Synthesis and applications of Rhodamine derivatives as fluorescent probes

Beija, Mariana; Afonso, Carlos A. M.; Martinho, J. M. G.

doi:10.1039/b901612k

Cited by 1,290 publications

(713 citation statements)

References 99 publications

(151 reference statements)

Supporting

Mentioning

699

Contrasting

Unclassified

Order By: Relevance

“…But upon the addition of Cu 2 þ to RBPH in the pH range 1-6 the solution turns pink and becomes highly fluorescent due to the transformation of spiro closed form to open form [21][22][23][24][25][26][27]. The above transformation was achieved by coordination of Cu 2 þ to the 'O' atom of the carbonyl group and 'N' atom of the imido goup of phenyl hydrazide moiety of the probe leads to the formation of the complex and subsequent hydrolysis of this complex leads to the formation of delocalized xanthene conformation of the rhodamine moiety [21,22] (Fig. S1 supporting information) which confirms the formation of rhodamine B through rhodamine-Cu 2 þ complex formation and subsequent hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

“…Xanthene derivatives possess very attractive spectroscopic properties such as large molar extinction coefficient, high fluorescence quantum yield, and visible light excitation as well as long wavelength emission [21][22][23][24][25][26][27]. These derivatives are colorless and non fluorescent, in spiro ring closed form whereas ring-opened forms are colored and have strong fluorescence emissions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

Kempahanumakkagaari

Ramakrishnappa

Malingappa

2014

Journal of Luminescence

View full text Add to dashboard Cite

a b s t r a c tA simple, sensitive and selective fluorescent chemo dosimeter rhodamine B phenyl hydrazide (RBPH) for Cu 2 þ was proposed. This probe is non fluorescent and colorless but exhibits fluorescent enhancement at 580 nm and displayed color change from colorless to pink for Cu 2 þ in the pH range 1-6. Fluorescence microscope experimental results reveals that this chemo sensor is cell permeable and can be used for fluorescence imaging of Cu 2 þ ions in living cells. This probe can detect Cu 2 þ with good linear relationships from 10 to 100 nM with r ¼0.99971 then limit of detection was found to be 0.015 nM with 7 0.91% RSD at 10 nM concentrations.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

Kempahanumakkagaari

Ramakrishnappa

Malingappa

2014

Journal of Luminescence

View full text Add to dashboard Cite

show abstract

“…Highly fluorescent dyes based, for example, on rhodamine,6, 7 fluorescein,3, 8 oxazine,3 coumarin,3 cyanine9, 10 or boron dipyrromethene (BODIPY)11, 12, 13, 14, 15 chromophores, have been extensively explored along these lines. In particular, BODIPY derivatives (Scheme 1 A) became the focus of different fields due to a combination of remarkable photophysical properties, which included very high quantum yields, narrow absorption and emission bands, and high molar absorption coefficients.…”

Section: Introductionmentioning

confidence: 99%

A Three‐Component Assembly Promoted by Boronic Acids Delivers a Modular Fluorophore Platform (BASHY Dyes)

Santos

Rosa

Candeias

et al. 2015

Chemistry A European J

102

View full text Add to dashboard Cite

The modular assembly of boronic acids with Schiff‐base ligands enabled the construction of innovative fluorescent dyes [boronic acid salicylidenehydrazone (BASHY)] with suitable structural and photophysical properties for live cell bioimaging applications. This reaction enabled the straightforward synthesis (yields up to 99 %) of structurally diverse and photostable dyes that exhibit a polarity‐sensitive green‐to‐yellow emission with high quantum yields of up to 0.6 in nonpolar environments. These dyes displayed a high brightness (up to 54 000 m −1 cm−1). The promising structural and fluorescence properties of BASHY dyes fostered the preparation of non‐cytotoxic, stable, and highly fluorescent poly(lactide‐co‐glycolide) nanoparticles that were effectively internalized by dendritic cells. The dyes were also shown to selectively stain lipid droplets in HeLa cells, without inducing any appreciable cytotoxicity or competing plasma membrane labeling; this confirmed their potential as fluorescent stains.

show abstract

“…The presence of iron in body either at higher levels or at lower levels can lead to several problems which eventually disturbs the lipid metabolism and glucose levels 28 . Therefore, quantitative determination of bioactive iron is an important health care challenge.…”

mentioning

confidence: 99%

One-Step Microwave-Assisted Green Synthesis of Luminescent N-Doped Carbon Dots from Sesame Seeds for Selective Sensing of Fe(III)

Roshni¹,

Divya²

2017

Current Science

View full text Add to dashboard Cite

The present study focuses on the green synthesis of nitrogen-doped carbon dots (C.dots) from sesame seeds using microwave pyrolysis method. The C.dots obtained were characterized and extensively studied using transmission electron microscopy (TEM), UV/ visible spectroscopy, fluorescence spectroscopy and Fourier transform infra-red spectroscopy (FTIR) techniques. The results indicated the presence of highly fluorescent, aqueous soluble and significantly photostable C.dots with a quantum yield of 8.02%. The average size distributions of C.dots were found to be 5 nm. These C.dots were effectively applied as a selective sensor for Fe(III) as the fluorescence intensity was significantly quenched with increasing metal concentrations. The limit of detection (LOD) was found to be 2.56 M of Fe(III). This study demonstrates a low cost, environmental friendly and waste recyclable synthetic method for preparation of C.dots and its application as a selective Fe(III) sensor.Keywords: Carbon dots, fluorescence, sesame seeds, microwave pyrolysis, metal ions, quenching.CARBON DOTS (C.dots) are gaining increasing attention within the carbon nanomaterial family due to their varied applications in biological, sensing, optical and electrical fields 1-3 . They are swiftly replacing the conventional semiconductor nanoparticles (Q.dots) which have raised serious health and environmental concerns because of the presence of heavy metals in it 4 . C.dots are spherical, oxygenous carbon nanoparticles with size below 10 nm. C.dots akin to Q.dots show several interesting properties such as excitation-dependent emission, chemical and photostability, enhanced fluorescence quantum yields and the possibility of fine-tuning the sensing properties by surface modification [5][6][7][8][9][10][11] In order to improve the photophysical and photochemical properties of C.dots, hetero-atom doped C.dots are synthesized by different ways. Among them, nitrogendoped C.dots received much attention because of their good biocompatibility and the related broad applications in the areas of bio-imaging, electrocatalysis and sensors 25,26 . However, most of the synthesis methods reported so far have either a long reaction time or a need of extra chemicals for doping. Thus, synthesizing Ndoped C.dots in a cost effective and ecofriendly manner and using them for sensing applications, especially for biologically active and environmentally hazardous metal ions will be highly beneficial.Iron is an essential element for many biological processes 27 . The presence of iron in body either at higher levels or at lower levels can lead to several problems which eventually disturbs the lipid metabolism and glucose levels 28 . Therefore, quantitative determination of bioactive iron is an important health care challenge. Some studies have already been reported in this direction; Qian et al. 29 used the N-doped C.dots for detection of Fe(III). Wang et al. 30 used a wide variety of green carbonaceous precursors like milk, silk, honey, hair, lemon, etc. to detect Fe(III).The pre...

show abstract

Synthesis and applications of Rhodamine derivatives as fluorescent probes

Cited by 1,290 publications

References 99 publications

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

A Three‐Component Assembly Promoted by Boronic Acids Delivers a Modular Fluorophore Platform (BASHY Dyes)

One-Step Microwave-Assisted Green Synthesis of Luminescent N-Doped Carbon Dots from Sesame Seeds for Selective Sensing of Fe(III)

Contact Info

Product

Resources

About