Syntheses, characterization and DNA-binding study of chiral complexes ΔΔ- and ΛΛ-[Ru(bpy)2(bdptb)Ru(bpy)2]4+

DNA Binding and Photocleavage Properties, Cellular Uptake and Localization, and in-Vitro Cytotoxicity of Dinuclear Ruthenium(II) Complexes with Varying Lengths in Bridging Alkyl Linkers

“…Similar to the previously reported behavior of 2 , the luminescence intensity of the cell population increased dramatically after the cells were incubated for 24 h with 20 μM of 1 and 3 under a fluorescence microscope compared to the autofluorescence of untreated cells . Because of good luminescent stability of 1 – 3 aqueous solutions, the cellular uptake properties of 1 – 3 can be conveniently studied using confocal microscopy.…”

“…The absorption spectra of complexes 1 and 3 in the absence and presence of increasing concentrations of calf thymus DNA (CT-DNA) are shown in Figure and Figure S1, respectively. The spectral behaviors of 1 and 3 are very similar to those of 2 with obvious hypochromism, H % (as defined by H % ≅ 100­( A free – A bound )/ A free ), and bathochromism, as indicated by Δλ (Δλ = λ bound – λ free ). Upon increasing concentrations of CT-DNA to the constant spectra (saturation), the H % (Δλ) values at ∼280 nm were found to be 70 (4 nm), 73 (5 nm), and 63% (3 nm) for 1 , 2 , and 3 , respectively; the H % (Δλ) values at ∼460 nm were found to be 47 (8 nm), 51 (6 nm), and 36% (5 nm) for 1 , 2 , and 3 , respectively.…”

“…Upon increasing concentrations of CT-DNA to the constant spectra (saturation), the H % (Δλ) values at ∼280 nm were found to be 70 (4 nm), 73 (5 nm), and 63% (3 nm) for 1 , 2 , and 3 , respectively; the H % (Δλ) values at ∼460 nm were found to be 47 (8 nm), 51 (6 nm), and 36% (5 nm) for 1 , 2 , and 3 , respectively. It was reported that dinuclear Ru complex-based DNA groove binders of 4 , 5 , and 6 and partial intercalators of 7 , 8 , and 9 could exhibit obvious bathochromic shifts and hypochromisms in their visible absorption and/or UV bands upon their binding to the DNA, in which 4 , for example, had H % (Δλ) values of ∼20% (+20 nm). Thus, the evident spectral changes observed for 1 – 3 suggest a strong interaction between the complexes and the DNA, but groove binding and partial intercalation could not be excluded.…”

“…Herein, we report the synthesis and characterization of two analogous Ru­(II) complexes ( 1 and 3) with ten- and three-methylene in their bridging linkers (see Scheme ), respectively, and the effects of the linker lengths on their DNA binding and photocleaving, in vitro cytotoxic, and cellular uptake and localization properties. These properties are compared to those of some previously reported representative complexes (see Scheme for their molecular structures). − …”

Synthesis, Structural and Optical Properties of TOPO and HDA Capped Cadmium Sulphide Nanocrystals, and the Effect of Capping Ligand Concentration

“…The 1 H-NMR spectra of the complex show correct proton peaks and multiplicities for the allyl ligand. The peaks were assigned based on similar ligands [29,30], and the coordination of the ligand to the metal can be assumed by the general chemical shift differences of the allylic protons as compared to the free ligands [31]. The peaks in the range 4.43-5.25 ppm are ascribed to the allylic protons, while the peaks in the range 5.82-5.95 ppm are characteristic of the vinylic protons.…”