Synergistically enhanced apoptotic and oxidative DNA damaging pathways in the rat brain with lead and/or aluminum metals toxicity: Expression pattern of genes OGG1 and P53

El‐Shetry, Eman S.; Mohamed, Amany Abdel-Rahman; Khater, Safaa I.; Metwally, Mohamed M.M.; Nassan, Mohamed A.; Shalaby, Shimaa I.; El-Mandrawy, Shefaa A.M.; Emran, Talha Bin; Abdel‐Ghany, Heba M.

doi:10.1016/j.jtemb.2021.126860

Cited by 16 publications

(6 citation statements)

References 67 publications

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“…In microscopic examination sections of brain from rats of toxic control groups of Pb and As showed gliosis, venous congestion, moderate neuronal degeneration and perivascular lymphoid aggregation with mild vacuolar changes. The histological changes in the brain in toxic groups coincide with the previous report of El-Shetry et al [11] observed lead caused neuronal toxicity in brain as lead treated rats showed brain oedema in the hippocampus area associated with focal gliosis in the cerebrum. The cerebellum showed vacuolization, while the medulla oblongata had neuronal degeneration and gliosis.…”

Section: Discussionsupporting

confidence: 89%

Effect of lemon peel extract gold nanoparticals on neurotoxicity induced by lead and arsenic in wistar rats

Tripura,

Hajare,

Kamdi

et al. 2024

Int. J. Adv. Biochem. Res.

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In Indian system of Ayurveda gold is used in the form of ash (Swarnabhasma) since 800AD mainly as a rejuvenator and its medical indications can be found in all ancient medical classics. Considering therapeutic potential of gold the current study was carried out to evaluate the antioxidant activities of Lemon peel extract gold Nanoparticals (LPGNP) supplement against lead acetate and sodium arseniteinduced nephrotoxicity in wistar rats. In this study, adults male rats were treated for 14 days with lead acetate @ 116.4 mg/kg, Sodium Arsenite (NaAsO2) @ 13.8mg/kg body weight p.o for 14days. Our study showed that supplementation with LPGNP brought improvement in oxidative stress-related parameters (SOD, CAT, Glutathione) and histopathology of brain showed corrective effect of LPGNP on Pb and As -induced brain neurotoxicity. Overall, LPGNP alleviated the toxic effects of these heavy metals on brain tissue, suggesting its role as a potential antioxidant and neuroprotective agent.

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Section: Discussionsupporting

confidence: 89%

Effect of lemon peel extract gold nanoparticals on neurotoxicity induced by lead and arsenic in wistar rats

Tripura,

Hajare,

Kamdi

et al. 2024

Int. J. Adv. Biochem. Res.

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“…Consistent with our results, previous in vivo and in vitro investigations clarified the involvement of the caspase-3 and Bcl-2 families in lead-mediated apoptosis [ 74 , 75 ]. Additionally, numerous studies have demonstrated that lead exposure triggers apoptosis through activation of tumor suppressor protein ( P53) and Bax [ 76 , 77 , 78 ]. The activation of these proteins exacerbates mitochondrial dysfunction [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Alhagi maurorum Ethanolic Extract Rescues Hepato-Neurotoxicity and Neurobehavioral Alterations Induced by Lead in Rats via Abrogating Oxidative Stress and the Caspase-3-Dependent Apoptotic Pathway

et al. 2022

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This work investigated the probable protective effect of an Alhagi maurorum ethanolic extract on the hepatotoxicity and neurotoxicity accompanied by neurobehavioral deficits caused by lead in rats. Rats in four groups were orally administered distilled water, ethanolic extract of A. maurorum (300 mg/kg BW daily), lead (100 mg/kg BW daily for 3 months), and lead + A. maurorum extract. The results demonstrated that lead exposure resulted in elevated locomotor activities and sensorimotor deficits associated with a decrease in brain dopamine levels. Moreover, lead exposure significantly increased liver function markers. In addition, the lead-treated rats exhibited extensive liver and brain histological changes and apoptosis. The lead treatment also triggered oxidative stress, as demonstrated by the increase in malondialdehyde (MDA) concentrations with a remarkable reduction in the activities of antioxidant enzymes, reduced glutathione (GSH) levels, and transcriptional mRNA levels of antioxidant genes in the liver and brain. Nevertheless, co-treatment with the A. maurorum extract significantly ameliorated the lead-induced toxic effects. These findings indicate that the A. maurorum extract has the ability to protect hepatic and brain tissues against lead exposure in rats through the attenuation of apoptosis and oxidative stress.

