Synergistic Regulation of Microglia Gene Expression by Natural Molecules in Herbal Medicine

Qiburi, Qiburi; Temuqile, Temuqile; Baigude, Huricha

doi:10.1155/2021/9920364

Cited by 4 publications

(4 citation statements)

References 39 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been confirmed that EW improves neurobehaviors in rodents with middle artery occlusion-reperfusion injury and has specific preventive and therapeutic effects in treating stroke [15][16][17]. EW can also markedly decrease the cytokine expressions for inflammation, such as TNF-α, IL-6, and IL-1β in the brain, decrease apoptosis, and improve nervous system symptoms related to inflammation [15,18,19]. The EW basic formulation consists of 10 drugs for the treatment of nervous system diseases, selected from EW formulation based on Mongolian medical theory.…”

Section: Introductionmentioning

confidence: 87%

“…Currently, the administration of EW in the therapeutic application of neuronal dysfunction is attracting increasing attention and many related studies have been conducted in China. It has been confirmed that EW improves neurobehaviors in rodents with middle artery occlusion-reperfusion injury and has specific preventive and therapeutic effects in treating stroke [15][16][17]. EW can also markedly decrease the cytokine expressions for inflammation, such as TNF-α, IL-6, and IL-1β in the brain, decrease apoptosis, and improve nervous system symptoms related to inflammation [15,18,19].…”

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Study on the Mechanism of Eerdun Wurile’s Effects on Post-operative Cognitive Dysfunction by the TLR4/NF-κB Pathway

Qiao,

Li,

et al. 2023

Mol Neurobiol

View full text Add to dashboard Cite

The object of our work was to observe whether the Mongolian medicine Eerdun Wurile (EW) improve postoperative cognitive dysfunction (POCD) by affecting the TLR4/NF-κB. Mice (6–8-week-old male C57BL/6 J) were selected to establish an animal model of POCD by combining intracerebroventricular injection of lipopolysaccharide and nephrectomy; EW formulation and EW basic formulation were administered intra-gastrically for 7 consecutive days. The cognitive performance was assessed by Morris water maze test. H&E staining was examined to detect alterations in hippocampal tissue. Immunohistochemical staining was performed to evaluate MyD88, NF-κB, TLR4, iNOS, and IBA-1 expressions; Western blotting and RT-qPCR were performed to evaluate MyD88, NF-κB, and TLR4. The expressions of IL-6, IL-1β, and TNF-α were evaluated by ELISA. Intracerebroventricular injection of lipopolysaccharide combined with nephrectomy induced cognitive dysfunction in mice, stimulated TLR4/NF-κB and microglia, and promoted the secretion of murine TNF-α, IL-1β, and IL-6. EW formulation and EW basic formulation treatment are able to suppress the TLR4/NF-κB pathway activation and microglia, and the serum cytokine secretions related to proinflammation, and restore the cognitive performance. EW formulation and EW basic formulation can improve POCD in mice, and TLR4/NF-κB pathway seems to be one of the important mechanisms in EW’s improvement of POCD.

show abstract

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 92%

Study on the Mechanism of Eerdun Wurile’s Effects on Post-operative Cognitive Dysfunction by the TLR4/NF-κB Pathway

Qiao,

Li,

et al. 2023

Mol Neurobiol

View full text Add to dashboard Cite

show abstract

“…Luteolin is a flavonoid compound that protects mouse neurons and alleviates depressionlike behavior in CUMS mice via the Sirt1/NF-κB/NLRP3 signaling Frontiers in Pharmacology frontiersin.org pathway (Xie et al, 2023). Dehydrodiisoeugenol, which play antiinflammatory and antioxidant roles via regulation of canonical pathways such as nuclear factor kappa B (NF-κB) in microglia and microphages (Qiburi et al, 2021). Regeneration of nerves is a complex process involving multiple pathways.…”

Section: Effect Of Ecs On Nf-κb Signaling In the Hippocampi Of Mice W...mentioning

confidence: 99%

Investigation of anti-depression effects and potential mechanisms of the ethyl acetate extract of Cynomorium songaricum Rupr. through the integration of in vivo experiments, LC-MS/MS chemical analysis, and a systems biology approach

