Synthèse du linolènate et de l'arachidonate de cholestéryle doublement marqués

Abstract

The transesterification method used by MAHADEVAN and LUNDBERG for cholesterol esters synthesis, following the scheme cholesterol acetate + fatty acid methyl ester + cholesterol ester + methyl acetate, has been adapted to the microscale (1 to 10 pM) imposed by the use of labelled molecules of high specific activity.This method is particularly suitable for the synthesis of polyethylenic fatty acid cholesterol esters : it has unable us to obtain the following esters :414C and 7a3H cholesteryl linolenate and arachidonate, cholesteryl linolenate U14C and arachidonate (5-6, 8-9, 11-12, 14-15)3H; the mixture of these two types of esters of high specific activity give rise to series of doubly labelled esters, at the user's convenience. RESUME La methode de transesterification utilisee par MAHADEVAN et LUNDBERG pour synthetiser des esters du cholestCrol selon : acCtate de cholestCryle + ester mtthylique d'acides gras -> ester du cholesterol + acCtate de mtthyle, a Ct C adaptee aux microquantitks (1 a 10 pM) de 1'Cchelle imposte par l'emploi de * R e p le 29 d h m b r e 1964. Actuellement Attach6 de Recherches a 1'1. N. S. E. R. M Cf. Cgalement (( Recent Studies on the metabolism of cholesterol esters n (DEWITT and S. GOODMAN) et (( Origin and role of plasma cholesterol esters P (L. GIDEZ) in Gordon Research Conferences. 15-19 juin 1964.