Synoviocyte neotissues towards in vitro meniscal tissue engineering

Warnock, Jennifer J.; Duesterdieck-Zellmer, Katja F.; Bobe, Gerd; Baltzer, Wendy I.; Ott, Jesse

doi:10.1016/j.rvsc.2013.07.026

Cited by 7 publications

(13 citation statements)

References 55 publications

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“…Consistent with prior studies (Warnock et al 2013), all oaTSB and nTSB in the present study were negative for any macrophages, which have been reported to contaminate human osteoarthritic synoviocyte monolayer cultures and reduce in vitro chondrogenic activity (Pei et al, 2008a) by contributing to the inflammatory milieu. In the present study, 4 passages and long term culture as TSB likely eliminated any non-adherent cells, including synovial macrophages (Krey et al, 1976).…”

Section: Reviewing Manuscriptsupporting

confidence: 92%

“…In the present study, staining for ASM was positively associated with the formation of circular defects, indicating that the ECM was not strong enough to prevent tears from forming during ASM-mediated self-tensioning (Kambic et al, 2000;Vickers et al, 2004;Warnock et al, 2013).…”

Section: Reviewing Manuscriptcontrasting

confidence: 56%

“…At the 4th passage, cells from each joint were transferred into eight 150cm 2 flasks and allowed to become hyperconfluent cell sheets, defined as cells overlapping each other in greater than 100% confluency. TSB were then made as previously described (Warnock et al, 2013). Briefly, hyperconfluent cell sheets were dislodged off the flask floors (Ando et al, 2008), and each sheet was wrapped over 2.0 cm diameter, 22ga cerclage wire hoops in three layers, with approximately 0.5 N of tension to avoid tearing, to synthesize TSB.…”

Section: Cell Culturementioning

confidence: 99%

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Peer Review #1 of "Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs (v0.1)"

Shvartsman¹

2014

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Meniscal tears are a common cause of stifle lameness in dogs. Use of autologous synoviocytes from the affected stifle is an attractive cell source for tissue engineering replacement fibrocartilage. However, the diseased state of these cells may impede in vitro fibrocartilage formation. Synoviocytes from 12 osteoarthritic ("oaTSB") and 6 normal joints ("nTSB") were cultured as tensioned bioscaffolds and compared for their ability to synthesize fibrocartilage sheets. Gene expression of collagens type I and II were higher and expression of interleukin-6 was lower in oaTSB versus nTSB. Compared with nTSB, oaTSB had more glycosaminoglycan and alpha smooth muscle staining and less collagen I and II staining on histologic analysis, whereas collagen and glycosaminoglycan quantities were similar. In conclusion, osteoarthritic joint-origin synoviocytes can produce extracellular matrix components of meniscal fibrocartilage at similar levels to normal joint-origin synoviocytes, which makes them a potential cell source for canine meniscal tissue engineering.

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Section: Reviewing Manuscriptsupporting

confidence: 92%

Section: Reviewing Manuscriptcontrasting

confidence: 56%

Section: Cell Culturementioning

confidence: 99%

See 1 more Smart Citation

Peer Review #1 of "Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs (v0.1)"

Shvartsman¹

2014

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“…Multiple studies reported a significant increase in miR‐146a expression in IL‐1β‐stimulated and OA chondrocytes and synoviocytes, which was observed in our study not only in TNF‐α and IL‐1β‐stimulated chondrocytes and synoviocytes, but also in TNF‐α and IL‐1β‐stimulated meniscus cells. MiR‐140, regulator of aggrecanase‐2, was significantly decreased in both TNF‐α and IL‐1β‐stimulated chondrocytes and meniscus cells, but not in stimulated synoviocytes, which can be attributed to the fact that meniscus and cartilage tissues naturally contain more aggrecan than synoviocytes . Expression of miR‐27b, direct regulator of MMP‐13, was significantly down‐regulated in all cell types by both TNF‐α and IL‐1β.…”

Section: Discussionmentioning

confidence: 95%

Inflammatory cytokines induce specific time- and concentration-dependent MicroRNA release by chondrocytes, synoviocytes, and meniscus cells

et al. 2015

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In knee osteoarthritis (OA), concentrations of interleukin (IL)-1b and tumor necrosis factor (TNF)-a increase in joint tissues and synovial fluid which incite a catabolic cascade and further the progression of OA. Several microRNAs (miRNA) have been associated with apoptosis (miR-16), inflammation (miR-22, miR-146a), and matrix degradation (miR-140, miR-27b) in developed OA or its symptoms. In this study, the time-and concentration-dependent nature of cellular and extracellular miRNAs in synoviocytes, meniscus cells, and chondrocytes as influenced by inflammatory cytokines was investigated. For time-dependent studies, three cell types were stimulated with 10 ng/ml IL-1b or 50 ng/ml TNF-a for 8, 16, and 24 h. For concentration-dependent studies, chondrocytes were stimulated with a higher level of IL-1b (20 ng/ml) or TNF-a (100 ng/ml) for 8 h. Cellular and extracellular expressions of miR-22, miR-16, miR-146a, miR-27b, and miR-140 were analyzed by RT-PCR. Time-dependent cellular miRNA expressions were similar across the three cell types with miR-146a significantly up-regulated and miR-27b significantly down-regulated at all time points. However, chondrocytes exhibited a unique extracellular miRNA profile with an increased release rate of miR-27b at 24 h. Our findings support further research into the characterization of miRNAs in synovial fluid for the development of early detection strategies of OA or cartilage injury. ß

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“…Autologous, osteoarthritic joint—origin synovium has been investigated as a cell source for fibrocartilage tissue engineering in dogs, because of its abundance and ease of harvest during clinically required surgical procedures ( Warnock et al, 2012 ). In vitro , cultured osteoarthritic joint—origin canine synovial membrane cells are plastic adherant and fibroblast—like, and contain populations of cells that can undergo chondrogenesis ( Warnock et al, 2011 ; Warnock et al, 2013 ), suggesting the presence of mesenchymal stem cells. In vivo synovium also has the ability to form fibrocartilage ECM ( Smith et al, 2012 ; Tienen et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs

Warnock

Bobe

Duesterdieck-Zellmer

2014

PeerJ

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Meniscal tears are a common cause of stifle lameness in dogs. Use of autologous synoviocytes from the affected stifle is an attractive cell source for tissue engineering replacement fibrocartilage. However, the diseased state of these cells may impede in vitro fibrocartilage formation. Synoviocytes from 12 osteoarthritic (“oaTSB”) and 6 normal joints (“nTSB”) were cultured as tensioned bioscaffolds and compared for their ability to synthesize fibrocartilage sheets. Gene expression of collagens type I and II were higher and expression of interleukin-6 was lower in oaTSB versus nTSB. Compared with nTSB, oaTSB had more glycosaminoglycan and alpha smooth muscle staining and less collagen I and II staining on histologic analysis, whereas collagen and glycosaminoglycan quantities were similar. In conclusion, osteoarthritic joint—origin synoviocytes can produce extracellular matrix components of meniscal fibrocartilage at similar levels to normal joint—origin synoviocytes, which makes them a potential cell source for canine meniscal tissue engineering.

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Synoviocyte neotissues towards in vitro meniscal tissue engineering

Cited by 7 publications

References 55 publications

Peer Review #1 of "Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs (v0.1)"

Peer Review #1 of "Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs (v0.1)"

Inflammatory cytokines induce specific time- and concentration-dependent MicroRNA release by chondrocytes, synoviocytes, and meniscus cells

Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs

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