Synergistic interplay of ABA and BR signal in regulating plant growth and adaptation

Li, Qianqian; Xu, Fan; Chen, Zhuo; Teng, Zhenfeng; Sun, Kai; Li, Xiancai; Yu, Jianyuan; Liang, Yan; Huang, Xiahe; Du, Lin; Qian, Yangwen; Wang, Yingchun; Chu, Chengcai; Tang, Jiuyou

doi:10.1038/s41477-021-00959-1

Cited by 57 publications

(42 citation statements)

References 69 publications

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“…Plants have evolved complex regulatory hormonal signaling networks to respond and adapt to drought conditions. ABA has emerged as a crucial regulator of the drought response (C. Li et al ., 2021; J. Li et al ., 2021; Q. Li et al ., 2021). bHLH TFs are involved in regulating ABA signaling to help plants cope with drought stress (Hao et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

“…The basic helix–loop–helix (bHLH) superfamily, the second largest transcription factor (TF) family, is widely present in eukaryotes (Pires & Dolan, 2010). bHLH TFs are classified into six subgroups, A, B, C, D, E and F, based on their phylogenetic relationships and DNA binding functions; most plant bHLH proteins belong to subgroups A and B. Subgroup A members specifically bind to the E‐box core sequence in the promoters of their target genes, but subgroup B members preferentially bind to the G‐box sequence (Atchley & Fitch, 1997; C. Li et al ., 2021; J. Li et al ., 2021; Q. Li et al ., 2021). bHLH proteins usually consist of c .…”

Section: Introductionmentioning

confidence: 99%

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The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

et al. 2022

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Drought limits crop development and yields. bHLH (basic helix-loop-helix) transcription factors play critical roles in regulating the drought response in many plants, but their roles in this process in sweet potato are unknown.Here, we report that two bHLH proteins, IbbHLH118 and IbbHLH66, play opposite roles in the ABA-mediated drought response in sweet potato. ABA treatment repressed IbbHLH118 expression but induced IbbHLH66 expression in the drought-tolerant sweet potato line Xushu55-2. Overexpressing IbbHLH118 reduced drought tolerance, whereas overexpressing IbbHLH66 enhanced drought tolerance, in sweet potato.IbbHLH118 directly binds to the E-boxes in the promoters of ABA-insensitive 5 (IbABI5), ABA-responsive element binding factor 2 (IbABF2) and tonoplast intrinsic protein 1 (IbTIP1) to suppress their transcription. IbbHLH118 forms homodimers with itself or heterodimers with IbbHLH66. Both of the IbbHLHs interact with the ABA receptor IbPYL8. ABA accumulates under drought stress, promoting the formation of the IbPYL8-IbbHLH66-IbbHLH118 complex. This complex interferes with IbbHLH118's repression of ABA-responsive genes, thereby activating ABA responses and enhancing drought tolerance.These findings shed light on the role of the IbPYL8-IbbHLH66-IbbHLH118 complex in the ABA-dependent drought response of sweet potato and identify candidate genes for developing elite crop varieties with enhanced drought tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

et al. 2022

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“…The results of gene ontology (GO) enrichment analysis demonstrated that genes related to auxin signal transduction and BR biosynthesis were significantly overrepresented (Figure 5C), which implies that OsARF4 might be involved in auxin signal transduction and BR biosynthesis. In previous studies, genes related to regulation of leaf inclination, including OsBZR2 (Min et al, 2019), OsREM4.1 (Li et al, 2021), OsNCED5 (Zhu et al, 2009), OsBZR1 (Bai et al, Frontiers in Plant Science 10 frontiersin.org 2007), OsDWARF (Hong et al, 2002), OsD11 (Tanabe et al, 2005), OsD2 (Hong et al, 2003) OsGSR1 (Wang et al, 2009), OsBRI1 (Brassinazole-resistant 1; Yamamuro et al, 2000), and OsLPA1 (Wu et al, 2013), have been reported, and these genes showed variable expression in LJs of osarf4 mutant and OsARF4overexpressing line compared with that in WT/DJ (Figure 5D and Supplementary Figure 4). These results indicate the importance of OsARF4 in BR signaling.…”

