Synthesis and antioxidant properties of Lycium barbarum polysaccharides capped selenium nanoparticles using tea extract

Abstract

Selenium nanoparticles (SeNPs) have attracted increasing interest over the last decades because of their activities on redox balance in human body. However, the SeNPs tend to aggregate into large clusters, resulting in lower bioactivity, bioavailability and biocompatibility. Surface-capping agents on SeNPs play crucial roles in its stabilization and biological activity. Here, a green synthesis method for the preparation of Lycium barbarum polysaccharides capped SeNPs using green tea extracts as reductants under mild conditions, at room temperature, is reported. The structure, size, morphology and thermal behaviour were analyzed by various characterization techniques. The functionalized nanoparticles demonstrated high antioxidant activity, including DPPH and ABTS free radical scavenging. Moreover, the SeNPs significantly protected the HO-induced PC-12 cell death. Taken together, these results evidence the possible application of these SeNPs as antioxidants food supplement or ingredient and neuroprotective agent.

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

“…Similarly, XRD pattern also revealed the amorphous nature of nSe ( figure 3(e)). The pattern depicted a broad 2θ diffraction peak at 23.30°in accordance with the previouly documented literature [45,48,49]. Raman spectrum of nSe in the range of 150 to 600 cm −1 exhibited a prominent resonance peak at 253 cm −1 owing to Se-Se stretching vibrations, confirming the amorphous nature of nSe ( figure 3(f)) [46,[50][51][52].…”

“…The size distribution histogram of nSe as obtained from TEM indicated the average particle size of nSe was ∼50.02 nm (figure S4). The nSe synthesized previously using other plant extracts showed larger particle sizes such as from Withania somnifera (45-90 nm ) [45], Lycium barbarum (125 nm) [47]. On the other hand, Kokila et al demonstrated smaller size (4-16 nm) of nSe using Diospyrosmontana, but they were stable only upto 15 days [30].…”

“…As compared to TEM, the larger size of nSe was observed in DLS studies because of the presence of extract coating as also evident by TEM analysis. Also, water molecules used as the solvent bound to the nSe surface may increase its size [44,49,54].…”

The Effect of Lycium barbarum Polysaccharides on Pyroptosis-Associated Amyloid β1-40 Oligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage

“…Briefly, Lycium barbarum sourced from Ningxia province of China was first boiled at 80 °C for 30 min and then soaked for 30 min. The resulting soup was subsequently concentrated by the soaking extracting method [ 47 , 48 ]. The technical bulletin stated that the final powder product contained a standardized 3.5% LBP while the remaining ingredients were mostly lactose.…”

Selenium Analysis and Speciation in Dietary Supplements Based on Next-Generation Selenium Ingredients

“…The use of elemental Se in the form of NPs for different biological applications gained a lot of attention lately, because of several proposed advantages compared with other forms of Se, including higher bioavailability [ 98 ], lower toxicity [ 41 , 99 , 100 , 101 ], potential for local delivery of high doses into cancer cells [ 102 ]; possibility to be modified, stabilized and functionalized by various polymers such as chitosan [ 103 , 104 , 105 , 106 ] or other polysaccharides [ 107 , 108 , 109 , 110 , 111 , 112 ], proteins [ 113 , 114 ], or even combinations between them [ 115 , 116 , 117 ], smaller molecules such as polyphenols [ 118 , 119 , 120 , 121 ], sialic acids [ 122 , 123 ], folic acid or folate (as folate–chitosan smart-shell nanocapsules) [ 124 , 125 , 126 ], and other organic acids [ 37 ]. Chemical, physical, or biological methods are used for producing SeNPs [ 37 ], each having its own advantages and disadvantages.…”