Synergistic role of β-catenin1 and 2 in ovarian differentiation and maintenance of female pathway in Nile tilapia

Wu, Limin; Wu, Fengrui; Xie, Lang; Wang, Deshou; Zhou, Lei

doi:10.1016/j.mce.2016.03.002

Cited by 24 publications

(30 citation statements)

References 42 publications

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“…The real‐time PCR data showed that β ‐catenin 1 transcripts were ubiquitously distributed in various tissues in O. niloticus , with higher level in liver, gonads and kidney (Wu et al., ). Up to now, there has been no report showing the mRNA expression and cellular localization of the β‐catenin in other fish.…”

Section: Discussionmentioning

confidence: 99%

“…The level of β ‐catenin 1 mRNA also increased in early stage of ovary development period, but decreased in fully mature period and increased in ovary recession period again. In O. niloticus , β ‐catenin 1 expression undergoes a stepwise increase in the ovaries during 20, 60 and 180 days after hatching development (Wu et al., ). The increasing level of β ‐catenin 1 mRNA in early ovary development period of C. carpio and O. niloticus, indicating that β ‐catenin 1 plays an important role during fish ovarian differentiation and development (Kobayashi & Nagahama, ; Wu et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…In O. niloticus , β ‐catenin 1 expression undergoes a stepwise increase in the ovaries during 20, 60 and 180 days after hatching development (Wu et al., ). The increasing level of β ‐catenin 1 mRNA in early ovary development period of C. carpio and O. niloticus, indicating that β ‐catenin 1 plays an important role during fish ovarian differentiation and development (Kobayashi & Nagahama, ; Wu et al., ). In this study, the fish ovary degenerated and proceeded to next development cycle at recession period.…”

Section: Discussionmentioning

confidence: 99%

“…β ‐catenin 1 and β ‐catenin 2 were identified in zebrafish and they played essential roles in the formation of dorsal axial structures and the neurectoderm (Bellipanni et al., ; Zhang, Zhang, Lin & Meng, ). Synergistic role of β ‐catenin 1 and β ‐catenin 2 in O. niloticus suggested that β ‐catenins are important members and play synergistic roles in the canonical Wnt signal pathway and Foxl2‐leading oestrogen pathway might be involved in ovarian differentiation (Wu, Wu, Xie, Wang & Zhou, ). However, there has been no more information about the roles of β ‐catenin in fish gonad development.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

et al. 2017

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β‐catenin gene is a pivotal gene for gonad development and maintenance of ovarian function in mammals. However, little is known about its expression and function in gonad development of fish. In this study, a complete cDNA (3342 bp) sequence of β‐catenin 1 was cloned from the common carp, Cyprinus carpio, by RACE PCR, which encodes a 780‐amino‐acid protein. Quantitative real‐time PCR demonstrated that β‐catenin 1 mRNA expressions were high in the testis and ovary tissue and the expression increased as the testes developed and the early stage ovaries developed. Western blot results revealed a single immunoreactive band with an estimated molecular weight of 90 kDa in testes. Immunohistochemistry analysis revealed that the β‐catenin 1 protein was concentrated mainly in the cytoplasm of early development stage of oocyte cells and in the cytomembrane of developing and mature sperm cells. 17β‐Ethinylestradiol injecting intraperitoneally into the fish decreased the relative β‐catenin 1 mRNA expression level except 1 μg/g 72 hr and 5 μg/g 48 hr of treatments in the ovary by real‐time PCR. These results suggest, for the first time, that β‐catenin 1 is an essential protein in gonad development and might be involved in ovarian early development of C. carpio.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

et al. 2017

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“…, ; Vizziano‐Cantonnet et al ., ; Wu et al . , ; Yang et al ., ), two stress related response genes ( crf and gcr1 ; Castañeda‐Cortés et al ., ; Cowan et al ., ; Hattori et al ., ) and four ROS antagonist effector genes ( cat , sod2 , gsr , ucp2 ; Giannetto et al . , ; Toda & Diano, ; Vozza et al ., ; Yuan et al ., ), using IDT primer quest software (http://www.idtdna.com) based on a de novo transcriptome database (BioProject: PRJNA401428) of C. estor .…”

Section: Methodsmentioning

confidence: 99%

Experimental evidence of masculinization by continuous illumination in a temperature sex determination teleost (Atherinopsidae) model: is oxidative stress involved?

Corona-Herrera

Arranz

Martı́nez-Palacios

et al. 2018

Journal of Fish Biology

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The present study evaluates the influence of continuous light on phenotypic sex ratios in Chirostoma estor, a temperature sex determination animal model. Relative gene expression levels of 5 day old larvae were performed on two early gonad differentiation genes (sox9 and foxl2), two stress axis activation genes (gcr1 and crf) and four reactive oxygen species (ROS) antagonist effector genes (sod2, ucp2, gsr and cat). Two light treatments were applied from fertilization; control (12L:12D) simulated natural photoperiod and a continuous illumination photoperiod. By the end of the trial (12 weeks after hatching), differentiated and normal gonads were clearly identifiable in both treatments by histological observations. Regarding sex ratio, 73% of phenotypic males were found in continuous illumination compared with 40% in controls. Consistently, the sox9 gene (involved in early testis differentiation) showed an over expression in 64% of the individual larvae analysed compared with foxl2 (ovarian differentiation) suggesting a masculinization tendency in continuous illumination. On the other hand, only 36% of individuals showed the same tendency in the control treatment consistent with phenotypic sex ratios found under normal culture conditions. Relative gene expression results did not show significant difference in sod2, ucp2 and gcr1 levels, but cat, gsr and crf showed significantly higher expression levels in the continuous illumination treatment suggesting that both, the stress axis and ROS response mechanisms were activated at this time. This study suggests, a link between continuous light, oxidative stress and environmental sex determination in vertebrates. However, further research is necessary to describe this possible upstream mechanism that may drive some aspects of sexual plasticity in vertebrates.

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Gene Editing Technology for Fish Health Management

Gupta

2021

Biotechnological Advances in Aquaculture Health Management

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Synergistic role of β-catenin1 and 2 in ovarian differentiation and maintenance of female pathway in Nile tilapia

Cited by 24 publications

References 42 publications

Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

Experimental evidence of masculinization by continuous illumination in a temperature sex determination teleost (Atherinopsidae) model: is oxidative stress involved?

Gene Editing Technology for Fish Health Management

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