Synergistic pH effect for reversible shuttling aptamer-based biosensors between graphene oxide and target molecules

Huang, Po‐Jung Jimmy; Kempaiah, Ravindra; Liu, Juewen

doi:10.1039/c1jm11702e

Cited by 56 publications

(38 citation statements)

References 34 publications

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“…Since under these conditions, the affinity between the aptamer and GO is too strong. 19,50 Finally, the time-dependent sensor signal was followed and the signal was stable over a week ( Figure S8). Response of the freshly prepared and regenerated covalent sensor with 0 or 2 mM ATP.…”

Section: Detection In Buffermentioning

confidence: 99%

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

et al. 2014

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Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. Signal is generated by analyte-induced probe desorption. To address non-specific probe displacement and false positive signal, we herein report a covalently linked aptamer probe for ATP detection. A fluorophore and amino dual-modified aptamer was linked to the carboxyl group on GO with a coupling efficiency of ~50%. The linearity, specificity, stability, and regeneration of the covalent sensor was systematically studied and compared to the physisorbed probe. Both sensors have similar sensitivity, but the covalent one is more resistant to non-specific probe displacement by proteins. The covalent sensor has a dynamic range from 0.125 mM to 2 mM ATP in buffer at room temperature and is resistance to DNase I. Intracellular ATP imaging was demonstrated using the covalent sensor, which generated higher fluorescence signal than the physisorbed sensor. After stimulating the cells with 5 mM Ca 2+ for ATP production, the intracellular signal enhanced by 31.8%. This work highlights the advantages of covalent aptamer sensors using GO as both a quencher and a delivery vehicle for intracellular metabolite detection.3

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Section: Detection In Buffermentioning

confidence: 99%

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

et al. 2014

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“…Hydrogen bonding can also be 11 disrupted at high pH due to deprotonation of hydrogen bond donors, which may explain the more desorption from GO. We did not try acidic pH since low pH would increase the adsorption affinity, 44 and thus no desorption is expected. Although electrostatic attraction is unlikely to take place between DNA and these materials, we still screened the charge interaction by adding 3 M NaCl.…”

Section: Dna Desorptionmentioning

confidence: 99%

Comparison of MoS₂, WS₂, and Graphene Oxide for DNA Adsorption and Sensing

et al. 2017

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Interfacing DNA with two-dimensional (2D) materials has been intensely researched for various analytical and biomedical applications. Most of such studies were performed on graphene oxide (GO), and two metal dichalcogenides, MoS2 and WS2; all of them can all adsorb single-stranded DNA. However, they like use different surface forces for adsorption based on their chemical structures. In this work, fluorescently labeled DNA oligonucleotides were used and their adsorption capacity and kinetics were studied as a function of ionic strength, DNA length and sequence. Desorption of DNA from these surfaces were also measured. DNA is more easily desorbed from GO by various denaturing agents, while surfactants yield more desorption from MoS2 and WS2. Our results are consistent with that DNA can be adsorbed by GO via π-π stacking and hydrogen bonding, MoS2 and WS2 mainly use van der Waals force for adsorption. Finally, fluorescent DNA probes were adsorbed by these 2D materials for detecting the complementary DNA. For this assay, GO gave the highest sensitivity, while they all showed a similar detection limit. This study has enhanced our fundamental understanding of DNA adsorption by two important types of 2D materials and is useful for further rational optimization of their analytical and biomedical applications.3

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“…At pH 3.5, desorbed aptamers can re-adsorb, releasing target analytes. The probe/GO complexes were harvested after centrifugation for regenerate [74].…”

Section: Adsorbed Dna or Aptamer Probesmentioning

confidence: 99%

Fluorescent sensors using DNA-functionalized graphene oxide

et al. 2014

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In the past few years, graphene oxide (GO) has emerged as a unique platform to develop DNA-based biosensors. This takes advantage of the DNA adsorption and fluorescence quenching property of GO.The adsorbed DNA probes can be desorbed from the GO surface in the presence of target analytes, producing fluorescence signal. In addition to this initial design, many other strategies have been reported including the use of aptamers, molecular beacons, and DNAzymes as probes, label-free detection, using the intrinsic fluorescence of GO and covalently linked DNA probes. The potential applications of DNA-functionalized GO range from environmental monitoring, cell imaging and biomedical diagnosis. In this review, we first summarize the fundamental surface interactions between DNA and GO and the related fluorescence quenching mechanism. Following that, the various sensor design strategies are critically compared. Problems at the current stages are described and a few future directions are also discussed.

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Synergistic pH effect for reversible shuttling aptamer-based biosensors between graphene oxide and target molecules

Cited by 56 publications

References 34 publications

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

Comparison of MoS₂, WS₂, and Graphene Oxide for DNA Adsorption and Sensing

Fluorescent sensors using DNA-functionalized graphene oxide

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Synergistic pH effect for reversible shuttling aptamer-based biosensors between graphene oxide and target molecules

Cited by 56 publications

References 34 publications

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

Comparison of MoS2, WS2, and Graphene Oxide for DNA Adsorption and Sensing

Fluorescent sensors using DNA-functionalized graphene oxide

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Comparison of MoS₂, WS₂, and Graphene Oxide for DNA Adsorption and Sensing