Syntheses of cross-linked polymeric superparamagnetic beads with tunable properties

Maurizi, Lionel; Sakulkhu, Usawadee; Crowe, Lindsey A.; Dao, Vanessa Mai; Leclaire, Nicolas; Vallée, Jean‐Paul; Hofmann, Heinrich

doi:10.1039/c3ra48004f

Cited by 14 publications

(17 citation statements)

References 32 publications

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“…One possibility would be to use the PVA coating properties to cross-link the polymer on the surface of the SPION or to functionalize them with reactive groups to target specific diseases. 80 Of course, these new modifications will need further in vitro studies. However, once a suitable formulation has been established, it may facilitate prevention of joint damage arising from RA.…”

Section: Discussionmentioning

confidence: 99%

Effects of PVA coated nanoparticles on human immune cells

Strehl¹,

Gaber²,

Maurizi³

et al. 2015

IJN

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Nanotechnology provides new opportunities in human medicine, mainly for diagnostic and therapeutic purposes. The autoimmune disease rheumatoid arthritis (RA) is often diagnosed after irreversible joint structural damage has occurred. There is an urgent need for a very early diagnosis of RA, which can be achieved by more sensitive imaging methods. Superparamagnetic iron oxide nanoparticles (SPION) are already used in medicine and therefore represent a promising tool for early diagnosis of RA. The focus of our work was to investigate any potentially negative effects resulting from the interactions of newly developed amino-functionalized amino-polyvinyl alcohol coated (a-PVA) SPION (a-PVA-SPION), that are used for imaging, with human immune cells. We analyzed the influence of a-PVA-SPION with regard to cell survival and cell activation in human whole blood in general, and in human monocytes and macrophages representative of professional phagocytes, using flow cytometry, multiplex suspension array, and transmission electron microscopy. We found no effect of a-PVA-SPION on the viability of human immune cells, but cytokine secretion was affected. We further demonstrated that the percentage of viable macrophages increased on exposure to a-PVA-SPION. This effect was even stronger when a-PVA-SPION were added very early in the differentiation process. Additionally, transmission electron microscopy analysis revealed that both monocytes and macrophages are able to endocytose a-PVA-SPION. Our findings demonstrate an interaction between human immune cells and a-PVA-SPION which needs to be taken into account when considering the use of a-PVA-SPION in human medicine.

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Section: Discussionmentioning

confidence: 99%

Effects of PVA coated nanoparticles on human immune cells

Strehl¹,

Gaber²,

Maurizi³

et al. 2015

IJN

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“…SPIONs grafted with PEG do not show any cytotoxicity via MTT assay at a concentration up to 270 µgFe/mL, 24 h after incubation with RAW 264.7 and HepG2 cell lines [5]. Polyvinyl alcohol (PVA) is also used to stabilize SPIONs and do not have any cytotoxic effects (MTS assay) for a concentration of 800 μgFe/mL 24 h after incubation with RAW 264.7 cells [18]. Furthermore, PVA coated SPIONs do not significantly activate or influence human T helper cells and have a negligible influence on T cell apoptosis at a concentration of 100 μgSPIONs/mL after 72 h [19].…”

Section: Cytotoxicity Of Spionsmentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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The lack of toxicological data on nanomaterials makes it difficult to assess the risk related to their exposure, and as a result further investigation is required. This chapter presents the synthesis of controlled oxide nanoparticles followed by the evaluation of their safety profile or toxicity (iron, titanium and zinc oxides). The controlled surface chemistry, dispersion in several media, morphology and surface charge of these nanoparticles are presented (transmission electron microscopy, dynamic light scattering, zeta potential, X-ray photoelectron spectroscopy). Classical cytotoxic and cellular uptake studies on different cancer cell lines from liver, prostate, heart, brain and spinal cord are discussed. The incidence of nanoparticles on biogenesis and activity of cell organelles is also highlighted, as well as their biodistribution in animal models. The acute toxicity on zebrafish embryo model is also presented. Finally, the stress is put on the influence and the necessity of controlling the protein corona, a layer of plasma proteins physically adsorbed at the surface of such nanoparticles as a result of their presence in the bloodstream (or relevant biological fluids).

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“…Naked SPIONs (maghemite phase of 8 nm±2 nm crystallite's diameter) were preliminary coated with a mixture of polyvinyl alcohol (PVA) as described in previous works [31,32] (these PVA-coated SPIONs will simply be called SPIONs for the remainder of this document). Their hydrodynamic radius R H in a PBS buffer (phosphate buffered saline, Sigma-Aldrich Chemie) as measured by OCCS yielded a mean diameter of 42.0 nm with a standard deviation of 4.7 nm (20 measurements lasting 20 seconds each; line rate of 50 kHz with an exposure time of 18 µs; illumination power of 5 mW).…”

Section: Protein Adsorption On Spionsmentioning

confidence: 99%

Visible light optical coherence correlation spectroscopy

Broillet¹,

Szlag²,

Bouwens³

et al. 2014

Opt. Express

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Abstract:Optical coherence correlation spectroscopy (OCCS) allows studying kinetic processes at the single particle level using the backscattered light of nanoparticles. We extend the possibilities of this technique by increasing its signal-to-noise ratio by a factor of more than 25 and by generalizing the method to solutions containing multiple nanoparticle species. We applied these improvements by measuring protein adsorption and formation of a protein monolayer on superparamagnetic iron oxide nanoparticles under physiological conditions.

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Syntheses of cross-linked polymeric superparamagnetic beads with tunable properties

Abstract: Novel, fast and reproducible way to obtain magnetic silica beads by PVA, silica and iron oxide nanoparticles cross-linking process.

Cited by 14 publications

References 32 publications

Effects of PVA coated nanoparticles on human immune cells

Effects of PVA coated nanoparticles on human immune cells

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Visible light optical coherence correlation spectroscopy

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