Synovial incorporation of polyacrylamide hydrogel after injection into normal and osteoarthritic animal joints

Christensen, Lise; Camitz, L.; Illigen, K.E.; Hansen, M. Lock; Sarvaa, R.; Conaghan, Philip G.

doi:10.1016/j.joca.2016.07.007

Cited by 19 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A 4 wt% pAAm hydrogel solution was injected into six healthy equine fetlock joints and was shown to be on the surface of the synovium 7 days after injection, and remained visible in the histology of one synovial membrane 56 days after being injected 32 . Transmission electron micrographs of the synovial membrane 7 days and 56 days after injection revealed the presence of the gel in the interstitial spaces as well as being phagocytosed by synoviocytes 32,33 . In another study, histological analysis of four rabbit knee joints and 13 osteoarthritic equine joints treated with a 2.5 wt% pAAm hydrogel solution showed the presence of the pAAm on the surface of the synovium as well as in the synovial folds of the tissue for up to 14 days after injection 33 .…”

Section: Discussionmentioning

confidence: 91%

“…32 Transmission electron micrographs of the synovial membrane 7 days and 56 days after injection revealed the presence of the gel in the interstitial spaces as well as being phagocytosed by synoviocytes. 32,33 In another study, histological analysis of four rabbit knee joints and 13 osteoarthritic equine joints treated with a 2.5 wt% pAAm hydrogel solution showed the presence of the pAAm on the surface of the synovium as well as in the synovial folds of the tissue for up to 14 days after injection. 33 Such localization effects at the surface of the cartilage and synovial membrane may be clinically beneficial due to the increased residence time of the gel in the joint.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Polyacrylamide hydrogel lubricates cartilage after biochemical degradation and mechanical injury

Vishwanath

McClure²,

Bonassar

2022

Journal Orthopaedic Research

View full text Add to dashboard Cite

Intra-articular injections of hyaluronic acid have been a mainstay of osteoarthritis treatment for decades. However, controversy surrounds the mechanism of action and efficacy of this therapy. As such, there has been recent interest in developing synthetic lubricants that lubricate cartilage. Recently, a synthetic 4 wt% polyacrylamide (pAAm) hydrogel was shown to effectively decrease lameness in horses.However, its mechanism of action and ability to lubricate cartilage is unknown. The goal of this study was to characterize the lubricating ability of this hydrogel and determine its efficacy for healthy and degraded cartilage. The study utilized previously established IL-1β-induced biochemical degradation and mechanical impact injury models to degrade cartilage. The lubricating ability of the hydrogel was then characterized using a custom-built tribometer using a glass counterface and friction was evaluated using the Stribeck framework for articular cartilage. pAAm hydrogel was shown to significantly lower the friction coefficient of cartilage explants from both degradation models (30%-40% reduction in friction relative to controls). A striking finding from this study was the aggregation of the pAAm hydrogel at the articulating surface. The surface aggregation was observed in the histological sections of explants from all treatment groups after tribological evaluation. Using the Stribeck framework, the hydrogel was mapped to higher Sommerfeld numbers and was characterized as a viscous lubricant predominantly in the minimum friction mode. In summary, this study revealed that pAAm hydrogel lubricates native and degraded cartilage explants effectively and may have an affinity for the articulating surface of the cartilage.

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 94%

Polyacrylamide hydrogel lubricates cartilage after biochemical degradation and mechanical injury

Vishwanath

McClure²,

Bonassar

2022

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Of the 20 trials identified, 25% were for bone, joint, or cartilage repair applications. These treatments implement hydrogels as tissue scaffolds, using either polyacrylamide or hydroxyethyl cellulose hydrogels to treat conditions that include arthrosis and osteoarthritis. Notably, these interventions generally do not aim to deliver drugs or therapeutic cells with hydrogels, with the exception of Argiform, a poly(acrylic acid) (PAA) gel formulated with antibacterial silver ions.…”

