Synthesis and Anti-Staphylococcal Activity of TiO&lt;SUB&gt;2&lt;/SUB&gt; Nanoparticles and Nanowires in &lt;I&gt;Ex Vivo&lt;/I&gt; Porcine Skin Model

Nataraj, Namrata; Anjusree, G. S.; Madhavan, Asha Anish; P, Priyanka; Sankar, Deepthi; Nisha, N.; Lakshmi, S.; Jayakumar, R.; Balakrishnan, Avinash; Biswas, Raja

doi:10.1166/jbn.2014.1756

Cited by 45 publications

(26 citation statements)

References 25 publications

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“…121 In addition, the potential of the bacterial cell membrane plays an important role in cell communication, which has a close relationship with apoptosis. For example, the disruptive effect of TiO 2 NPs on the potential of the bacterial cell membrane was measured by fluorescence microscopy, 122 and changes in the fluorescence intensity of the cytoplasm were observed when altering the potential of the cell membrane.…”

mentioning

confidence: 99%

The antimicrobial activity of nanoparticles: present situation and prospects for the future

Wang¹,

Hu²,

Shao³

2017

IJN

2,929

1,792

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Nanoparticles (NPs) are increasingly used to target bacteria as an alternative to antibiotics. Nanotechnology may be particularly advantageous in treating bacterial infections. Examples include the utilization of NPs in antibacterial coatings for implantable devices and medicinal materials to prevent infection and promote wound healing, in antibiotic delivery systems to treat disease, in bacterial detection systems to generate microbial diagnostics, and in antibacterial vaccines to control bacterial infections. The antibacterial mechanisms of NPs are poorly understood, but the currently accepted mechanisms include oxidative stress induction, metal ion release, and non-oxidative mechanisms. The multiple simultaneous mechanisms of action against microbes would require multiple simultaneous gene mutations in the same bacterial cell for antibacterial resistance to develop; therefore, it is difficult for bacterial cells to become resistant to NPs. In this review, we discuss the antibacterial mechanisms of NPs against bacteria and the factors that are involved. The limitations of current research are also discussed.

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mentioning

confidence: 99%

The antimicrobial activity of nanoparticles: present situation and prospects for the future

Wang¹,

Hu²,

Shao³

2017

IJN

2,929

1,792

View full text Add to dashboard Cite

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“…Extent of hemolysis was determined according to previously described method [21]. Briefly 1 ml blood was diluted 10 times with Ringer buffer.…”

Section: Determination Of Hemolysismentioning

confidence: 99%

Combinatorial Effect of D-Aminoacids and Tetracycline Against Pseudomonas Aeruginosa Biofilm

Jayalekshmi

Harikrishnan

Sali

et al. 2016

Int J Pharm Pharm Sci

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Objective: The present study attempted to evaluate the anti-biofilm activity of D-amino acids (D-AAs) on Pseudomonas aeruginosa and determine if the combination of D-AAs with tetracycline enhances the anti-biofilm activity in vitro and ex vivo. Methods:Different D-AAs were tested for antibiofilm activity against wild type P. aeruginosa PAO1 and two multidrug resistant P. aeruginosa clinical strains in the presence of sub inhibitory concentrations of tetracycline using crystal violet microtitre plate assay. Results were further validated using in vitro wound dressing and ex vivo porcine skin models followed by cytotoxicity and hemocompatibility studies.Results: D-tryptophan (5 mmol) showed 61 % reduction in biofilm formation of P. aeruginosa. Interestingly combinatorial effect of 5 mmol Dtryptophan and 0.5 minimum inhibitory concentration (MIC) (7.5µg/ml) tetracycline showed 90% reduction in biofilm formation. 5 mmol Dmethionine shows 28 % reduction and combination with tetracycline shows 41% reduction in biofilm formation of P. aeruginosa. D-leucine and Dtyrosine alone or in combination with tetracycline did not show significant anti-biofilm activity. D tryptophan-tetracycline combination could reduce 80 % and 77 % reduction in biofilm formation in two multi drug resistant P. aeruginosa clinical strains. D-tryptophan-tetracyclinecombination could also reduce 76% and 66% reduction in biofilm formation in wound dressing model and porcine skin explant respectively. The cytotoxicity and hemocompatibility studies did not show significant toxicity when this combination was used. Conclusion:The results established the potential therapeutic application of D-tryptophan alone or in combination with tetracycline for treating biofilm associated clinical problems caused by P. aeruginosa.

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“…The antibacterial effects of Ag-based NWs (Holtz et al 2010(Holtz et al , 2012Jiang et al 2012;Liu et al 2013;Schoen et al 2010;Singh et al 2014;Tamboli et al 2012;Tang et al 2014;Visnapuu et al 2013;Zhang et al 2007), Si NWs (Lv et al 2010), ZnO NWs (Kılıç and Omay 2014;Wu et al 2011), MgO NWs (AlHazmi et al 2012, Mn 2 O 3 NWs (Hassan et al 2012), Tibased NWs (Nataraj et al 2014;Shang et al 2010), and CdO NWs (Kumar and Ojha 2013) were evaluated. Test subjects included the pathogenic bacteria species Escherichia coli, Bacillus subtilis, Staphylococcus aureus, …”

Section: Toxicity Of Nanowires To Bacteriamentioning

confidence: 99%

“…Nataraj et al (2014) NWs were also observed to disrupt E. coli cell membranes and to cause leakage of intracellular contents in a study that used TEM analysis (Hassan et al 2012). According to Zhang et al (2007), the effects of Ag NWs on bacteria may be similar to those of Ag, which has strong antibacterial activity and inactivates bacterial proteins by interacting with the -SH group in the bacterial protein molecules.…”

Section: Toxic Mechanism Of Nanowiresmentioning

confidence: 99%

“…Nanowires have potential applications in the biomedical and clinical industries (Brammer et al 2009;Johansson et al 2010;Nataraj et al 2014;Singh et al 2013), as antibacterial agents (Fellahi et al 2013;Holtz et al 2010Holtz et al , 2012Wu et al 2011), as sensors of metals (Luo et al 2009;Mu et al 2007), in solar cells (Garnett and Yang 2010;Hamilton et al 2009), in the removal of metals (Jia et al 2013;Youssef and Malhat 2014), and in energy storage (Chen et al 2009;Tiwari et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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A review of the ecotoxicological effects of nanowires

Kwak

2014

Int. J. Environ. Sci. Technol.

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We briefly reviewed the existing research on the ecotoxicity of nanowires and suggested directions for further study. Nanowires are technological innovations that can benefit humans. However, it is important to consider the effects of nanowires on the environment. Only a few studies have reported acute and chronic ecological toxicity of nanowires on aquatic and terrestrial organisms, and limited research papers have reported antibacterial effects of nanowires. It is assumed that nanowires have a toxic mechanism similar to that of nanoparticles or ions, but the mechanism remains unknown because so little research has been conducted on the ecological toxicity of nanowires. More in-depth assessments of the chronic toxicity, bioavailability, cytotoxicity, and genotoxicity of nanowires on various species are needed.

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Synthesis and Anti-Staphylococcal Activity of TiO<SUB>2</SUB> Nanoparticles and Nanowires in <I>Ex Vivo</I> Porcine Skin Model

Cited by 45 publications

References 25 publications

The antimicrobial activity of nanoparticles: present situation and prospects for the future

The antimicrobial activity of nanoparticles: present situation and prospects for the future

Combinatorial Effect of D-Aminoacids and Tetracycline Against Pseudomonas Aeruginosa Biofilm

A review of the ecotoxicological effects of nanowires

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