Syntheses of a new class of phenyl butyraldehyde-derived amines with in vitro trypanocide activities

Rational design of nitrofuran derivatives: Synthesis and valuation as inhibitors of Trypanosoma cruzi trypanothione reductase

“…TR activity was measured by monitoring DTNB reduction at 405 nm (ε ¼ 13.8 mM À1 cm À1 at 405 nm) by means of a coupled assay that guaranteed the oxidation of T(SH) 2 [41]. All enzyme assays were performed at 30 C, in a final volume of 50 ml, and using a Multiskan Ascent one channel vertical light-path filter photometer (Thermo Electron Co.).…”