Synergistic use of electroosmotic flow and magnetic forces for nucleic acid extraction

Abstract

NA extraction and purification utilitzing a microfluidic chip with applied electric field to induce electroosmotic flow opposite the magnetic NA-bound bead mix.

Integrated magneto–electrophoresis microfluidic chip purification on library preparation device for preimplantation genetic testing for aneuploidy detection

“…This may be beneficial for purifying a sample of non-charged particles depending on the application. Electroosmotic flow and magnetic bead movement has been explored previously 35 but not for NGS library preparation to date. Overall, the preliminary data for this microfluidic chip and library preparation device presented in the current study has demonstrated that it can deliver high quality, reliable NGS library preparations and an accurate PGT-A result in an efficient amount of time with minimal user interaction.…”

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

“…By controlling the position of the magnetic field, magnetic beads carrying DNA can be made to pass through each flow sequentially. This work simplifies the procedure because the magnetic beads could pass through the main channel and provide effective separation within 3 s. Deraney et al (2020) incorporated use of an electric field into an existing magnetic separation chip; DNA extraction yield was increased by 15% thanks to the electroosmotic flow. Finally, bio-specific molecular interactions, such as antigen–antibody interaction, can be used to specifically separate target DNA.…”

Nucleic acid extraction: Fundamentals of sample preparation methodologies, current advancements, and future endeavors

“…[63] Deraney and co-workers devised a synergistic method that integrated magnetic and electroosmotic forces in their microfluidic system to transport DNA-enriched silica-based magnetic beads within the chip. [64] Utilizing electroosmotic flow improved the DNA extraction yield 15% compared to extractions without electroosmotic flow while the carryover volume volume of unwanted contaminants was minimized to only 0.22%. Zhang et al used a magnetic field to adhere silica-based magnetic beads to a coaxial channel.…”