Section: Rna-seq Analysis Of Wt/dj Mutant and Overexpression Line Of ...mentioning

confidence: 99%

OsARF4 regulates leaf inclination via auxin and brassinosteroid pathways in rice

et al. 2022

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Leaf inclination is a vital agronomic trait and is important for plant architecture that affects photosynthetic efficiency and grain yield. To understand the molecular mechanisms underlying regulation of leaf inclination, we constructed an auxin response factor (arf) rice mutant—osarf4—showing increased leaf inclination using CRISPR/Cas9 gene editing technology. OsARF4 encodes a nuclear protein that is expressed in the lamina joint (LJ) at different developmental stages in rice. Histological analysis indicated that an increase in cell differentiation on the adaxial side resulted in increased leaf inclination in the osarf4 mutants; however, OsARF4-overexpressing lines showed a decrease in leaf inclination, resulting in erect leaves. Additionally, a decrease in the content and distribution of indole-3-acetic acid (IAA) in osarf4 mutant led to a greater leaf inclination, whereas the OsARF4-overexpressing lines showed the opposite phenotype with increased IAA content. RNA-sequencing analysis revealed that the expression of genes related to brassinosteroid (BR) biosynthesis and response was different in the mutants and overexpressing lines, suggesting that OsARF4 participates in the BR signaling pathway. Moreover, BR sensitivity assay revealed that OsARF4-overexpressing lines were more sensitive to exogenous BR treatment than the mutants. In conclusion, OsARF4, a transcription factor in auxin signaling, participates in leaf inclination regulation and links auxin and BR signaling pathways. Our results provide a novel insight into l leaf inclination regulation, and have significant implications for improving rice architecture and grain yield.

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“…These primary signaling events are the initial elements of stress tolerance at the cell level and provide vital clues to acclimation mechanisms in stress-tolerant genotypes. For example, abscisic acid (ABA) plays a central role in response to drought [7], salinity [8] and extreme temperatures [9] and triggers many acclimation responses in plants [10]. However, downstream secondary molecular and metabolic events are vastly diverse and can be peculiar to one or more stress factors [11].…”

Section: Introductionmentioning

confidence: 99%

Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars

Habibpourmehraban

et al. 2022

IJMS

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Rice crops are often subject to multiple abiotic stresses simultaneously in both natural and cultivated environments, resulting in yield reductions beyond those expected from single stress. We report physiological changes after a 4 day exposure to combined drought, salt and extreme temperature treatments, following a 2 day salinity pre-treatment in two rice genotypes—Nipponbare (a paddy rice) and IAC1131 (an upland landrace). Stomata closed after two days of combined stresses, causing intercellular CO2 concentrations and assimilation rates to diminish rapidly. Abscisic acid (ABA) levels increased at least five-fold but did not differ significantly between the genotypes. Tandem Mass Tag isotopic labelling quantitative proteomics revealed 6215 reproducibly identified proteins in mature leaves across the two genotypes and three time points (0, 2 and 4 days of stress). Of these, 987 were differentially expressed due to stress (cf. control plants), including 41 proteins that changed significantly in abundance in all stressed plants. Heat shock proteins, late embryogenesis abundant proteins and photosynthesis-related proteins were consistently responsive to stress in both Nipponbare and IAC1131. Remarkably, even after 2 days of stress there were almost six times fewer proteins differentially expressed in IAC1131 than Nipponbare. This contrast in the translational response to multiple stresses is consistent with the known tolerance of IAC1131 to dryland conditions.

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Synergistic interplay of ABA and BR signal in regulating plant growth and adaptation

Cited by 57 publications

References 69 publications

The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

OsARF4 regulates leaf inclination via auxin and brassinosteroid pathways in rice

Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars

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