Synergistic roles of LAX1 and FZP in the development of rice sterile lemma

Wang, Yidong; Wei, S. H.; He, Yubing; Yan, Lang; Wang, Rongchen; Zhao, Yunde

doi:10.1016/j.cj.2019.06.006

Cited by 5 publications

(7 citation statements)

References 32 publications

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“…Clustered regularly interspaced short palindromic repeats (CRISPR)‐associated protein 9 (Cas9) is an efficient tool used in rice and other crop breeding for desired traits (Zheng et al ., 2016; Lee et al ., 2019; Li et al ., 2019; Ma et al ., 2019; Wang et al ., 2019; Wang et al ., 2020). OsDGD2β is a digalactosyldiacylglycerol synthase in anther.…”

Section: Introductionmentioning

confidence: 99%

Creation of male‐sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two‐line system for the hybrid production of rice (Oryza sativa L.)

Pak

Wang

Kim³

et al. 2020

Plant Biotechnology Journal

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Male sterility is widely used in the production of hybrid seeds in rice, but the use of genic male sterility is limited because of the high labour cost for maintaining male‐sterile lines. Previous studies using T‐DNA insertional mutagenesis demonstrated that disrupting the expression of oxophytodienoic acid reductase 3 (OPR3), which is involved in the jasmonate biosynthesis pathway, results in a kind of male sterility that can be restored to fertility by exogenous jasmonate in Arabidopsis. Here, we created male‐sterile mutations by editing the second and fourth exons of OsOPR7 in rice through clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated system 9. The induced mutagenesis at these exons resulted in 31.8% and 23.9% male‐sterile plants in the T0 generation, respectively. We screened male‐sterile lines that can be restored to fertility by exogenous methyl jasmonate in the T0, T1 and T2 rice populations and characterized the anther and agronomic traits of the transgenic plants. Results showed the successful generation of male‐sterile lines through the silencing of OsOPR7, the orthologous gene of Arabidopsis OPR3, in a field crop, paving the way for the establishment of a two‐line system for rice hybrid production. The system consists of a male‐sterile line that can be maintained by spraying methyl jasmonate and a restoring line that confers pollen.

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Section: Introductionmentioning

confidence: 99%

Creation of male‐sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two‐line system for the hybrid production of rice (Oryza sativa L.)

Pak

Wang

Kim³

et al. 2020

Plant Biotechnology Journal

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“…FZP encodes an AP2 family transcription factor and plays essential roles in rice panicle development [ 18 ]. To date, 18 fzp mutants have been reported in rice [ 18 , 19 , 21 , 29 , 30 , 31 ], with most due to mutation in the coding or 5’UTR region of FZP , causing multiple phenotypes [ 31 , 32 ]. Weak fzp mutants like sgdp7 or qSrn7 have smaller but more spikelets per panicle [ 19 , 30 ]; fzp-12 and fzp-13 display defects in the sterile lemma identity [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression of MOTHER OF FT AND TFL1 ( OsMFT1 ) can inhibit the transcription of FZP , thereby producing more secondary branches [ 20 ]. LAX1 encodes a helix-loop-helix (bHLH) transcription factor, determines the initiation and maintenance of axillary meristem, and synergistically regulates sterile lemma development in rice with FZP [ 21 ]. The lax1 mutant exhibits reduced tillers and lateral spikelets [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Panicle Apical Abortion 7 Regulates Panicle Development in Rice (Oryza sativa L.)

Dai

Zhang

et al. 2022

IJMS

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The number of grains per panicle significantly contributes to rice yield, but the regulatory mechanism remains largely unknown. Here, we reported a loss-of-function mutant, panicle apical abortion 7 (paa7), which exhibited panicle abortion and degeneration of spikelets on the apical panicles during the late stage of young panicle development in rice. High accumulations of H2O2 in paa7 caused programmed cell death (PCD) accompanied by nuclear DNA fragmentation in the apical spikelets. Map-based cloning revealed that the 3 bp “AGC” insertion and 4 bp “TCTC” deletion mutation of paa7 were located in the 3′-UTR regions of LOC_Os07g47330, which was confirmed through complementary assays and overexpressed lines. Interestingly, LOC_Os07g47330 is known as FRIZZY PANICLE (FZP). Thus, PAA7 could be a novel allele of FZP. Moreover, the severe damage for panicle phenotype in paa7/lax2 double mutant indicated that PAA7 could crosstalk with Lax Panicle 2 (LAX2). These findings suggest that PAA7 regulates the development of apical spikelets and interacts with LAX2 to regulate panicle development in rice.

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“…FZP suppresses panicle branching and positively regulates floral meristem identity (Komatsu et al., 2003). Hitherto, at least 15 fzp allelic mutants have been identified and most mutants show abnormal branch patterning with high‐order branches and no formation of spikelets (Bai et al., 2016; Ikeda‐Kawakatsu et al., 2012; Kato and Horibata, 2012; Komatsu et al., 2003; Ren et al., 2018; Wang et al., 2019; Yi et al., 2005; Zhu et al., 2003). An 18‐bp copy number variant (CNV) insertion at ~5.3 kbp upstream of FZP in Chuan 7 and several aus varieties inhibits FZP expression, resulting in an increase in the number of secondary branches and rice production (Bai et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, FZP negatively regulates the expression of RFL / APO2 (Bai et al., 2016) and several genes controlling grain size, but positively regulates OsMADS ‐box genes that are involved in specifying the identity of floral organs (Bai et al., 2016; Ren et al., 2018). LAX1 and FZP synergistically control sterile lemma development (Wang et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel mutant allele, fzp‐15, involved in panicle branch pattern of rice (Oryza sativa)

et al. 2021

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FRIZZY PANICLE (FZP) is involved in the development of panicle branching in rice. Herein, a novel mutant fzp‐15, demonstrating the sequential rounds of branching and severe defects in SM (spikelet meristem) formation, was identified. Map‐based cloning revealed that the abnormal panicle architecture of fzp‐15 resulted from an amino acid substitution (Glu (E) to Ala (A)) in the N‐terminal ERF domain of rice FZP protein. To generate new advantageous FZP alleles increasing grain numbers, CRISPR/Cas9 multiplex editing system was used to target the 5’ untranslation region (UTR) of FZP in 'Nipponbare' (NIP). The 85‐bp (−156 to ‐72 bp) or 149‐bp (−157 to ‐9 bp) deletion in the upstream region of FZP can significantly increase grain numbers without a decrease in grain size, while the 38‐bp deletion (−44 to −7 bp) in the upstream region of FZP has little effect in changing grain numbers. These results indicated that the 113‐bp fragment (−157 to −45 bp) upstream of FZP is important to its function and deletion of this region can be used to increase grain numbers in rice.

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Synergistic roles of LAX1 and FZP in the development of rice sterile lemma

Cited by 5 publications

References 32 publications

Creation of male‐sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two‐line system for the hybrid production of rice (Oryza sativa L.)

Creation of male‐sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two‐line system for the hybrid production of rice (Oryza sativa L.)

Panicle Apical Abortion 7 Regulates Panicle Development in Rice (Oryza sativa L.)

Identification of a novel mutant allele, fzp‐15, involved in panicle branch pattern of rice (Oryza sativa)

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