Synergy of ambroxol with vancomycin in elimination of catheter-related Staphylococcus epidermidis biofilm in vitro and in vivo

Zhang, Yunhui; Fu, Yakun; Yu, Jialin; Ai, Qing; Li, Junshuai; Peng, Ningning; Song, Sijie; He, Yu; Wang, Zhengli

doi:10.1016/j.jiac.2015.08.017

Cited by 27 publications

(24 citation statements)

References 32 publications

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“…During local immune activation it can dampen inflammation signals [27] and prevent pathogenic super infection by species such as Staphylococcus aureus [1; 2]. It can also act as a facultative pathogen forming biofilms on surgically implanted materials [5; 21; 49]. Recently a more distinct method of immune interaction via specific sub-dermal dendritic cells has been described [26] involving activation of the IL1 and IL17 signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Commensal bacterial modulation of the host immune response to ameliorate pain in a murine model of chronic prostatitis

Murphy

Schaeffer²,

Done³

et al. 2017

Pain

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The human commensal microflora plays an essential role in modulating the immune response to control homeostasis. Staphylococcus epidermidis, a commensal bacteria most commonly associated with the skin exerts such effects locally, modulating local immune responses during inflammation and preventing superinfection by pathogens such as Staphylococcus aureus. While the prostate is considered by many to be sterile, multiple investigations have shown that small numbers of gram-positive bacterial species such as S. epidermidis can be isolated from the expressed prostatic secretions (EPS) of both healthy and diseased men. Chronic pelvic pain syndrome (CPPS) is a complex syndrome with symptoms including pain and lower urinary tract dysfunction (LUTs). It has an unknown etiology and limited effective treatments but is associated with modulation of prostate immune responses. CPPS can be modeled using murine experimental prostatitis (EAP) where CD4+ve IL17A+ve T-cells have been shown to play a critical role in disease orchestration and development of pelvic tactile allodynia. Here we report that intra-urethral instillation of a specific S. epidermidis strain (designated NPI (non pain-inducing)), isolated from the EPS of a healthy human male, into EAP treated mice reduced the pelvic tactile allodynia responses and the increased CD4+ve IL17A+ve T-cell numbers associated with EAP. Furthermore, a cell wall constituent of NPI, lipotechoic acid (LTA), specifically recapitulates these effects and mediates increased expression of CTLA4-like ligands PDL1 and PDL2 on prostatic CD11b+ve antigen presenting cells. These results identify a new potential therapeutic role for commensal S. epidermidis NPI LTA in the treatment of prostatitis-associated pain.

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Section: Introductionmentioning

confidence: 99%

Commensal bacterial modulation of the host immune response to ameliorate pain in a murine model of chronic prostatitis

Murphy

Schaeffer²,

Done³

et al. 2017

Pain

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“…[27][28] More importantly, in vitro studies have demonstrated ABX's activity against bacterial infections. [21,29] These novel pharmacologic properties of ABX will greatly expand its application scope and amount, and its biological activity modulation studies are of high significance, which can be employed to deal with emerging problems related to its massive clinical administration. Therefore, in this work, the hostguest complexation between CB [7] and ABX was first studied, followed by the demonstration of CB [7]'s capability in modulating the antibacterial activity of ABX, in order to provide a simple, yet efficient method to regulate the biological activity of ABX, in this case, when used as an antimicrobial agent.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Modulation of Antibacterial Activity of Ambroxol by Cucurbit[7]uril

Kuok

Xià

et al. 2020

ChemPlusChem

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Supramolecular encapsulation by cucurbit [7]uril (CB[7]) was recently demonstrated to provide a simple and efficient method for antibacterial activity regulation of antibiotics. In this work, CB[7] was shown to form binary host-guest complex with ambroxol hydrochloride (ABX), a clinical mucokinetic and expectorant drug, which was reported to exhibit certain antibacterial activity. 1 H NMR titration and isothermal titration calorimetry experiment results suggested that the 4-hydroxyl cyclohexylamine group of ABX was included inside the CB[7] cavity, with a binding constant K a of (6.69 � 0.11) × 10 5 M À 1 in phosphate buffered saline (PBS) solution, thermodynamically driven by both enthalpy change (ΔH = À 12.2 kJ/mol) and entropy change (TΔS = 21.1 kJ/mol). More importantly, ABX's inhibitory activity (MIC 50 ) against bacillary strains towards Pseudomonas aeruginosa and Escherichia coli strains was decreased from (5.11 � 0.31) × 10 À 6 M À 1 and (2.63 � 0.34) × 10 À 5 M À 1 to zero upon encapsulation by CB [7], and was subsequently recovered to almost its original activity when a competitive guest, amantadine hydrochloride, for disassembling CB[7]-ABX complex, was added, suggesting that the antibacterial activity of ABX could be readily "turned off/on" upon its complexation and decomplexation with CB[7].[a] Dr.

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“…Premature infants are at a high risk of CoNS infections (6). drug for treatment of the MDR S. epidermidis (11). However, resistance to vancomycin has been frequently reported among the staphylococci (12,13).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Glycopeptide Susceptibility of Coagulase-Negative Staphylococci (CoNS) From a Tertiary Care Hospital in Gorgan, Northern Iran

Mehri

Jahanbakhsh

Shakeri

et al. 2016

Arch Pediatr Infect Dis

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Background: Coagulase-negative staphylococci (CoNS) are clinically important, especially in nosocomial and neonatal infections. The increasing emergence of glycopeptide-resistant CoNS has made these agents therapeutically challenging. Objectives: We aimed to investigate the susceptibility patterns of CoNS to teicoplanin and vancomycin in Gorgan, northern Iran. Materials and Methods: A total of 100 clinical samples were obtained from different wards of a hospital and screened for CoNS with standard microbiological and biochemical tests. Antibiogram testing was carried out for the detection of vancomycin-, teicoplanin-, and multidrug-resistant (MDR) species. The minimum inhibitory concentration (MIC) of vancomycin was determined using E-test strips. The presence of the vanA gene was investigated with PCR. Results: Only 1% of CoNS (Staphylococcus haominis) showed resistance to vancomycin and 15% of these were intermediate-resistant to teicoplanin on the disc diffusion agar test. In addition, all isolates (100%) were negative for vanA on PCR and sensitive with E-test

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Synergy of ambroxol with vancomycin in elimination of catheter-related Staphylococcus epidermidis biofilm in vitro and in vivo

Cited by 27 publications

References 32 publications

Commensal bacterial modulation of the host immune response to ameliorate pain in a murine model of chronic prostatitis

Commensal bacterial modulation of the host immune response to ameliorate pain in a murine model of chronic prostatitis

Supramolecular Modulation of Antibacterial Activity of Ambroxol by Cucurbit[7]uril

Investigation of Glycopeptide Susceptibility of Coagulase-Negative Staphylococci (CoNS) From a Tertiary Care Hospital in Gorgan, Northern Iran

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