Section: Clinical Translation Of Biomedical Hydrogelsmentioning

confidence: 99%

Translational Applications of Hydrogels

et al. 2021

View full text Add to dashboard Cite

Advances in hydrogel technology have unlocked unique and valuable capabilities that are being applied to a diverse set of translational applications. Hydrogels perform functions relevant to a range of biomedical purposesthey can deliver drugs or cells, regenerate hard and soft tissues, adhere to wet tissues, prevent bleeding, provide contrast during imaging, protect tissues or organs during radiotherapy, and improve the biocompatibility of medical implants. These capabilities make hydrogels useful for many distinct and pressing diseases and medical conditions and even for less conventional areas such as environmental engineering. In this review, we cover the major capabilities of hydrogels, with a focus on the novel benefits of injectable hydrogels, and how they relate to translational applications in medicine and the environment. We pay close attention to how the development of contemporary hydrogels requires extensive interdisciplinary collaboration to accomplish highly specific and complex biological tasks that range from cancer immunotherapy to tissue engineering to vaccination. We complement our discussion of preclinical and clinical development of hydrogels with mechanical design considerations needed for scaling injectable hydrogel technologies for clinical application. We anticipate that readers will gain a more complete picture of the expansive possibilities for hydrogels to make practical and impactful differences across numerous fields and biomedical applications.

show abstract

“…Christensen et al (2016a) demonstrated integration of PAAG into the synovial layer in normal and osteoarthritic equine joints examined post-mortem up to 2 years post injection. Similar changes were demonstrated in normal rabbit knee joints up to 1 year post injection.…”

mentioning

confidence: 91%

In horses with osteoarthritis is treatment with intra-articular polyacrylamide gel more likely to reduce the severity of clinical signs associated with lameness when compared to treatment with intra-articular corticosteroid?

Bowkett‐Pritchard¹

2022

View full text Add to dashboard Cite

PICO question In horses with naturally occurring osteoarthritis, is treatment with intra-articular polyacrylamide gel more likely to reduce the severity of clinical signs associated with lameness when compared to treatment with intra-articular corticosteroid? Clinical bottom line Category of research question Treatment The number and type of study designs reviewed Twelve studies; four case series, three uncontrolled prospective studies, one non-blinded, non-randomised control trial, one non-blinded randomised control trial, two systematic reviews and one systematic review and meta-analysis Strength of evidence Weak Outcomes reported Studies examined: Clinical signs relating to lameness after use of corticosteroid or polyacrylamide gel to treat osteoarthritis; improvement in lameness and treatment success (including return to work in some papers) Conclusion It is not possible to recommend one treatment over the other given the absence of studies which provide direct comparison. This highlights the need for further controlled and comparative studies How to apply this evidence in practice The application of evidence into practice should take into account multiple factors, not limited to: individual clinical expertise, patient’s circumstances and owners’ values, country, location or clinic where you work, the individual case in front of you, the availability of therapies and resources. Knowledge Summaries are a resource to help reinforce or inform decision making. They do not override the responsibility or judgement of the practitioner to do what is best for the animal in their care.

show abstract

Synovial incorporation of polyacrylamide hydrogel after injection into normal and osteoarthritic animal joints

Abstract: Intra-articular injection of PAAG results in a stable, long-lasting sub-synovial layer of gel traversed with thin strands of connective tissue. Further studies to explore potential effects on synovial inflammation and pain are warranted.

Cited by 19 publications

References 11 publications

Polyacrylamide hydrogel lubricates cartilage after biochemical degradation and mechanical injury

Polyacrylamide hydrogel lubricates cartilage after biochemical degradation and mechanical injury

Translational Applications of Hydrogels

In horses with osteoarthritis is treatment with intra-articular polyacrylamide gel more likely to reduce the severity of clinical signs associated with lameness when compared to treatment with intra-articular corticosteroid?

Contact Info

Product

Resources

About