Syntheses of (5,8-l3C2)- and (1,12-l3C2)Spermine Using Potassium (l3C)Cyanide as the 13C Source.

Simultaneous Determination of Endogenous and Orally Administered 15N-Labeled Polyamines in Rat Organs

“…Standard polyamines (putrescine ⅐ 2 HCl, spermidine ⅐ 3 HCl, and spermine ⅐ 4 HCl) were purchased from Sigma (Japan). [1,[4][5][6][7][8][9][10][11][12][13][14][15] .0 mmol) and 1,2-dibromoethane (1.3 g, 7.0 mmol) were refluxed for 5 h in 5 mL of 70% EtOH according to the method developed for 13 C-labeled spermine (16). Acetone was then added to the reaction mixture to precipitate potassium bromide, and the filtrate was evaporated to dryness in vacuo at 30°C to obtain [ 13 C, 15 N]succinodinitrile (270 mg, 3.2 mmol).…”

Novel Synthetic Polyamines Are Effective in the Treatment of Experimental Microsporidiosis, an Opportunistic AIDS-Associated Infection

“…The three pentamidine analogues (SL-11134, SL-11136, and SL-11137) were obtained by modifying known procedures. Thus, 1,5-bis(p-cyanobenzyl)-diaminobutane, 1,6-bis(p-cyanobenzyl)-diaminopentane, and 1,7-bis(p-cyanobenzyl)-diaminohexane were obtained by previously described procedures (33). The corresponding amidines were prepared by the following procedure.…”

Cadaverine turnover in soybean seedlings using 15N-labelled lysine and cadaverine

“…15 N-Cadaverine was produced according to the method described for 15 N-putrescine synthesis [28], but using 1,5-dibromopentane instead of 1,4-dibromobutane. The internal standard 13 C, 15 N-cadaverine was prepared utilizing 1,3-dibromopropane and potassium [ 13 C, 15 N]cyanide using the method of Samejima et al [27], with small modifications. 4.2.…”