Synonymy of Pseudomonas avenae Manns 1905 and Pseudomonas alboprecipitans Rosen 1922

Schaad, N. W.; Kado, Clarence I.; Sumner, D. R.

doi:10.1099/00207713-25-2-133

Cited by 22 publications

(19 citation statements)

References 4 publications

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“…However, the latter bacterium is polarly multitichous, produces a yellow-green, nonfluorescent, diffusible pigment, utilizes glucose and sucrose, and does not infect watermelon. Based on the biochemical characteristics evaluated in t h s study, the watermelon bacterium also appeared to be similar to P. avenae (19). In our opinion, P. avenue reduces nitrate, utilizes glucose and mannose but not L-leucine, n-propanol, ethanol, or ethanolamine, and does not infect any of the cucurbits tested.…”

Section: Discussionmentioning

confidence: 89%

Pseudomonas pseudoalcaligenes subsp. citrulli subsp. nov.

Schaad

Sowell

Goth

et al. 1978

International Journal of Systematic Bacteriology

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119

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Ten nonfluorescent Pseudomonas strains isolated from water-soaked lesions on cotyledons of plants of five Citrullus lanatus (watermelon) plant introductions were characterized and compared phenotypically with 22 other pseudomonads. The strains were distinguished phenotypically from other known plant pathogenic pseudomonads. The watermelon bacterium was aerobic. Cells were rod-shaped, gram negative, and motile by means of a single polar flagellum. They were nonfluorescent and grew at 41°C but not at 4°C. Oxidase production and the 2-ketogluconate reaction were positive. The 10 strains utilized p-alanine, rA-leucine, D-serine, n-propanal, ethanol, ethanolamine, citrate, and fructose for growth. No growth occurred with sucrose or glucose. Their deoxyribonucleic acid base composition was 66 1 mol% guanine plus cytosine. The bacterium is phenotypically similar to P. pseudoalcaligenes but differs from it in being pathogenic to watermelon, Cucumis melo (cantaloupe), Cucumis sativus (cucumber), and Cucurbita pep0 (squash). The name P. pseudoalcaligenes subsp. citrulli is proposed for the new subspecies, of which strain C-42 (= ATCC 29625) is the type strain.For several years a bacterial disease of watermelons, characteAed by water-soaked lesions of cotyledons, has been observed on certain plant introductions at the Regional Plant Introduction Station, Experiment, Ga. A disease with similar symptoms on watermelon seedlings was reported to be caused by Pseudomonas lachrymans (9), but we could not isolate a pathogenic fluorescent pseudomonad. Our preliminary investigations showed that the causal bacterium was similar to the unidentified nonfluorescent pseudomonad isolated by Webb and Goth (22) from plants of watermelon plant introductions 174103 and 174104. The purpose of this investigation was to identify the causal bacterium isolated from diseased watermelon seedlings and to compare it with several other fluorescent and nonfluorescent pseudomonads, including the strain of P. lachrymans isolated from watermelon (9). Isolation and cultural properties. Seeds from different watermelon plant introductions were sown in trays (30 by 50 cm) of soil in a greenhouse with a day/night .ternpertwe of approximately 30/21 "C. Cotyledons and the first true leaves showing greasy, dark green lesions were collected, and the surfaces were disinfested in a freshly prepared 0.5% solution of sodium hypochlorite for 3 min. After rinsing for 2 min in sterile distilled water, the tissue from the lesions was removed and cut into smaller pieces in 0.1 ml of sterile distilled water, using a sterile scalpel. The suspension was streaked onto King medium B (KB; 11) and incubated for 5 days at 27°C (colonies of nonpathogenic pseudomonads were usually present after 2 days). Colonies of the watermelon bacterium were removed from the agar with a transfer loop and purified by streaking onto plates of KB. Stock cultures were maintained on KB slants. The media used for determining cultural characteristics were KB, yeast extract-dextrose-CaC03 (22), and Difco...

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Section: Discussionmentioning

confidence: 89%

Pseudomonas pseudoalcaligenes subsp. citrulli subsp. nov.

