“…Our previous study showed the antimicrobial properties of our Ag-NPs with Ag-carbon σ-bonds against Staphylococcus aureus 16) . However, new properties of nanomaterials may also induce deleterious effects on living organs and cells, so the toxic potential of these materials must be methodically evaluated.…”
Section: Introductionmentioning
confidence: 79%
“…The absorption peaks also indicate that the nanoparticles are dispersible in water. The X-ray diffraction and X-ray photoelectron spectroscopy data characterizing these particles were described in our previous study 16) . Figure 3 shows the effects of Ag-NPs on cell viability in RAW264.7 and MC3T3-E1 cells.…”
Section: Resultsmentioning
confidence: 99%
“…All procedures for synthesis of Ag-NPs were conducted according to previously reported methods 16) . A schematic illustration of the synthesized Ag-NPs is shown in Fig.…”
Section: Size Distribution and Uv-vis Measurement Of Ag-npsmentioning
With the continuous progress in nanomaterial development for biomedicine, the potential cytotoxicity of nanoparticles is drawing more attention and concern for clinical applications. The purpose of this study was to evaluate biological responses of new waterdispersible silver nanoparticles (Ag-NPs) stabilized by Ag-C σ-bonds in cultured murine macrophages (RAW264.7) and osteoblastlike cells (MC3T3-E1) using cell viability and morphological analyses. For RAW264.7, Ag-NPs seemed to induce cytotoxicity that was dependent on the Ag-NP concentration. However, no cytotoxic effects were observed in the MC3T3-E1 cell line. In microscopic analysis, Ag-NPs were taken up by MC3T3-E1 cells with only minor cell morphological changes, in contrast to RAW264.7 cells, in which particles aggregated in the cytoplasm and vesicles. The ability of endocytosis of macrophages may induce harmful effects due to expansion of cell vesicles compared to osteoblast-like cells with their lower uptake of Ag-NPs.
“…Our previous study showed the antimicrobial properties of our Ag-NPs with Ag-carbon σ-bonds against Staphylococcus aureus 16) . However, new properties of nanomaterials may also induce deleterious effects on living organs and cells, so the toxic potential of these materials must be methodically evaluated.…”
Section: Introductionmentioning
confidence: 79%
“…The absorption peaks also indicate that the nanoparticles are dispersible in water. The X-ray diffraction and X-ray photoelectron spectroscopy data characterizing these particles were described in our previous study 16) . Figure 3 shows the effects of Ag-NPs on cell viability in RAW264.7 and MC3T3-E1 cells.…”
Section: Resultsmentioning
confidence: 99%
“…All procedures for synthesis of Ag-NPs were conducted according to previously reported methods 16) . A schematic illustration of the synthesized Ag-NPs is shown in Fig.…”
Section: Size Distribution and Uv-vis Measurement Of Ag-npsmentioning
With the continuous progress in nanomaterial development for biomedicine, the potential cytotoxicity of nanoparticles is drawing more attention and concern for clinical applications. The purpose of this study was to evaluate biological responses of new waterdispersible silver nanoparticles (Ag-NPs) stabilized by Ag-C σ-bonds in cultured murine macrophages (RAW264.7) and osteoblastlike cells (MC3T3-E1) using cell viability and morphological analyses. For RAW264.7, Ag-NPs seemed to induce cytotoxicity that was dependent on the Ag-NP concentration. However, no cytotoxic effects were observed in the MC3T3-E1 cell line. In microscopic analysis, Ag-NPs were taken up by MC3T3-E1 cells with only minor cell morphological changes, in contrast to RAW264.7 cells, in which particles aggregated in the cytoplasm and vesicles. The ability of endocytosis of macrophages may induce harmful effects due to expansion of cell vesicles compared to osteoblast-like cells with their lower uptake of Ag-NPs.
“…Toxic metals have the potential to leach when their chemical structure is changed under special conditions in vivo and in vitro , the use of the stronger σ‐bonds between the metal and carbon (compared with coordinating bonds) might prevent the chemical changes and the leaching of core metals, resulting in good stability in water. Stable metal NPs are therefore the subject of growing interest for applications in the medical field, because of their novel characteristics …”
Nanometals are currently receiving considerable attention for industrial and biomedical applications, but their potentially hazardous and toxic effects have not been extensively studied. This study evaluated the biological responses of novel water-dispersible gold (Au-NPs) and silver nanoparticles (Ag-NPs) stabilized by Au-C or Ag-C σ-bonds in cultured macrophages (RAW264.7), via analysis of the cell viability, the integrity of the plasma membrane, and the inflammatory and morphological properties. The cultured RAW264.7 was exposed to metal-NPs at various concentrations. The Ag-NPs showed cytotoxicity at high NP concentrations, but the cytotoxic effects of the Au-NPs were smaller than those of the Ag-NPs. For the microscopic analysis, both types of particles were internalized into cells, the morphological changes in the cells which manifested as an expansion of the vesicles' volume, were smaller for the Au-NPs compared with the Ag-NPs. For the Ag-NPs, the endocytosis abilities of the macrophages might have induced harmful effects, because of the expansion of the cell vesicles. Although an inflammatory response was observed for both the Au- and Ag-NPs, the harmful effects of the Au-NPs were smaller than those of the Ag-NPs, with minor morphological changes observed even after internalization of the NPs into the cells.
“…Furthermore, the bactericidal properties of the silver NPs also depend on their stability and solubility in the growth medium, which renders greater retention time for the interaction between bacteria and NPs. 27,28 In general, the stabilization of NPs is achieved via surface functionalization of the NPs, using various capping agents that bind to the surface of the NPs and inhibit their aggregation by enhancing their stability and water solubility. 29 Moreover, it has also been suggested that the antibacterial activity of the silver NPs can be significantly enhanced by the proper choice and control of stabilizing agents.…”
The antibacterial properties of nanoparticles (NPs) can be significantly enhanced by increasing the wettability or solubility of NPs in aqueous medium. In this study, we investigated the effects of the stabilizing agent on the solubility of silver NPs and its subsequent effect on their antimicrobial activities. Silver NPs were prepared using an aqueous solution of
Pulicaria glutinosa
plant extract as bioreductant. The solution also acts as a capping ligand. During this study, the antimicrobial activities of silver NPs, as well as the plant extract alone, were tested against
Escherichia coli
,
Pseudomonas aeruginosa
,
Staphylococcus aureus
, and
Micrococcus luteus
. Silver NPs were prepared with various concentrations of the plant extract to study its effect on antimicrobial activity. Interestingly, various concentrations of
P. glutinosa
extract did not show any effect on the growth of tested bacteria; however, a significant effect on the antimicrobial property of plant extract capped silver NPs (Ag-NPs-PE) was observed. For instance, the half maximal inhibitory concentration values were found to decrease (from 4% to 21%) with the increasing concentrations of plant extract used for the synthesis of Ag-NPs-PE. These results clearly indicate that the addition of
P. glutinosa
extracts enhances the solubility of Ag-NPs-PE and, hence, increases their toxicity against the tested microorganisms.
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