Synthesis and Antiviral Activity of 1-Aryl-3-(3,5-Dioxo-4-Azatetracyclo-[5.3.2.02,6.08,10]Dodec-11-EN-4-YL)Ureas

Synthesis and antiviral activity of camphor-based 1,3-thiazolidin-4-one and thiazole derivatives as Orthopoxvirus-reproduction inhibitors

“…The antiviral activity and cytotoxicity of the synthesised derivatives against VV were evaluated using an adapted method. 17 The commercially available agent, Cidofovir, was used as a positive control, and the results are shown in Table 1. The synthesised compounds can be divided into two main clusters according to the type of derivatisation of camphor thiosemicarbazone 1: (a) those derivatives containing thiazole (e.g.…”

Synthesis and Antiviral Activity of Camphene Derivatives against Different Types of Viruses

“…Vaccinia virus (VV), a member of the Orthopoxvirus genus, is a live attenuated virus used worldwide by the WHO in the smallpox vaccine and widely used to find inhibitors of orthopoxviruses [ 21 ]. The antiviral activity and cytotoxicity of the synthesized derivatives against VV (Copenhagen strain) were evaluated by an adapted method in Vero cell culture [ 22 ]. As positive controls, two compounds were used: the commercially available drug Cidofovir and ST-246.…”

“…The concentration of the virus in the samples used in this work was from 5.6 to 6.1 log 10 PFU per ml. For the determination of the cytotoxicity and antiviral activities of agents against the Vaccinia virus, we used the adapted method previously described [ 22 ].…”

Discovery of a New Class of Inhibitors of Vaccinia Virus Based on (−)‐Borneol from Abies sibirica and (+)‐Camphor

“…The antiviral activity and cytotoxicity of the synthesized derivatives against VV were evaluated using an adapted method ( Table ). The commercially available agent Cidofovir was used as a positive control and the results are listed in Table .…”

“…To determine the cytotoxicity and antiviral activities of agents against vaccinia virus (VV), we adapted a method using 96 well plates . The Emax plate spectrophotometer (Molecular Devices, USA) and the SoftMax 4.0 Software (Molecular Devices, USA), which automatically calculated the TC 50 and IC 50 concentrations of the agents, were used to evaluate the results: TC 50 ‐ the substance concentration, at which 50% of cells in uninfected monolayer are destroyed, IC 50 ‐ the substance concentration, at which 50% of cells in infected monolayer are preserved.…”