Synthesis and bioimmunological efficiency of poly(2-oxazolines) containing a free amino group

Abstract

Novel amphiphilic copolymers on the basis of 2-oxazolines containing a free amino group were prepared. The copolymers were synthesized by the living cationic polymerization of 2-ethyl-2-oxazoline (ETOX) and 2-(4-aminophenyl)-2-oxazoline (APOX). The main goal of this work was the synthesis of water soluble polymer material with the defined number of functional groups necessary for the attachment of proteins and polysaccharides. A high concentration of free amino groups allows immobilization of various biosubstances, e.g. drugs, proteins or polysaccharides. Thermal properties have been studied with respect to the composition of the copolymers. Cytotoxicity and the bioimmunological efficiency of the selected copolymer were studied.

Biocompatibility and Immunocompatibility Assessment of Poly(2-Oxazolines)

“…% aromatic moiety (AEOX10) (synthesis see Fig. 8) has no affect on the growth and proliferation of cells and that the values of cytotoxicity measured in the MTT test were on the same level as in the case of PETOX and PMEOX [27,63]. All results confirmed low cytotoxicity of the polymers prepared by cationic polymerization of 2-alkyl-and 2-aryl-2-oxazolines, which supports their utilization in biomedical applications.…”

“…The protecting group was removed after the polymerization process was completed. Copolymers containing a free amino group were prepared directly by the cationic copolymerization of 2-ethyl-2-oxazoline and 2-(4-aminophenyl)-2-oxazoline [27].…”

Design Strategies for Functionalized Poly(2-oxazoline)s and Derived Materials

“…The amino groups were subsequently reacted with various isothiocyanates, yielding crosslinked hydrogels in the case of bifunctional isothiocyanates. The copolymerization of EtOx with a non-protected amine, namely (NH 2 )PhOx, yielded an amino-bearing copolymer with however very high M w /M values in the range from 2 to 4, which was referred to as side-reactions of the free amino group during the polymerization [198]. The copolymers were found to be biocompatible materials suited for the immobilization of bioactive species.…”

Modern Pharmacological Approaches to Therapies: Substances Tested in Animal Models of Rheumatoid Arthritis

“…Moreover, in the scale of systemic OS parameters, phagocytosis, oxidative burst and metabolic activity of rat granulocytes isolated from peripheral blood were monitored. Flow cytometric analysis was used for these measurements, according to the method published by Kronek et al (2010) and modified for the model of AA (Bauerova et al, 2010a). Interestingly, increased production of ROS by neutrophils recorded by whole blood chemiluminiscence measurements emerged already in an early phase of disease, on the day 7.…”