Synergy Effect of Combining Fluorescence and Mid Infrared Fiber Spectroscopy for Kidney Tumor Diagnostics

Bogomolov, Andrey; Belikova, Valeria; Zabaryło, Urszula; Bibikova, Olga; Usenov, Iskander; Sakharova, Tatiana; Krause, Hans; Minet, Olaf; Feliksberger, Elena; Artyushenko, Viacheslav

doi:10.3390/s17112548

Cited by 17 publications

(20 citation statements)

References 39 publications

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“…During the in vivo studies, it was not impossible to certainly determine whether the exophytic and intracranial growth of tumor had occurred or not. Analysis of literature showed that usually registered fluorescence intensity under ultraviolet excitation has higher values in the intact area rather than in tumor [16][17][18][19][20] . Thus, it is possible to put forward a hypothesis that the first type spectra demonstrate the growth of tumor into the walls of the common bile duct.…”

Section: Resultsmentioning

confidence: 99%

Optical diagnostics of bile duct tissues state with tumor compression

Kandurova

Dremin²,

Zherebtsov

et al. 2019

Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine

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The paper presents the results of in vivo experimental measurements of the endogenous fluorescence and blood perfusion in common bile duct tissues in obstructive jaundice patients during minimally invasive surgical operations. Nowadays the type of interventions become more widespread for treating hepatopancreatobiliary pathologies. An effective feedback which can be established by one or more optical diagnostic channels integrated into the standard instruments for minimally invasive manipulations would be of particular value to practical surgeons. To collect reference data, an experimental study has been conducted using a fiber optical probe with fluorescence spectroscopy and laser Doppler flowmetry channels. For every measurement, the areas of interest were different areas of common bile duct obstructed by a malignant tumor. Obtained results demonstrated significant variety registered intensities of fluorescence spectra and values of blood perfusion. Tested fiber optical endoscopic system showed sensitivity to the tissue state in the investigated area, so the two modal approach seems to be promising for further research and implementation in clinical practice.

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Section: Resultsmentioning

confidence: 99%

Optical diagnostics of bile duct tissues state with tumor compression

Kandurova

Dremin²,

Zherebtsov

et al. 2019

Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine

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“…Accordingly, the medical application of MIR is fewer than for NIR. MIR optical fibers have been commercially available since 2016, whereas previously laboratory tools existed only [12][13][14]. In a study, breast cancer imaging of 15 patients was carried out using mid-and long wave infrared cameras.…”

Section: Introductionmentioning

confidence: 99%

Mid-Infrared Imaging Is Able to Characterize and Separate Cancer Cell Lines

Kontsek

Pesti

Björnstedt

et al. 2020

Pathol. Oncol. Res.

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Malignancies are still responsible for a large share of lethalities. Macroscopical evaluation of the surgical resection margins is uncertain. Big data based imaging approaches have emerged in the recent decade (mass spectrometry, two-photon microscopy, infrared and Raman spectroscopy). Indocianine green labelled MS is the most common approach, however, label free midinfrared imaging is more promising for future practical application. We aimed to identify and separate different transformed (A-375, HT-29) and non-transformed (CCD986SK) cell lines by a label-free infrared spectroscopy method. Our approach applied a novel setup for label-free mid-infrared range classification method. Transflection spectroscopy was used on aluminium coated glass slides. Both whole range spectra (4000-648 cm −1) and hypersensitive fingerprint regions (1800-648 cm −1) were tested on the imaged areas of cell lines fixed in ethanol. Non-cell spectra were possible to be excluded based on mean transmission values being above 90%. Feasibility of a mean transmission based spectra filtering method with principal component analysis and linear discriminant analysis was shown to separate cell lines representing different tissue types. Fingerprint region resulted the best separation of cell lines spectra with accuracy of 99.84% at 70-75 mean transmittance range. Our approach in vitro was able to separate unique cell lines representing different tissues of origin. Proper data handling and spectra processing are key steps to achieve the adaptation of this dye-free technique for intraoperative surgery. Further studies are urgently needed to test this novel, marker-free approach.

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“…In the last decade, it was demonstrated that a combination of fibre-optic approaches with the fusion of spectroscopic data from several methods increases the accuracy of sample differentiation by tens of percent [ 31 , 32 ]. Recently, authors demonstrated that a combination of a fibre-optic approach with the fusion of the spectroscopy data from several methods also increases the accuracy of tumour detection by 28% [ 33 ]. However, the majority of reported multispectral analyses present a combination of fluorescence and NIR spectroscopy methods [ 32 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…The latter enables the detection of different cancer types based on the qualitative and quantitative correlation of specific cancer biomarkers. MIR radiation penetrates the sample by up to 2 μm, which allows analysing individual cellular structures [ 33 ], while NIR radiation penetrates up to several mm into the tissue, allowing the evaluation of several tissue layers (e.g., epithelial cells, mucosa, muscular) [ 14 ]. We assume that this ability could vastly enhance the information collection process and become an essential part of the clinical translation of a new cancer diagnostic tool.…”

Section: Introductionmentioning

confidence: 99%

“…The authors’ recent work from this series was aimed at the development and testing of a NIR sensor for the diagnosis of kidney tumours based on light-emitting diodes (LEDs) [ 15 ]. Furthermore, the synergy effect of the concomitant MIR and fluorescence spectroscopy use for analysis of kidney tumours [ 33 ] or NIR and fluorescence spectroscopy for analysis of colon tumours was demonstrated [ 35 ]. In this study, we applied an equivalent approach.…”

Section: Introductionmentioning

confidence: 99%

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Synergy Effect of Combined Near and Mid-Infrared Fibre Spectroscopy for Diagnostics of Abdominal Cancer

Hocotz

Bibikova

Belikova

et al. 2020

Sensors

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Cancers of the abdominal cavity comprise one of the most prevalent forms of cancers, with the highest contribution from colon and rectal cancers (12% of the human population), followed by stomach cancers (4%). Surgery, as the preferred choice of treatment, includes the selection of adequate resection margins to avoid local recurrences due to minimal residual disease. The presence of functionally vital structures can complicate the choice of resection margins. Spectral analysis of tissue samples in combination with chemometric models constitutes a promising approach for more efficient and precise tumour margin identification. Additionally, this technique provides a real-time tumour identification approach not only for intraoperative application but also during endoscopic diagnosis of tumours in hollow organs. The combination of near-infrared and mid-infrared spectroscopy has advantages compared to individual methods for the clinical implementation of this technique as a diagnostic tool.

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Synergy Effect of Combining Fluorescence and Mid Infrared Fiber Spectroscopy for Kidney Tumor Diagnostics

Cited by 17 publications

References 39 publications

Optical diagnostics of bile duct tissues state with tumor compression

Optical diagnostics of bile duct tissues state with tumor compression

Mid-Infrared Imaging Is Able to Characterize and Separate Cancer Cell Lines

Synergy Effect of Combined Near and Mid-Infrared Fibre Spectroscopy for Diagnostics of Abdominal Cancer

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