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“…Autophagy is the quality control system of cells wherein the damaged organelles are repaired and recycled [ 30 ]. It also assists the ubiquitin system in cellular recovery from toxic proteins [ 31 ]. Thus, autophagy promotes cell survival and positively regulates energy balance [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Total RNA extraction was performed as previously described [ 30 ]. For mRNA, 500 ng of that total RNA was reverse-transcribed as previously described [ 4 , 31 ]. In contrast, for miRNA, 10 ng were reverse-transcribed using the TaqMan™ Small RNA Assays (ThermoFisher Scientific, Waltham, MA, USA) following manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

β-Cell Autophagy Pathway and Endoplasmic Reticulum Stress Regulating-Role of Liposomal Curcumin in Experimental Diabetes Mellitus: A Molecular and Morphometric Study

et al. 2022

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Background: Autophagy can confer protection to pancreatic β-cells from the harmful effects of metabolic stress by delaying apoptosis. Curcumin (CUR) alleviates oxidative and endoplasmic reticulum (ER) stress, activates autophagy, reduces inflammation, and decreases β-cell damage in type I diabetes. Liposomal CUR (LPs-CUR) has a higher therapeutic value and better pharmacokinetics than CUR. Objectives: We determined LPs-CUR’s ability to alleviate stress, reduce β-cell damage and unraveled the mechanism underlying its protective effect using a streptozotocin (STZ)-induced type I diabetic rat model. Methods: Sprague–Dawley rats were grouped into vehicle control, STZ-diabetic (STZ 65 mg/kg), STZ-diabetic-3-MA (3-methyladenine [3-MA] 10 mg/kg b.wt), STZ. diabetic-LPs-CUR (LPs-CUR 10 mg/kg b.wt), and STZ diabetic-LPs-CUR-3-MA (LPs-CUR 10 mg/kg b.wt; 3-MA 10 mg/kg b.wt). Results: LPs-CUR significantly reduced blood glucose, oxidative stress, and cellular inflammation in the pancreatic tissue (p < 0.001). ER stress-dependent genes included ATF-6, eIF-2, CHOP, JNK, BiP, and XBP LPs-CUR significantly suppressed fold changes, while it upregulated the autophagic markers Beclin-1 and LC3-II. Conclusions: LP-CUR ameliorates β-cell damage by targeting the autophagy pathway with the regulatory miRNAs miR-137 and miR-29b, which functionally abrogates ER stress in β-cells. This study presents a new therapeutic target for managing type I diabetes using miR-137 and miR-29b.

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Synergistically enhanced apoptotic and oxidative DNA damaging pathways in the rat brain with lead and/or aluminum metals toxicity: Expression pattern of genes OGG1 and P53

Cited by 16 publications

References 67 publications

Effect of lemon peel extract gold nanoparticals on neurotoxicity induced by lead and arsenic in wistar rats

Effect of lemon peel extract gold nanoparticals on neurotoxicity induced by lead and arsenic in wistar rats

Alhagi maurorum Ethanolic Extract Rescues Hepato-Neurotoxicity and Neurobehavioral Alterations Induced by Lead in Rats via Abrogating Oxidative Stress and the Caspase-3-Dependent Apoptotic Pathway

β-Cell Autophagy Pathway and Endoplasmic Reticulum Stress Regulating-Role of Liposomal Curcumin in Experimental Diabetes Mellitus: A Molecular and Morphometric Study

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