Zhang,

Li,

Chen

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

Background:Cynomorium songaricum Rupr. has long been used as an anti-inflammatory, antidepressant, and anti-aging agent in traditional Chinese medicine in Asia. Its ethyl acetate extract (ECS) has been identified as the main antioxidant component with neuroprotective and estrogen-like effects. However, the potential of ECS in treating depression has not been explored yet.Methods: We identified the primary metabolites in ECS in this study using liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS). Network analysis was used to find the potential targets and pathways associated with the anti-neuroinflammatory depression action of the ECS. In addition, we established a corticosterone (CORT)-induced depression mouse model to assess ECS’s antidepressant effects by monitoring various behavioral changes (e.g., sucrose preference, forced swimming, tail suspension, and open field tests) and biochemical indices of the hippocampus, and validating the network analysis results. Significant pathways underwent verification through western blotting based on network analysis prediction.Results: Our study demonstrates that ECS possesses significant antidepressant activity. The LC-MS/MS analysis of ECS identified 30 main metabolites, including phloridzin, phlorizin, ursolic acid, and naringenin, as well as other flavonoids, terpenoids, and phenolic acids. These metabolites were found to be associated with 64 candidate target proteins related to neuroinflammatory depression from the database, and ten hub proteins were identified through filtration: CXCL8, ICAM1, NOS2, SELP, TNF, IL6, APP, ACHE, MAOA and ADA. Functional enrichment analyses of the candidate targets revealed their primary roles in regulating cytokine production, inflammatory response, cytokine activity, and tumor necrosis factor receptor binding. In vivo, ECS improved hippocampal neuroinflammation in the mouse model. Specifically, ECS reduced the expression of inflammatory factors in the hippocampus, inhibited M1 microglial cell polarization, and alleviated depression through the regulation of the NF-κB-NLRP3 inflammation pathway.Conclusion: Based on experimental and network analysis, this study revealed for the first time that ECS exerted antidepression effect via anti-neuroinflammation. Our research provides valuable information on the use of ECS as an alternative therapeutic approach for depression.

show abstract

“…It has been con rmed that EW improves neurobehaviors in rodents with middle artery occlusion-reperfusion injury and has speci c preventive and therapeutic effects in treating stroke [5]. EW can also markedly decrease the cytokine expressions for in ammation, such as TNF-α, IL-6 and IL-1β in the brain, decrease apoptosis, and improve nervous system symptoms related to in ammation [5][6][7]. The EW basic formulation consist of 10 drugs for the treatment of nervous system diseases, selected from EW formulation based on Mongolian medical theory.…”

Section: Introductionmentioning

confidence: 99%

Study on the Mechanism of Eerdun Wurile's Effects on Post-operative Cognitive Dysfuntion by the TLR4/NF-κB Pathway

Qiao¹,

et al. 2023

Preprint

View full text Add to dashboard Cite

Aims: The object of our work was to observe whether the Mongolian medicine Eerdun Wurile (EW) improve POCD by affecting the TLR4/NF-κB. Methods: Mice (6–8 week-old male C57BL/6J) were selected to establish an animal model of POCD by combining intracerebroventricular injection of lipopolysaccharide and nephrectomy; EW formulation and EW basic formulation were administered intra-gastrically for 7 consecutive days. The cognitive performance was assessed by Morris water maze test. H&E staining was examined to detect alterations in hippocampal tissue. Immunohistochemical staining was performed to evaluate MyD88, NF-κB, TLR4, iNOS, and IBA-1 expressions; Western blotting and RT-qPCR were performed to evaluate MyD88, NF-κB and TLR4. The expressions of IL-6, IL-1β, and TNF-α were evaluated by ELISA. Results: Intracerebroventricular injection of lipopolysaccharide combined with nephrectomy induced cognitive dysfunction in mice, stimulated TLR4/NF-κB and microglia, and promoted the secretion of murine TNF-α, IL-1β and IL-6. EW formulation and EW basic formulation treatment are able to suppress the TLR4/NF-κB pathway activation and microglia, and the serum cytokine secretions related to proinflammation, and restore the cognitive performance. Conclusion: EW formulation and EW basic formulation can improve POCD in mice, and TLR4/NF-κB pathway seems to be one of the important mechanisms in EW's improvement of POCD.

show abstract

Synergistic Regulation of Microglia Gene Expression by Natural Molecules in Herbal Medicine

Cited by 4 publications

References 39 publications

Study on the Mechanism of Eerdun Wurile’s Effects on Post-operative Cognitive Dysfunction by the TLR4/NF-κB Pathway

Study on the Mechanism of Eerdun Wurile’s Effects on Post-operative Cognitive Dysfunction by the TLR4/NF-κB Pathway

Investigation of anti-depression effects and potential mechanisms of the ethyl acetate extract of Cynomorium songaricum Rupr. through the integration of in vivo experiments, LC-MS/MS chemical analysis, and a systems biology approach

Study on the Mechanism of Eerdun Wurile's Effects on Post-operative Cognitive Dysfuntion by the TLR4/NF-κB Pathway

Contact Info

Product

Resources

About