Synergistic strategies of predominant toxins in snake venoms

Xiong, Shengwei; Huang, Chunhong

doi:10.1016/j.toxlet.2018.02.004

Cited by 57 publications

(59 citation statements)

References 186 publications

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“…Some are procoagulant and others are anticoagulant [61]. Thus, synergistic effect [14,62] and a balance of actions produce the final venom activity. Possibly, specific platelet aggregation assays could be used to evaluate the activities of disintegrins of the different genders.…”

Section: Discussionmentioning

confidence: 99%

Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?

Simizo

Kitano

Sant’Anna

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Bothrops atrox is known to be the pit viper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including northern South America, the east of Andes and the Amazon basin. Possibly, due to its wide distribution and generalist feeding, intraspecific venom variation was reported by previous proteomics studies. Sex-based and ontogenetic variations on venom compositions of Bothrops snakes were also subject of proteomic and peptidomic analysis. However, the venom peptidome of B. atrox remains unknown. Methods: We conducted a mass spectrometry-based analysis of the venom peptides of individual male and female specimens combining bottom-up and top-down approaches. Results: We identified in B. atrox a total of 105 native peptides in the mass range of 0.4 to 13.9 kDa. Quantitative analysis showed that phospholipase A 2 and bradykinin potentiating peptides were the most abundant peptide families in both genders, whereas disintegrin levels were significantly increased in the venoms of females. Known peptides processed at non-canonical sites and new peptides as the Ba1a, which contains the SVMP BATXSVMPII1 catalytic site, were also revealed in this work. Conclusion: The venom peptidomes of male and female specimens of B. atrox were analyzed by mass spectrometry-based approaches in this work. The study points to differences in disintegrin levels in the venoms of females that may result in distinct pathophysiology of envenomation. Further research is required to explore the potential biological implications of this finding.

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Section: Discussionmentioning

confidence: 99%

Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?

Simizo

Kitano

Sant’Anna

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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“…In addition to crotoxin, other PLA 2 s are present in the C. durissus venom composition. Besides neurotoxicity, PLA 2 s in general also cause cardiotoxicity, myotoxicity, hemorrhage, as well as edema, convulsions, hyperalgesia, inflammation, hypotension, inhibition of platelet aggregation, anticoagulation and hemolysis [63]. In the crotamine-positive pool, the relative abundance of CTX and PLA 2 is lower than in the crotaminenegative pool, with 28.71% and 45.94% of CTX, and 2.04% and 5.97% of PLA 2 , respectively.…”

Section: Differences Between Crotamine-positive and Crotamine-negativmentioning

confidence: 99%

“…Identified as the second most abundant component of C. durissus venom, snake venom serine proteases (SVSP) affect the victim hemostasis, disturbing mainly the coagulation system [63]. The crotamine-positive venom pool has slightly higher abundance of SVSP than crotamine-negative pool (25.03% and 22.85%, respectively).…”

Section: Differences Between Crotamine-positive and Crotamine-negativmentioning

confidence: 99%

Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature

Tasima

Serino-Silva

Hatakeyama

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or-positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups. Methods: The presence of crotamine was analyzed by SDS-PAGE, western blotting and ELISA, whereas comparison between the two types of venoms was carried out through HPLC, mass spectrometry analysis as well as assessment of antivenom lethality and efficacy. Results: The results showed a variation in the presence of crotamine among the subspecies and the geographic origin of snakes from nature, but not in captive snakes. Regarding differences between crotamine-positive and-negative venoms, some exclusive proteins are found in each pool and the crotamine-negative pool presented more phospholipase A 2 than crotamine-positive pool. This variation could affect the time to death, but the lethal and effective dose were not affected. Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom.

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“…Las toxinas de tres dedos 3FTx, son estructuras polipeptídicas no enzimáticas, conformadas por 60 a 74 residuos de aminoácidos y constituyen uno de los principales componentes de los venenos de Elápidos. (57).…”

Section: • Toxinas 3ftxunclassified

“…Estructuralmente tienen tres bucles conformados por láminas beta que se extienden desde un pequeño núcleo hidrofóbico globular que está unido por cuatro puentes disulfuro conservados, por lo cual son identificadas con este nombre (toxinas de tres dedos) debido a la forma tridimensional que adoptan (57,58).…”

Section: • Toxinas 3ftxunclassified

Actividad neurotóxica del veneno de serpientes del género Micrurus y métodos para su análisis. Revisión de la literatura

Bolívar-Barbosa

Rodríguez-Vargas

2020

Rev. Fac. Med.

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Este artículo fue aprobado para publicación en el v68n3 de la Revista de la Facultad de Medicina teniendo en cuenta los conceptos de los pares evaluadores y los cambios realizados por los autores según estos conceptos. Por lo tanto, se publica la versión preliminar del artículo para su consulta y citación provisional, pero debe aclararse que esta puede diferir del documento final, ya que no ha completado las etapas finales del proceso editorial (corrección de estilo, traducción y diagramación) y solo los títulos, datos de autores, palabras clave y resúmenes corresponden a la versión final del artículo.Esta versión puede consultarse, descargarse y citarse según se indique a continuación, pero debe recordarse que el documento final (PDF, HTML y XML) puede ser diferente. Actividad neurotóxica del veneno de serpientes del género Micrurus y métodos para su análisis. Revisión de la literatura]. Rev. Fac. Med. 2020;68(3): In press -2020. English. doi: Neurotoxical activity of Micrurus snakes venom and methods for its analysis. A literature review. Rev. Fac. Med. 2020;68(3): In press -2020. English. doi: http://dx.doi.org/10.15446/revfacmed.v68n3.75992. Revista de la Facultad de MedicinaIn press publication Rev. Fac. Med. P u b l i c a c i ó n a n t i c i p a d a . R e v . F a c . M e d . Tipo de artículo: Artículo de revisión DOI: http://dx.doi.org/10.15446/revfacmed.v68n3.75992 Neurotoxical activity of Micrurus snakes venom and methods for its analysis. A literature review Actividad neurotóxica del veneno de serpientes del género Micrurus y métodos para su análisis. Revisión de la literatura Serpientes Micrurus: neurotoxicidad del veneno Abstract Introduction: Snakes of the genus Micrurus have fossorial habits, passive temperament and scarce production of powerful venom with neurotoxic characteristics that block the synaptic transmission at the neuromuscular junction. Objective: To present an overview of the neurotoxicity of the Micrurus snake venom, and its functional characterization by ex vivo analysis methods. Materials and methods: A literature review was conducted in MedLine and ScienceDirect using specific terms and their combinations. Search strategy: type of studies: articles on the neurotoxicity of Micrurus snake venom and techniques to determine its neurotoxic activity by in vitro, in vivo and ex vivo models; publication period: articles published until June 2018; publication language: English and Spanish.Results: Of 88 studies identified in the initial search, 28 were excluded because they did not meet the inclusion criteria (based on reading titles and abstracts). 8 additional articles (books and reports) were included, since, according to the authors' opinion, they complemented the information reported by the selected studies. The studies included in the review (n=68) were original research papers (n=44), review articles (n=16), and book chapters, reports, guides and online consultations (n=8). Conclusions:Studies performed using ex vivo muscle and nerve preparations to evaluate the effect of neurotoxins ...

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Synergistic strategies of predominant toxins in snake venoms

Cited by 57 publications

References 186 publications

Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?

Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?

Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature

Actividad neurotóxica del veneno de serpientes del género Micrurus y métodos para su análisis. Revisión de la literatura

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