Syngeneic Polyclonal and Monoclonal Antiidiotypic Antibodies to Murine Anti-Human High Molecular Weight-Melanoma Associated Antigen and Anti-HLA Monoclonal Antibodies

Tsujisaki, Masayuki; Kusama, Mikihiro; Perosa, Federico; Sakaguchi, Kohsaku; Ferrone, Soldano

doi:10.1159/000415045

Cited by 3 publications

(2 citation statements)

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“…Previous studies for idiotype analysis using syngeneic murine anti-Id antibodies to anti-HLA (19,21,28), antimelanoma-associated antigen (29), and anti-CEA (30) MoAbs have demonstrated as follows: 1) polyclonal anti-Id antibodies (Ab2) which were induced with murine MoAb (Abl) in a syngeneic system inhibited the binding of MoAb (Abl) to antigen, suggesting that the polyclonal anti-Id antibodies included a population of anti-Id antibodies reactive with a combining site of MoAb; 2) idiotype mapping using anti-Id MoAbs showed that distinct idiotopes existed at both antigen com- noticeable that using this assay there is interference from the same anti-CEA antibodies as MoAb 5B3 in patient sera. From another point of view, anti-Id antibodies will be of use for measuring autoantibodies to antigens in sera.…”

Section: Discussionmentioning

confidence: 99%

New assay to detect shedding antigen with use of anti‐idiotypic monoclonal antibodies

Tsujisaki¹,

Imai²,

Tokuchi³

et al. 1991

Clinical Laboratory Analysis

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Anti-idiotypic (Anti-Id) monoclonal antibodies (MoAbs) against anti-carcinoembryonic antigen (CEA) monoclonal antibody 5B3 (IgG1) were prepared and characterized. Five anti-Id MoAbs recognized the private idiotype of MoAb 5B3. Idiotype mapping suggested that MoAb 5B3 had at least three distinct idiotopes at its antigen combining site. Utilizing the inhibition of idiotype-anti-idiotype interaction with use of anti-Id MoAb T4-305 or T4-212, an immunoassay to detect shedding CEA was established. The results of this inhibition assay correlated with those of double determinant immunoassay and inhibition percentages linearly increased in a CEA concentration-dependent manner. Since it requires only one epitope of a given antigen molecule, this assay could be widely used for detection of shedding antigens, including tumor-associated antigens.

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Section: Discussionmentioning

confidence: 99%

New assay to detect shedding antigen with use of anti‐idiotypic monoclonal antibodies

Tsujisaki¹,

Imai²,

Tokuchi³

et al. 1991

Clinical Laboratory Analysis

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show abstract

“…Anti-Id MoAbs (Ab2) were prepared against murine anti-CEA peptide MoAb P1-356 (Ab1) in a syngeneic system, using procedures described previously (10). Briefly, hybridomas were constructed with splenocytes from a BALB/c mouse primed with 100 mg of purified murine anti-CEA peptide MoAb P1-356 coupled to keyhole limpet hemocyanin and polymerized with glutaraldehyde in CFA (GIBCO Laboratories, Detroit, MI).…”

Section: Development Of Anti-idiotypic (Id) Moabmentioning

confidence: 99%

Preparation and characterization of anti‐anti‐idiotypic monoclonal antibody (“Ab1‐like Ab3”) in relation to carcinoembryonic antigen

Tsujisaki

Masuya

Okada

et al. 2002

Clinical Laboratory Analysis

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In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven anti-idiotypic monoclonal antibodies (anti-Id MoAbs) were generated from a BALB/c mouse immunized with anti-CEA MoAb P1-356, which recognized a synthetic peptide P1 of CEA, domain I. These MoAbs were divided into four groups. The anti-Id MoAbs specifically reacted with MoAb P1-356, but not with any MoAb, and inhibited the binding of MoAb P1-356 to CEA, indicating that all of these anti-Id MoAbs recognized private idiotopes at the combining sites of MoAb P1-356. Polyclonal anti-anti-idiotypic antiserum (Ab3) generated with anti-Id MoAbs M315 (Ab2), blocked the binding of MoAb P1-356 (Ab1) to CEA and reacted with CEA(Ag) and synthetic peptide P1. The analysis of serological assays suggested that it contains "Ab1-like Ab3." Therefore, we prepared anti-anti-Id MoAbs using anti-Id MoAb M315. Among 13 candidates, anti-anti-Id MoAb 11B2 was selected, because it competed with MoAb P1-356 (Ab1) binding to CEA. A direct binding assay using MoAb11B2 showed that it reacted with purified CEA and with CEA synthetic peptide P1. In addition, MoAb 11B2 (Ab3) reacted with both CEA-producing cultured cells and colonic cancerous tissues in immunostaining. These results indicate that anti-anti-Id MoAb 11B2 is "Ab1-like Ab3." Therefore, it is suggested that anti-Id MoAb M315 bears an "internal image" of the MoAb P1-356-defined epitope on CEA.

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New aspect of monoclonal antibody in clinical immunology: Immunological diagnosis and therapy in malignant diseases.

Yachi

Imai

1989

Jpn. J. Clin. Immunol.

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Syngeneic Polyclonal and Monoclonal Antiidiotypic Antibodies to Murine Anti-Human High Molecular Weight-Melanoma Associated Antigen and Anti-HLA Monoclonal Antibodies

Cited by 3 publications

References 0 publications

New assay to detect shedding antigen with use of anti‐idiotypic monoclonal antibodies

New assay to detect shedding antigen with use of anti‐idiotypic monoclonal antibodies

Preparation and characterization of anti‐anti‐idiotypic monoclonal antibody (“Ab1‐like Ab3”) in relation to carcinoembryonic antigen

New aspect of monoclonal antibody in clinical immunology: Immunological diagnosis and therapy in malignant diseases.

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