Schaad

Sowell

Goth

et al. 1978

International Journal of Systematic Bacteriology

Self Cite

119

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“…Other workers have recorded comparable values (72 mol% for P . avenue [24] and 66 mol% for P. pseudoalcaligenes subsp. citrulli [ l l , 251).…”

Section: Discussionmentioning

confidence: 99%

Numerical Analysis and Determinative Tests for Nonfluorescent Plant-Pathogenic Pseudomonas spp. and Genomic Analysis and Reclassification of Species Related to Pseudomonas avenae Manns 1909

Young²,

Triggs

1991

International Journal of Systematic Bacteriology

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We performed a numerical analysis of the results of biochemical and nutritional tests done with strains of nonfluorescent plant-pathogenic Pseudomonas spp. A total of 57 tests were used, and determinative tests which discriminated between taxa were identified. Pseudomonas andropogonis, P. caryophylli, P . corrugata, P . glumae, P . plantarii, and P . rubrisubalbicans were distinguished as distinct members of the genus Pseudomonas. Strains of P . Jicuserectae and P . meliae formed a single cluster having affinities with P . syringae. Strains of P . cissicola were allocated to Agrobacterium spp. Strains of P . avenae, P . cattleyae, P . pseudoalcaligenes subsp. citrulli, P . pseudoalcaligenes subsp. konjaci, and P . rubrilineans formed a single relatively homogeneous cluster, within which three subclusters were discerned. Strains presently identified as P . avenae and as P . rubrilineans, including the type strain of P . cattleyae, formed a single homogeneous subcluster. Strains of P . pseudoalcaligenes subsp. citrulli and P. pseudoalcaligenes subsp. konjaci formed two subclusters. Genomic probes prepared from DNAs of the type strains of P. avenae, P . cattleyae, P . pseudoalcaligenes subsp. citrulli, P . pseudoalcaligenes subsp. konjaci, and P . rubrilineans gave positive reactions with all of the strains of these species which were tested in colony hybridization studies, but not with strains of other nonfluorescent Pseudomonas spp. The G+C ratios of these type strains were all in the range from 67 to 71 mol%, and the levels of homology determined in DNA-DNA reassociation studies were all greater than 70%. P . avenae, P . cattleyae, P . pseudoalcaligenes subsp. citrulli, P . pseudoalcaligenes subsp. konjaci, and P . rubrilineans are considered to be members of a single species, P . avenae, with P . cattleyae and P . rubrilineans as junior synonyms; P . pseudoalcaligenes subsp. citrulli and P . pseudoalcaligenes subsp. konjaci are proposed as subspecies.The genus Pseudomonas Migula 1894, which at the present time is defined on the basis of phenetic data, is heterogeneous in its genomic relationships. An analysis of DNA-rRNA homologies has shown that the members of this genus fall into several distinct genetic groups which could be considered to be members of separate genera if the genetic data were supported by phenetic differences (21,22).Plant-pathogenic Pseudomonas spp. are distinguished according to whether they produce fluorescent pigments on iron-deficient media and produce poly-P-hydroxybutyrate inclusions (13,14 (22), are also members of this group (6).Organisms which are members of an "acidovorans" DNA-rRNA homology group (6) A numerical analysis of results of biochemical and nutritional tests performed with strains of nonfluorescent plantpathogenic Pseudomonas spp. (16) suggested that P. avenue, P. cattleyae, P. pseudoalcaligenes subsp. citrulli, and P. rubrilineans form a single homogeneous group, which was called the "avenae" cluster (16). The close relationship of the members of the avenae cluster wa...

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“…avenae and Acidovorax avenae subsp. cattleyae had the same chemotaxonomic characteristics and could be distinguished clearly from the genus Pseudomonas sensu strict0 (P. jluorescens rRNA branch [ll]), Burkholderia species, Comamonas species, and Hydrogenophaga species on the basis of phenotypic characteristics (10,17,28,35,38,44), ubiquinone systems, DNA base compositions, DNA-DNA homology data, and rRNA-DNA hybridization data (9,11,52,53) (Table 9). Minimal characteristics for differentiating the genera Burkholderia, Acidovorax, Comamonas, Hydrogenophaga, and Pseudomonas are shown in Table 9.…”

Section: + + + --+ + -+ + -+ + --+ + -mentioning

confidence: 99%