Synthesis and application of quantum dots-based biosensor

Nguyễn, Ngọc Hải; Duong, Thi Giang; Nong, Hanond; Pham, Nam Thang; Đào, Trần Cao; Pham, Thu Nga

doi:10.1088/2043-6262/6/1/015015

Cited by 10 publications

(7 citation statements)

References 16 publications

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“…The size and composition of the nanoparticles are responsible for their unique physical, chemical and optical properties, which arise through quantum confinement effect (Alivisatos, 1996; Rengers et al, 2019). The remarkable properties of QDs, such as broad absorption, narrow and size-dependent emission spectra, resistance to photobleaching, strong luminescence and long luminescent lifetimes (Alivisatos, 1996; Zhou and Ghosh, 2007) allow their use in a number of technology-based applications of high economic, technological and biological value as for example optoelectronics (Faraon et al, 2007), solar cells (Nozik et al, 2010; Muthalif et al, 2019), imaging techniques (Wagner et al, 2019) and quantification of different molecules (Durán-Toro et al, 2014; Nguyen et al, 2015), among others.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

et al. 2019

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Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H 2 S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1 T : megL – (unable to produce MeSH from Met) and mddA – (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL – strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA – strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur source. This work represents the first report that directly associates the production of MeSH with the bacterial synthesis of QDs, thus revealing the importance of different VSCs in the biological generation of metal sulfide nanostructures.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

et al. 2019

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“…However, MPA-capped core/shell TQDs are highly sensitive to pH: fluorescence increases in intensity and slightly shifts toward the longer wavelength side with increasing pH. The decrease of fluorescence intensity with decreasing pH has also been reported for CdSe/ZnS binary quantum dots [14].…”

Section: Resultsmentioning

confidence: 71%

Mechanism to Detect Pesticide Residues in Tealeaves Based on CdZnSe/ZnS Ternary Alloy Quantum Dots

Hải¹,

Yen²,

Giang³

et al. 2015

Comm. in Phys.

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In this report, we present the optical properties of the biosensors fabricated from CdZnSe/ZnS quantum dots. The optical properties such as absorption and emission of the ternary quantum dots before and after coupling with the protein molecules like streptavidine (SA) and acetylcholinesterase enzymes (AChE), to form a biosensor structure, will be presented. In particular, the changes in luminescence intensity according to the pH value of the solution environment containing biosensor have been considered, before and after the presence of pesticides. The changes in luminescence intensity of the biosensor after the presence of pesticide over time from 2 seconds to 26 minutes were also surveyed. We have been carried out the tests to determine the trace amounts of commercial pesticides like Motox 5EC, containing 5% cypermethrin and Tungatin 10 EC, containing 10% abamectin, on the real samples of tealeaves. Some characteristics of the relationship between composition, structure, and special optical properties of ternary alloy quantum dots will also be presented. These studies open up the potential applications of ternary quantum dots for agricultural production.

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“…The remarkable properties of QDs are associated with the nanocrystals’ size and composition [ 11 , 12 ]. They can be effectively tapped for several applications, such as for imaging techniques [ 15 ], solar cells [ 16 , 17 ], optoelectronics [ 18 ], and quantification of different molecules [ 19 , 20 ], among others.…”

Section: Introductionmentioning

confidence: 99%

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

Gallardo-Benavente

Campo-Giraldo

Castro‐Severyn

et al. 2021

Genes

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Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains.

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Synthesis and application of quantum dots-based biosensor

Cited by 10 publications

References 16 publications

Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragi

Mechanism to Detect Pesticide Residues in Tealeaves Based on CdZnSe/ZnS Ternary Alloy Quantum Dots

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

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