuNPs, their stability and cell uptake efficiency both increase, making aptamer-AuNPs suitable for biomedical applications. Additionally, drugs can be efficiently conjugated with DNA aptamer-AuNPs to further enhance their therapeutic efficiency. This review focuses on the applications of DNA aptamer-based AuNPs in several biomedical areas, including anticoagulation, anticancer, antibacterial, and antiviral applications.

show abstract

Synthesis and biological activities of a phosphorodithioate analog of 2′, 5′-oligoadenylate

Abstract: To enhance the resistance of 2-5A (pppA2'p5'A2'p5'A) to degradation by exo-and endonucleases, a phosphorodithioate analog was synthesized using a solid-phase phosphite triester approach with N6-benzoyl-5'--dime-

Cited by 29 publications

References 29 publications

Aptamers in Diagnostics and Treatment of Viral Infections

Aptamers in Diagnostics and Treatment of Viral Infections

Synthetic nanocarriers for the delivery of polynucleotides to the eye

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

Contact Info

Product

Resources

About